Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici

Thomashow, Linda S.; Weller, David M.

doi:10.1128/jb.170.8.3499-3508.1988

Cited by 644 publications

(416 citation statements)

References 54 publications

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“…Furthermore, they also show the coexistence of DAPG-and phenazine-producing Pseudomonas in a naturally suppressive soil. Although phenazineproducing strain P. fluorescens 2-79 was originally isolated from a take-all suppressive soil and was shown to efficiently suppress take-all disease through the production of phenazine-1-carboxylic acid (Thomashow and Weller, 1988), the role of phenazine-producing Pseudomonas populations in the natural soil suppressiveness to take-all and other soil-borne diseases could not be confirmed (Raaijmakers et al, 1997;Mavrodi et al, 2006). The higher density of phenazine-producing Pseudomonas in the suppressive soil of Châteaurenard compared with the conducive soil of Carquefou may be related to differences in the physicochemical properties of these two soils.…”

Section: Discussionmentioning

confidence: 99%

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

et al. 2009

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Natural disease-suppressive soils provide an untapped resource for the discovery of novel beneficial microorganisms and traits. For most suppressive soils, however, the consortia of microorganisms and mechanisms involved in pathogen control are unknown. To date, soil suppressiveness to Fusarium wilt disease has been ascribed to carbon and iron competition between pathogenic Fusarium oxysporum and resident non-pathogenic F. oxysporum and fluorescent pseudomonads. In this study, the role of bacterial antibiosis in Fusarium wilt suppressiveness was assessed by comparing the densities, diversity and activity of fluorescent Pseudomonas species producing 2,4-diacetylphloroglucinol (DAPG) (phlD+) or phenazine (phzC+) antibiotics. The frequencies of phlD+ populations were similar in the suppressive and conducive soils but their genotypic diversity differed significantly. However, phlD genotypes from the two soils were equally effective in suppressing Fusarium wilt, either alone or in combination with non-pathogenic F. oxysporum strain Fo47. A mutant deficient in DAPG production provided a similar level of control as its parental strain, suggesting that this antibiotic does not play a major role. In contrast, phzC+ pseudomonads were only detected in the suppressive soil. Representative phzC+ isolates of five distinct genotypes did not suppress Fusarium wilt on their own, but acted synergistically in combination with strain Fo47. This increased level of disease suppression was ascribed to phenazine production as the phenazine-deficient mutant was not effective. These results suggest, for the first time, that redox-active phenazines produced by fluorescent pseudomonads contribute to the natural soil suppressiveness to Fusarium wilt disease and may act in synergy with carbon competition by resident non-pathogenic F. oxysporum.

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Section: Discussionmentioning

confidence: 99%

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

et al. 2009

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“…While other strains have been accounted on controlling Gaeumannomyces graminis var. tritici on wheat (Thomashow and Weller 1988), Ralstonia solanacearum on tomato (Barret et al, 2009), stem rot disease caused by Phytium spp on cotton (Erdoğan, Bölek, & Göre, 2016). The combination of Pf and B. subtilis was able to reduce Plasmodiophora brassicae on chinese cabbage (Zhao et al, 2016) and Phytopthora capsici in pepper (Özyilmaz & Benlioglu, 2013).…”

Section: Plant Growth Improvement and White Rust Incidencementioning

confidence: 99%

Application of PGPR and Antagonist Fungi-based Biofungicide for White Rust Disease Control and Its Economyc Analysis in Chrysanthemum Production

Hanudin¹,

Budiarto²,

Marwoto³

2017

Agrivita.J.Agr.Sci

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Plant growth promoting rhizobacteria (PGPR) application in combination with other antagonist microbes as biopesticide have been considered in many crops. Our research was conducted to evaluate the efficacy of these useful combinations with the carrying agent for growth promotion, thus lowering white rust incidence in chrysanthemum production. The experiment was carried out at three cooperative farmer sites located in Cipanas, Cianjur, West Java, Indonesia from January to December 2016. The production process was arranged in a paired treatment; a combination of PGPR and antagonist fungi (without supplemental chemical fertilizers and fungicide), furtherly called biofungicide and common farmer practices. The results showed that the application of biofungicide promoted equal plant growth quality as common practices. White rust incidency was lower at biofungicide treatment sites, thus increased the markertable flowers quantity. The production cost was considered more efficient in biofungicide sites, due to cheaper price of biofungicide than chemical fertilizers and fungicide. The increase of marketable stalks and cost efficiency led to an increase of net income of biofungicide based production as also viewed from higher Revenue Cost Ratio (R/C) than common farmer practices.

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“…Inhibition of P. ultimum and G. graminis was assayed on KBA, basically as described by Thomashow & Weller (1988). Samples (5 mL; approximately 5 × 10 6 colony-forming units (CFU)) from overnight nutrient-yeast extract bacterial cultures were spotted onto agar plates at 1 cm from the edge (four spots per plate).…”

Section: Fungal Inhibition Assays In Vitromentioning

confidence: 99%

**The global regulator GacA of Pseudomonas fluorescens CHA0 is required for suppression of root diseases in dicotyledons but not in Gramineae**

1997

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Pseudomonas fluorescens strain CHA0 suppresses various plant diseases caused by soil‐borne fungi. The pseudomonad produces the antimicrobial metabolites 2,4‐diacetylphloroglucinol (Phl), pyoluteorin (Plt) and hydrogen cyanide, which are important for disease suppression, as well as the siderophores pyoverdine (Pvd), salicylic acid (Sal) and pyochelin (Pch). In the current work, a derivative of CHA0 with a mutation in the global regulator gene gacA (GacA−), which is unable to produce Phl, Plt and HCN, failed to protect the dicotyledonous plants cress and cucumber against damping‐off caused by Pythium ultimum. In contrast, the GacA− mutant could still protect the Gramineae wheat and maize against damping‐off mediated by the same strain of P. ultimum, and wheat against take‐all caused by Gaeumannomyces graminis. However, the GacA− mutant overproduced Pch and Pvd. To gain more insight into disease protection afforded by the GacA− mutant, a GacA− Pvd− double mutant (strain CHA496) was constructed by gene replacement. Strain CHA496 overproduced Pch and Sal compared with CHA0 and protected wheat against P. ultimum and G. graminis, whereas cress and cucumber were not protected. Addition of FeCl3 repressed Pch and Sal production by strain CHA496 in vitro and impaired the protection of wheat in soil microcosms. In conclusion, a functional gacA gene was necessary for the protection of dicotyledons against root diseases, but not for that of Gramineae. Results indicated also that Pch and/or Sal were involved in the ability of the GacA− Pvd− mutant of CHA0 to suppress root diseases in Gramineae.

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Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici

Cited by 644 publications

References 54 publications

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

Application of PGPR and Antagonist Fungi-based Biofungicide for White Rust Disease Control and Its Economyc Analysis in Chrysanthemum Production

**The global regulator GacA of Pseudomonas fluorescens CHA0 is required for suppression of root diseases in dicotyledons but not in Gramineae**

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