2009
DOI: 10.1111/j.1574-6968.2009.01604.x
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Role of a serine-type d-alanyl-d-alanine carboxypeptidase on the survival ofOchrobactrumsp. 11a under ionic and hyperosmotic stress

Abstract: The plant growth-promoting rhizobacterium, Ochrobactrum sp. 11a displays a high intrinsic salinity tolerance and has been used in this work to study the molecular basis of bacterial responses to high concentrations of NaCl. A collection of Ochrobactrum sp. 11a mutants was generated by Tn5-B21 mutagenesis and screened for sensitivity to salinity. One clone, designated PBP and unable to grow on glutamate mannitol salt agar medium supplemented with 300 mM NaCl was selected and further characterized. The PBP mutan… Show more

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Cited by 13 publications
(10 citation statements)
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“…Both of these proteins have been shown to have DD carboxypeptidase activity (29), which functions to cleave peptidoglycan cross-links. In Lactobacillus casei, salt stress leads to decreased peptidoglycan crosslinks (38), and DD carboxypeptidase is important for the adaptation of Ochrobactrum bacteria to high salinity (39). The DD carboxypeptidase lmo2754 protein level was significantly higher in L. monocytogenes at 4°C than when grown at 37°C (9), indicating that this may be the allele important for modifications at low temperature.…”
Section: Discussionmentioning
confidence: 93%
“…Both of these proteins have been shown to have DD carboxypeptidase activity (29), which functions to cleave peptidoglycan cross-links. In Lactobacillus casei, salt stress leads to decreased peptidoglycan crosslinks (38), and DD carboxypeptidase is important for the adaptation of Ochrobactrum bacteria to high salinity (39). The DD carboxypeptidase lmo2754 protein level was significantly higher in L. monocytogenes at 4°C than when grown at 37°C (9), indicating that this may be the allele important for modifications at low temperature.…”
Section: Discussionmentioning
confidence: 93%
“…Cell envelope-related changes triggered by salt stress include alterations in the structure and composition of the peptidoglycan layer [ 95 ], and changes in membrane and/or periplasmic protein composition, lipid composition, periplasmic glucan levels, and capsular polysaccharide biosynthesis [ 96 ]. These modifications to the cell wall under salt stress create a diffusion barrier to reduce the influx of inorganic ions [ 97 ]. Salt-stress induced alterations of the peptidoglycan layer involve several enzymes including glycolyl transferases, polysaccharide deacetylases and sugar epimerases.…”
Section: Discussionmentioning
confidence: 99%
“…For bacteria: Bacillus subtilis (current study); the solvent-tolerant Pseudomonas putida 32,[69][70][71] ; the environmentally tenacious Mycobacterium parascrofulaceum and Mycobacterium smegmatis 72 ; and psychrophiles: Glaciecola punicea, Psychrobacter glacincola, Psychrobacter urativorans, and Sporosarcina psychrophilia 73 ; the xerotolerant and acid-tolerant bacterium Gluconacetobacter diazotrophicus 74 and the halotolerant Ochrobactrum sp. 75 . The bacteria that were used in other studies are generally robust, stress-tolerant species, yet B. subtilis (168) exhibited comparable solute-tolerances to many of them.…”
mentioning
confidence: 99%