Role and mechanism of mitochondrial deoxyguanosine kinase in lung cancer tumorigenesis

J, Sun; Hu, Yuanzhao; Bai, Weiyu; Sun, Rui; Shang, RongLei

doi:10.1360/ssv-2019-0089

Cited by 3 publications

(6 citation statements)

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“…In our previous studies, we found that DGUOK deletion affected the assembly of mitochondrial complex I, and robustly inhibited lung adenocarcinoma tumor growth, metastasis, and CSC self-renewal(33, 34). We also found DGUOK regulates nicotinamide adenine dinucleotide (NAD + ) biosynthesis independent of the mitochondrial complex I (35).…”

Section: Resultsmentioning

confidence: 94%

The mitochondrial deoxyguanosine kinase is required for female fertility in mice

Gao,

Dong,

Yan

et al. 2023

Preprint

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Mitochondrial homeostasis plays a pivotal role in oocyte maturation and embryonic development. Deoxyguanosine kinase (DGUOK) is a nucleoside kinase that salvages purine nucleoside in the mitochondria and is critical for mitochondrial DNA replication and homeostasis in non-proliferating cells.Dguokloss-of-function mutations and deletions lead to hepatocerebral mitochondrial DNA deletion syndrome. However, its potential role in reproduction remains largely unknown. We found thatDguokknockout results in female infertility. Mechanistically, the deficiency of DGUOK hinders ovarian development and oocyte maturation. Moreover,Dguokdeficiency in oocytes caused a significant reduction in mitochondrial DNA and abnormal mitochondrial dynamics, and impaired germinal vesicle breakdown. Only few DGUOK-deficient oocytes were able to extrude the first polar body duringin vitromaturation, and these oocytes showed irregular chromosome arrangement and different spindle lengths. In addition,Dguokdeficiency elevated reactive oxygen species and accelerated apoptosis in oocytes. Our findings reveal novel physiological roles for the mitochondrial nucleoside salvage pathway in oocyte maturation and implicate that DGUOK is a potential marker for the diagnosis of female infertility.

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Section: Resultsmentioning

confidence: 94%

The mitochondrial deoxyguanosine kinase is required for female fertility in mice

Gao,

Dong,

Yan

et al. 2023

Preprint

Self Cite

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show abstract

“…In our previous studies, we found that DGUOK deletion affected the assembly of mitochondrial complex I and robustly inhibited lung adenocarcinoma tumor growth and metastasis and CSC self-renewal [ 34 , 35 ]. We also found that DGUOK regulated nicotinamide adenine dinucleotide (NAD + ) biosynthesis independent of mitochondrial complex I [36] .…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial deoxyguanosine kinase is required for female fertility in mice

Gao,

Dong,

Yan

et al. 2024

ABBS

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Mitochondrial homeostasis plays a pivotal role in oocyte maturation and embryonic development. Deoxyguanosine kinase (DGUOK) is a nucleoside kinase that salvages purine nucleosides in mitochondria and is critical for mitochondrial DNA replication and homeostasis in non-proliferating cells. Dguok loss-of-function mutations and deletions lead to hepatocerebral mitochondrial DNA deletion syndrome. However, its potential role in reproduction remains largely unknown. In this study, we find that Dguok knockout results in female infertility. Mechanistically, DGUOK deficiency hinders ovarian development and oocyte maturation. Moreover, DGUOK deficiency in oocytes causes a significant reduction in mitochondrial DNA copy number and abnormal mitochondrial dynamics and impairs germinal vesicle breakdown. Only few DGUOK-deficient oocytes can extrude their first polar body during in vitro maturation, and these oocytes exhibit irregular chromosome arrangements and different spindle lengths. In addition, DGUOK deficiency elevates reactive oxygen species levels and accelerates oocyte apoptosis. Our findings reveal novel physiological roles for the mitochondrial nucleoside salvage pathway in oocyte maturation and implicate DGUOK as a potential marker for the diagnosis of female infertility.

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“…Figura 4.7: A: Western blot mostró que dguok estaba completamente eliminado en H1650;D: Western blot mostró que dguok fue eliminado por completo en A549. Imagen tomada de [29].…”

Section: La Importancia Del Gen Dguokunclassified

“…Figura 4.8: B: La eliminación de DGUOK inhibió la formación de esferas de células H1650; C: Los datos cuantifican que el knockout de DGUOK inhibe la formación de esferas celulares H1650 ; E: La desactivación de DGUOK inhibió la formación de esferas de células A549 ; F: Los datos cuantificaron que la desactivación de DGUOK inhibía la formación de esferas de células A549. Imagen tomada de [29].…”

Section: La Importancia Del Gen Dguokunclassified

“…En otro estudio llevado a cabo por Rui Sun [29] se seleccionaron las células pulmonares H1650 y A549 (células de cáncer de pulmón de células no pequeñas ) como sujetos experimentales, y se eliminó el gen DGUOK vía la tecnología CRISPR/Cas9 . A través de la verificación de transferencia Western (Figura 4.7 A y D), se encontró que DGUOK no pudo detectar la señal en las células knockout 17 , lo que indica que el experimento construyó con éxito el knockout DGUOK.…”

Section: La Importancia Del Gen Dguokunclassified

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Propiedades espectrales de redes de regulación genética en cáncer

Juárez Toribio

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Role and mechanism of mitochondrial deoxyguanosine kinase in lung cancer tumorigenesis

Abstract: Articles you may be interested in BCI induces apoptosis via generation of reactive oxygen species and activation of intrinsic mitochondrial pathway in H1299 lung cancer cells

Cited by 3 publications

References 3 publications

The mitochondrial deoxyguanosine kinase is required for female fertility in mice

The mitochondrial deoxyguanosine kinase is required for female fertility in mice

Mitochondrial deoxyguanosine kinase is required for female fertility in mice

Propiedades espectrales de redes de regulación genética en cáncer

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