Role and underlying mechanisms of the interstitial protein periostin in the diagnosis and treatment of malignant tumors (Review)

Ye, Dong; Shen, Zhi Sen; Qiu, Shi; Li, Qun; Wang, Guo Li

doi:10.3892/ol.2017.6866

Cited by 5 publications

(5 citation statements)

References 59 publications

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“…Invasion and metastasis are the fundamental biological features of malignancies, leading to poor prognosis or even death in patients (Ye et al, 2017). In this study, miR‐93‐5p mimics and si‐PD‐L1 were discovered to weaken the migration and invasion of SW480 cells, but miR‐93‐5p inhibitor‐induced totally different effects.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

Chen

Wang

Lin

et al. 2020

Cell Biology International

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This work aimed to investigate miR-93-5p expression in tumor tissue and its in vitro effects in colorectal cancer (CRC) by targeting programmed death ligand-1 (PD-L1). MiR-93-5p and PD-L1 expression was detected in CRC and adjacent normal tissues by quantitative real-time polymerase chain reaction and immunohistochemistry. The correlation between miR-93-5p and PD-L1 was validated by a dual-luciferase reporter assay. HCT116 and SW480 cells were divided into blank, miR-NC, miR-93-5p mimics, miR-93-5p inhibitor, PD-L1 small interfering RNA (siRNA) and miR-93-5p inhibitor + PD-L1 siRNA groups, and wound-healing and transwell assays were performed to detect cell migration and invasion, respectively. Protein expression was measured by western blotting. The secretion of cytokines was detected in the CRC cell/T coculture models. MiR-93-5p was downregulated in CRC tissues with upregulated PD-L1. In PD-L1-negative patients, miR-93-5p expression was increased compared with that in PD-L1-positive patients. MiR-93-5p and PD-L1 expression levels were associated with the tumor differentiation, lymphatic metastasis, TNM, Duke's stage, and prognosis of CRC. PD-L1 siRNA weakened the migration and invasion abilities via decreased expression of matrix metalloproteinase-1 (MMP-1), -2, and -9, and these effects were abolished by the miR-93-5p inhibitor. Additionally, anti-PD-L1 upregulated the expressions of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α), and interferon γ (IFN-γ) in the coculture of T cells with CRC cells, but downregulated the expressions of IL-1β, IL-10, and TGF-β. However, these changes were partially reversed by miR-93-5p inhibition. miR-93-5p is expected to be a novel target for CRC treatment since it decreases the migration and invasion, as well as the immune evasion, of CRC cells via targeting PD-L1. Abbreviations: CRC, colorectal cancer; ELISA, enzyme-linked immunosorbent assay; HE, hematoxylin and eosin; ITIM, immunoreceptor tyrosinebased inhibitory motif; miRNA, microRNA; PD-L1, programmed death ligand-1 Role of miR-93-5p in CRC via targeting PD-L1Y.-L. Chen et al.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

Chen

Wang

Lin

et al. 2020

Cell Biology International

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“…Therefore, it is logical to postulate that p53 and pRb play a vital role in RBBP6‐mediated PCa. Invasion and metastasis are the fundamental biological features of malignancies, leading to poor prognosis or even death in patients (Ye, Shen, Qiu, Li, & Wang, 2017). Yoshitake et al (2004) reported a significant upregulation of RBBP6 enhanced tumor cell proliferation in invasive esophageal cancer.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the expression and association of retinoblastoma binding protein 6 with the JNK signaling pathway in prostate cancers

