Role forrpoSgene ofPseudomonas aeruginosain antibiotic tolerance

Abstract

The alternative sigma factor, RpoS has been described as a central regulator of many stationary phase-inducible genes and a master stress-response regulator under various stress conditions. We constructed an rpoS mutant in Pseudomonas aeruginosa and investigated the role of rpoS gene in antibiotic tolerance. The survival of the rpoS mutant cells in stationary phase was approximately 70 times lower when compared with that of the parental strain at 37 degrees C for 2 h after the addition of biapenem. For imipenem, the survival was approximately 40 times lower. Heat stress promoted an increase in the survival of the parental strain to biapenem, but the same was not found to be the case for the rpoS mutant. Our results indicate that rpoS gene is involved in tolerance to antibiotics in P. aeruginosa during the stationary phase and heat stress. However, under osmotic stress, tolerance to biapenem was not dependent on the rpoS gene.

MazF ribonucleases promote Mycobacterium tuberculosis drug tolerance and virulence in guinea pigs

“…3d). Factors such as quorum sensing, cell density, nutrient starvation, diauxic carbon source transitions, environmental signals (such as heat shock, oxidative stress and DNA damage) and stringent response pathways contribute to microbial persistence in vitro [54][55][56][57][58][59] . TA modules that are upregulated in persisters are terminal effectors of microbial persistence 60,61 .…”

Starvation- and antibiotics-induced formation of persister cells in Pseudomonas aeruginosa

“…In this context, it is interesting to note that carbapenems are strong inducers of ampC and genes encoding the RND efflux pumps (MexXY-OprM, as well as MexAB-OprM or MexCD-OprJ), and those with other mechanisms (e.g., loss of porin protein D) may contribute to carbapenem resistance 25 . Moreover, the rpoS mutant strain has been associated with increased susceptibility to carbapenems in stationary phase cells of P. aeruginosa 6 . This implies the involvement of RpoS in carbapenem resistance.…”

“…4 ) (repressor of the Pho operon and putative enzyme in phosphate metabolism), alternative sigma factor RpoN, dinG (putative DNA helicase), spuC (putrescin aminotransferase involved in polyamines metabolism), algR (global regulator of cell processes like alginate production; alginate biosynthesis regulatory protein), pilH (type IV pilus response regulator), ycgM (putative fumarylacylacetoacetate hydrolase), pheA (fused chorismate mutase-prephenate dehydratase involved in phenylalanine biosynthesis and metabolism), PA14_17880 (putative acetyl-CoA acetyltransferase, PA14_04150), dnpA (de-N-acetylase) and PA14_13680 (putative short-chain dehydrogenase) (ref. [5][6][7][8] ). Further cues like biofilm environment, toxins (RelE and MazF) (ref.…”

Essential Biological Processes of an Emerging Pathogen: DNA Replication, Transcription, and Cell Division in Acinetobacter spp

“…Unlike in most other organisms, however, this regulation was independent of the RecA protein, suggesting a novel mechanism for the DNA damage response in this organism. The second gene found to be upregulated following DNA damage in A. baylyi ADP1 is ddrR, which has no identifiable (143,184). Activation of these responses leads to upregulation of mutation-inducing error-prone DNA polymerases and increased rates of lateral gene transfer (80).…”