Role of a Single Aquaporin Isoform in Root Water Uptake

Javot, Hélène; Lauvergeat, Virginie; Santoni, Véronique; Martin-Laurent, Fabrice; Güçlü, Josette; Vinh, Joëlle; Heyes, J.A.; Franck, Katja I.; Schäffner, Anton R.; Bouchez, David; Maurel, Christophe

doi:10.1105/tpc.008888

Cited by 334 publications

(288 citation statements)

References 43 publications

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“…This by no means invalidates the use of pharmacology, but highlights the importance of a diversity of experimental approaches. Future experiments with AQP antisense/ knockout lines (Martre et al, 2002;Javot et al, 2003), particularly for AQPs already shown to mediate NH 3 fluxes (Jahn et al, 2004;Holm et al, 2005;Loqué et al, 2005), could help further elucidate their involvement in futile NH 3 cycling. In addition, such mutant analyses could provide a critical test of the "whole-cell distribution" hypothesis for NH 3 presented above (Fig.…”

Section: Discussionmentioning

confidence: 99%

Rapid Ammonia Gas Transport Accounts for Futile Transmembrane Cycling under NH3/NH4 + Toxicity in Plant Roots

et al. 2013

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Section: Discussionmentioning

confidence: 99%

Rapid Ammonia Gas Transport Accounts for Futile Transmembrane Cycling under NH3/NH4 + Toxicity in Plant Roots

et al. 2013

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“…Aquaporins present in the plasma membrane and also in sthe tonoplast are regulated by environmental factors such as drought, salinity, hormones, and blue light (Guerrero et al, 1990;Yamaguchi-Shinozaki et al, 1992;Yamada et al, 1995). Antisense PIP2 transformed lines resulted in reduced hydraulic conductance (Javot et al, 2003) and alterations in plant morphology (Kaldenhoff et al, 1998) while, overexpression of a plasma membrane aquaporin PIP1b, in transgenic tobacco improved plant vigor under favorable growth conditions but not under drought or salt stress (Aharon et al, 2003). Therefore, approaches involving alterations in plant plasma membrane aquaporins should, however, be viewed with prudence.…”

Section: Protein Complexes Involved In Water Deficit Responsesmentioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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“…Aquaporins, which belong to the highly conserved major intrinsic protein (MIP) family, play an essential role in plant water transport [1][2][3]. Various molecular and cellular mechanisms underlying aquaporin regulation in a challenging environment has been well documented.…”

Section: Introductionmentioning

confidence: 99%

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

et al. 2006

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Aquaporins play a significant role in plant water relations. To further understand the aquaporin function in plants under water stress, the expression of a subgroup of aquaporins, plasma membrane intrinsic proteins (PIPs), was studied at both the protein and mRNA level in upland rice (Oryza sativa L. cv. Zhonghan 3) and lowland rice (Oryza sativa L. cv. Xiushui 63) when they were water stressed by treatment with 20% polyethylene glycol (PEG). Plants responded differently to 20% PEG treatment. Leaf water content of upland rice leaves was reduced rapidly. PIP protein level increased markedly in roots of both types, but only in leaves of upland rice after 10 h of PEG treatment. At the mRNA level, OsPIP1;2, Os-PIP1;3, OsPIP2;1 and OsPIP2;5 in roots as well as OsPIP1;2 and OsPIP1;3 in leaves were significantly up-regulated in upland rice, whereas the corresponding genes remained unchanged or down-regulated in lowland rice. Meanwhile, we observed a significant increase in the endogenous abscisic acid (ABA) level in upland rice but not in lowland rice under water deficit. Treatment with 60 µM ABA enhanced the expression of OsPIP1;2, OsPIP2;5 and OsPIP2;6 in roots and OsPIP1;2, OsPIP2;4 and OsPIP2;6 in leaves of upland rice. The responsiveness of PIP genes to water stress and ABA were different, implying that the regulation of PIP genes involves both ABA-dependent and ABA-independent signaling pathways during water deficit.

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Role of a Single Aquaporin Isoform in Root Water Uptake

Cited by 334 publications

References 43 publications

Rapid Ammonia Gas Transport Accounts for Futile Transmembrane Cycling under NH3/NH4 + Toxicity in Plant Roots

Rapid Ammonia Gas Transport Accounts for Futile Transmembrane Cycling under NH3/NH4 + Toxicity in Plant Roots

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment

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