Guan

Zhao

Luo

2020

Cell Biology International

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This study aims to investigate the expression of retinoblastoma binding protein 6 (RBBP6) in prostate cancer (PCa) and its association with the c‐Jun N‐terminal kinase (JNK) pathway. Immunohistochemistry was used to detect RBBP6 and JNK1/2 expression in PCa and benign prostatic hyperplasia tissues. RBBP6 expression in PCa cells (LNCap, PC3, and DU145) and noncancerous prostate epithelial cells (RWPE‐1) was determined by quantitative real‐time polymerase chain reaction and western blot analysis. PC3 and DU145 cells were transfected with RBBP6 small interfering RNAs (siRNAs) to examine the biological characteristics. Anisomycin (a JNK activator) with/without RBBP6 siRNA was used to treat PC3 cells for further investigating the ramification of the RBBP6‐mediated JNK pathway in PCa. PCa tissues and cells showed higher RBBP6 and JNK1/2 expression. RBBP6 was positively correlated with JNK1/2 in PCa tissues. Besides, RBBP6 expression was correlated to clinical tumor stage, lymph node metastasis, Gleason grade, preoperative prostate‐specific antigen level, as well as prognosis of PCa. RBBP6 siRNA reduced cell proliferation, arrested cells at G2/M, and promoted cell apoptosis, and suppressed JNK pathway. In addition, migration and invasion decreased after the RBBP6 siRNA transfection with downregulated matrix metallopeptidase‐2 (MMP‐2) and MMP‐9. Anisomycin promoted the proliferation, invasion, and migration of PC3 cells and inhibited PC3 cell apoptosis, which could be reversed by RBBP6 siRNA. RBBP6 expression was upregulated in PCa tissues and positively correlated with expression level of JNK1/2. With inhibition of RBBP6 expression, the proliferation, invasion, and migration of PCa cells decreased dramatically, while PC3 cell apoptosis increased appreciably, accompanied by the suppression of the JNK pathway.

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“…We consistently noted increased expression of periostin in non-lesional bone at the edge of growing benign and malignant bone tumours. It has been shown that periostin plays a role in the progression of inflammatory and neoplastic lesions [ 6 , 11 , 12 , 23 ]. Periostin is known to be expressed by fibroblasts in RA, carcinomas and other malignant tumours [ 26 – 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…It has been identified in rheumatoid arthritis (RA) and osteoarthritis (OA) joints [ 8 , 9 ] with a recent study identifying periostin as a key regulator in RA synoviocyte migration/invasion associated with pannus formation [ 10 ]. Periostin is also expressed in a number of cancers where it is thought, by various mechanisms, to play a role in tumour progression [ 3 , 6 , 11 , 12 ]. Periostin has been identified in a few bone tumours, including fibrous dysplasia and osteosarcoma [ 13 – 15 ], but its expression in other bone neoplasms has not been fully investigated.…”

Section: Introductionmentioning

confidence: 99%

Periostin expression in neoplastic and non-neoplastic diseases of bone and joint

et al. 2018

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BackgroundPeriostin is a matricellular protein that is expressed in bone and joint tissues. To determine the expression of periostin in primary bone tumours and to assess whether it plays a role in tumour progression, we carried out immunohistochemistry and ELISA for periostin in a range of neoplastic and non-neoplastic bone and joint lesions.Methods140 formalin-fixed paraffin-embedded sections of bone tumours and tumour-like lesions were stained by an indirect immunoperoxidase technique with a polyclonal anti-periostin antibody. Periostin expression was also assessed in rheumatoid arthritis (RA) and non-inflammatory osteoarthritis (OA) synovium and synovial fluid immunohistochemistry and ELISA respectively.ResultsPeriostin was most strongly expressed in osteoid/woven bone of neoplastic and non-neoplastic bone-forming lesions, including osteoblastoma, osteosarcoma, fibrous dysplasia, osteofibrous dysplasia, fracture callus and myositis ossificans, and mineralised chondroid matrix/woven bone in chondroblastoma and clear cell chondrosarcoma. Reactive host bone at the edge of growing tumours, particularly in areas of increased vascularity and fibrosis, also stained strongly for periostin. Vascular elements in RA synovium strongly expressed periostin, and synovial fluid levels of periostin were higher in RA than OA.ConclusionsIn keeping with its known role in modulating the synthesis of collagen and other extracellular matrix proteins in bone, strong periostin expression was noted in benign and malignant lesions forming an osteoid or osteoid-like matrix. Periostin was also noted in other bone tumours and was found in areas of reactive bone and increased vascularity at the edge of growing tumours, consistent with its involvement in tissue remodelling and angiogenesis associated with tumour progression.

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Role and underlying mechanisms of the interstitial protein periostin in the diagnosis and treatment of malignant tumors (Review)

Cited by 5 publications

References 59 publications

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

MicroRNA‐93‐5p expression in tumor tissue and its tumor suppressor function via targeting programmed death ligand‐1 in colorectal cancer

Analysis of the expression and association of retinoblastoma binding protein 6 with the JNK signaling pathway in prostate cancers

Periostin expression in neoplastic and non-neoplastic diseases of bone and joint

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