Role and Regulation of the Flp/Tad Pilus in the Virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193

Nykyri, Johanna; Mattinen, Laura; Niemi, Outi; Adhikari, Satish; Kõiv, Viia; Somervuo, Panu; Fang, Xin; Auvinen, Petri; Mäe, Andres; Palva, E. Tapio; Pirhonen, Minna

doi:10.1371/journal.pone.0073718

Cited by 41 publications

(30 citation statements)

References 42 publications

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“…While the presence of type I fimbriae is a specific feature of D. aquatica among Dickeya , the Flp/Tad pilus, involved in plant surface adherence (Nykryri et al ., ), is restricted to D. chrysanthemi likely as the consequence of a HGT from another proteobacterium (Supporting Information Table S4 and Fig. S3).…”

Section: Resultsmentioning

confidence: 99%

The phytopathogenic nature of Dickeya aquatica 174/2 and the dynamic early evolution of Dickeya pathogenicity

Duprey

Taïb

Léonard

et al. 2019

Environmental Microbiology

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Summary Dickeya is a genus of phytopathogenic enterobacterales causing soft rot in a variety of plants (e.g. potato, chicory, maize). Among the species affiliated to this genus, Dickeya aquatica, described in 2014, remained particularly mysterious because it had no known host. Furthermore, while D. aquatica was proposed to represent a deep‐branching species among Dickeya genus, its precise phylogenetic position remained elusive. Here, we report the complete genome sequence of the D. aquatica type strain 174/2. We demonstrate the affinity of D. aquatica strain 174/2 for acidic fruits such as tomato and cucumber and show that exposure of this bacterium to acidic pH induces twitching motility. An in‐depth phylogenomic analysis of all available Dickeya proteomes pinpoints D. aquatica as the second deepest branching lineage within this genus and reclassifies two lineages that likely correspond to new genomospecies (gs.): Dickeya gs. poaceaephila (Dickeya sp NCPPB 569) and Dickeya gs. undicola (Dickeya sp 2B12), together with a new putative genus, tentatively named Prodigiosinella. Finally, from comparative analyses of Dickeya proteomes, we infer the complex evolutionary history of this genus, paving the way to study the adaptive patterns and processes of Dickeya to different environmental niches and hosts. In particular, we hypothesize that the lack of xylanases and xylose degradation pathways in D. aquatica could reflect adaptation to aquatic charophyte hosts which, in contrast to land plants, do not contain xyloglucans.

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Section: Resultsmentioning

confidence: 99%

The phytopathogenic nature of Dickeya aquatica 174/2 and the dynamic early evolution of Dickeya pathogenicity

Duprey

Taïb

Léonard

et al. 2019

Environmental Microbiology

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“…Dickeya solani Tn 5 mutants showing at least 1.5‐fold (equivalent to 150% increase) change in the relative gene expression at 18 °C compared to gene expression at 37 °C (and vice versa; Table S1) were screened for various phenotypic features: swimming and swarming motility (Czajkowski et al ., ), the ability to grow on the TSA medium supplemented with 5% NaCl (Dickey, ), production of auxins (Ehmann, ), cellulases (Py et al ., ), phospholipase (Rigby, ), proteases (Ji et al ., ), pectinolytic enzymes (Pérombelon & van der Wolf, ) and siderophores (Schwyn & Neilands, ), hypersensitive response on tobacco leaves (Fahy & Persley, ), ability to cause rotting of chicory leaves (Krzyzanowska et al ., ) and potato tubers (Czajkowski et al ., ) and for ability to form biofilm (Nykyri et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Temperature‐responsive genetic loci in pectinolytic plant pathogenic Dickeya solani

et al. 2016

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Fifty-four Dickeya solani thermoregulated genes were identified using Tn5 transposon mutagenesis with an inducible gusA reporter system; 45 genes were up-regulated at 37°C, whereas nine were up-regulated at 18°C. The relative level of gene up-regulation ranged from 2-1200 and 5-650 U/mg total proteins at 18 and 37°C, respectively. Among the temperature-regulated loci, genes coding for proteins involved in fundamental bacterial metabolism, membrane-related proteins and pathogenicity-corresponding factors and several hypothetical unknown proteins were found. The mutants were tested for their pathogenicity in planta and for features known to be important for D. solani virulence viz. production of pectinolytic enzymes, cellulases, proteases, siderophores and auxins as well as for motility and the ability to form a biofilm. Eight Tn5 mutants, four up-regulated at high and four up-regulated at low temperature, expressed visible phenotypes including the decreased ability to cause symptoms on potato tubers and chicory leaves, impairment in phospholipase production and/or deficiency in biofilm formation. The implications of environmental temperature on the ability of D. solani to cause disease symptoms in potato are discussed.

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“…In the three genomes, we also identified three tight adherence ( tad ) systems. These systems have been reported as adherence, attachment, and colonization factors in a wide variety of bacteria (Motherway et al., ; Nykyri et al., ; Pu & Rowe‐magnus, ). Their role was initially assessed in silico and experimentally confirmed in some PGPB species, such as Pseudomonas chlororaphis and Mesorhizobium amorphae (Chen et al., ; Haq, Graupner, Nazir, & van Elsas, ; Shen, Hu, Peng, Wang, & Zhang, ).…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics of Paraburkholderia kururiensis and its potential in bioremediation, biofertilization, and biocontrol of plant pathogens

et al. 2019

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Burkholderia harbors versatile Gram‐negative species and is β‐Proteobacteria. Recently, it was proposed to split the genus in two main branches: one of animal and plant pathogens and another, Paraburkholderia, harboring environmental and plant‐beneficial species. Currently, Paraburkholderia comprises more than 70 species with ability to occupy very diverse environmental niches. Herein, we sequenced and analyzed the genome of Paraburkholderia kururiensis type strain KP23T, and compared to P. kururiensis M130, isolated in Brazil, and P. kururiensis susbp. thiooxydans, from Korea. This study focused on the gene content of the three genomes with special emphasis on their potential of plant‐association, biocontrol, and bioremediation. The comparative analyses revealed several genes related to plant benefits, including biosynthesis of IAA, ACC deaminase, multiple efflux pumps, dioxygenases, and degradation of aromatic compounds. Importantly, a range of genes for protein secretion systems (type III, IV, V, and VI) were characterized, potentially involved in P. kururiensis well documented ability to establish endophytic association with plants. These findings shed light onto bacteria‐plant interaction mechanisms at molecular level, adding novel information that supports their potential application in bioremediation, biofertilization, and biocontrol of plant pathogens. P. kururiensis emerges as a promising model to investigate adaptation mechanisms in different ecological niches.

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Role and Regulation of the Flp/Tad Pilus in the Virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193

Cited by 41 publications

References 42 publications

The phytopathogenic nature of Dickeya aquatica 174/2 and the dynamic early evolution of Dickeya pathogenicity

The phytopathogenic nature of Dickeya aquatica 174/2 and the dynamic early evolution of Dickeya pathogenicity

Temperature‐responsive genetic loci in pectinolytic plant pathogenic Dickeya solani

Comparative genomics of Paraburkholderia kururiensis and its potential in bioremediation, biofertilization, and biocontrol of plant pathogens

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