Role of a copper-specific metallothionein of the blue crab, Callinectes sapidus, in copper metabolism associated with degradation and synthesis of hemocyanin

Brouwer, Marius; Syring, Rachel; Brouwer, Thea Hoexum

doi:10.1016/s0162-0134(01)00381-6

Cited by 70 publications

(57 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, its release approach is still poorly studied. Real-time PCR results showed that the MNHc mRNA expression levels in hepatopancreas were closely related to the stage of molting and increased from post-molt and reached the highest in inter-molt stage C, which were similar to that of the hemocyanin gene of L. vannamei and Callinectes sapidus [17,18]. However, in many crustacean, the MNHc mRNA expression levels in hepatopancreas were significantly increased from post-molt and reached the highest level until pre-molt [18][19][20].…”

Section: Discussionmentioning

confidence: 87%

“…Molting of crustaceans is a complicated physiological process, apart from hormone (mainly ecdysone) regualtion, oxygen-consuming amount and osmotic pressure would significantly increase. Hemocyanin as the sole respiratory protein was also involved in physiological processes such as osmoregulation, protein storage and exoskeleton formation [18,19]. In addition, Eurypelma californicum hemocyanin could bind with ecdysone, participating in hormone transfer and molting regulation [21].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cloning and characterization of the hemocyanin gene of prawn Macrobrachium nipponense

Wang¹,

Xia²,

Liu³

et al. 2012

Turk J Bioch

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of the hemocyanin gene of prawn Macrobrachium nipponense

Wang¹,

Xia²,

Liu³

et al. 2012

Turk J Bioch

View full text Add to dashboard Cite

“…Ten genes (Table 1: genes 1 to 10), including heat shock protein 70 (Hsp70), mitochondrial and cytosolic manganese superoxide dismutase (mit-MnSOD and cyt-MnSOD) (Brouwer et al 2003), hemocyanin 1 (Brouwer et al 2002), Cu and Cd metallothionein (CuMT3 and CdMT1) (Syring et al 2000), beta-actin, and ribosomal proteins S15, L23 and S20, were cloned and sequenced from hepatopancreas tissue of blue crab. The blue crab genes were PCRamplified and then robotically spotted in duplicate onto neutral nylon membranes together with various controls, including exogenous Arabidopsis 'spiking' genes, as described elsewhere (Larkin et al 2003).…”

Section: Methodsmentioning

confidence: 99%

Molecular indicators of hypoxia in the blue crab Callinectes sapidus

Brown‐Peterson¹,

Larkin²,

Denslow³

et al. 2005

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

Occurrence of hypoxia in estuarine waters is increasing, and recovery of impacted estuaries is slow. Detection of early effects of hypoxia is needed for timely remedial action to be taken. Here we examine the use of hypoxia-responsive gene and protein expression profiles in the blue crab Callinectes sapidus as early indicators of impacts of hypoxia. We cloned 23 potential hypoxiaresponsive genes, which were used to construct gene macroarrays. Crabs exposed to chronic hypoxia (2.5 ppm dissolved oxygen [DO]) for 15 d showed significant (p < 0.05) changes in gene expression of heat shock protein 70 (Hsp70), copper metallothionein (CuMt3), cytosolic MnSOD (cyt-MnSOD) and ribosomal proteins S15 and L23. In all cases except for CuMt3, gene expression decreased after 5 d exposure to hypoxia. Expression of Hsp70, CuMt3 and cyt-MnSOD also increased (p < 0.05) in normoxic crabs held for 15 d, suggesting confounding effects from confinement stress. Hemocyanin protein concentrations changed significantly (p = 0.005) across the 15 d chronic hypoxia exposure. Hemocyanin in crabs exposed to 10 d intermittent hypoxia (2.5 to 8 ppm DO over a 24 h cycle) did not change, but cyt-MnSOD gene expression increased significantly (p = 0.037), whereas cytochrome c oxidase subunit 1 (ccox1) showed a 2.2-fold downregulation. Blue crabs collected from Pensacola Bay, Florida, showed significant (p < 0.006) downregulation of ccox1 and cyt-MnSOD gene expression as well as hemocyanin protein levels at a diurnally hypoxic marsh site. Several hypoxiaresponsive genes (elongation factor 2, cryptocyanin, hemocyanin) were also significantly elevated (p < 0.006) in intermolt versus premolt normoxic crabs. The identification of hypoxia-responsive genes and proteins in the blue crab is a promising first step towards the development of sensitive molecular tools for the detection of sublethal effects of hypoxia in estuarine-resident species.KEY WORDS: Dissolved oxygen · Hypoxia · Crustacea · Molecular biomarker · Gene arrays Resale or republication not permitted without written consent of the publisher

show abstract

“…It has been postulated that copper metabolism in decapod crustaceans associated with degradation of Hc (during starvation or before molting) includes the processes of copper translocation to the hepatopancreas and storage in the form of complexes with glutathione and copper-binding proteins (Djangmah, 1970;Brouwer et al, 2002). Brouwer et al (2002) showed that metabolism of the copper-binding protein metallothionein in the hepatopancreas of blue crab was related to molt stage.…”

Section: Copper Content In the Hepatopancreasmentioning

confidence: 99%

“…Brouwer et al (2002) showed that metabolism of the copper-binding protein metallothionein in the hepatopancreas of blue crab was related to molt stage. Metallothionein isoform 3 was present in premolt and softshell crab and was absent in hepatopancreas of intermolt crab.…”

Section: Copper Content In the Hepatopancreasmentioning

confidence: 99%

Effects of pot fishing on the physical condition of snow crab (Chionoecetes opilio) and southern Tanner crab (Chionoecetes bairdi)

Моисеев¹,

Moiseeva²,

Ryazanova³

et al. 2013

View full text Add to dashboard Cite

Abstract-The effects of commercial fi shing with crab pots on the physical condition of the snow crab (Chionoecetes opilio) and southern Tanner crab (C. bairdi) were investigated in the Bering Sea and in Russian waters of the Sea of Okhotsk. In crabs that were subjected to pot hauling, the presence of gas embolism and the deformation of gill lamellae were found in histopathological investigations. Crab vitality, which was characterized subjectively through observation of behavioral responses, depended on not only the number of pot hauls but also the time between hauls. Immediately after repeated pot hauls at short time intervals (≤3 days), we observed a rapid decline in vitality of crabs. When hauling intervals were increased to >3 days, the condition of crabs did not significantly change. After repeated pot hauls, concentration of the respiratory pigment hemocyanin ([Hc]) was often lower in the hemolymph of crabs than in the hemolymph of freshly caught animals. Our research indicated that changes in [Hc] in crabs after repeated pot hauls were caused by the effects of decompression and not by starvation of crabs in pots or exposure of crabs to air. We suggest that the decrease in [Hc]

show abstract

Role of a copper-specific metallothionein of the blue crab, Callinectes sapidus, in copper metabolism associated with degradation and synthesis of hemocyanin

Cited by 70 publications

References 70 publications

Cloning and characterization of the hemocyanin gene of prawn Macrobrachium nipponense

Cloning and characterization of the hemocyanin gene of prawn Macrobrachium nipponense

Molecular indicators of hypoxia in the blue crab Callinectes sapidus

Effects of pot fishing on the physical condition of snow crab (Chionoecetes opilio) and southern Tanner crab (Chionoecetes bairdi)

Contact Info

Product

Resources

About