Role of Acid-Soluble Phosphorus Compounds in Red Blood Cells

Guest, George M.

doi:10.1001/archpedi.1939.01990100154015

Cited by 90 publications

(10 citation statements)

References 19 publications

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“…Several authors have implicated 2,3-DPG metabolism in the maintenance of ionic equilibria in red blood cells. Guest and Rapoport (3) suggested that 2,3-DPG functions as an adjustable pool of impermeant anions which acts to stabilize cell volume in various pathologic states. Gardos (4,5) has postulated that the permeability of the cell membrane to potassium ions depends on the activity within the cell of 2,3-DPG phosphatase, the enzyme which splits 2,3-DPG into 3-PG and Pi.…”

Section: Introductionmentioning

confidence: 99%

Influence of 2,3-diphosphoglycerate metabolism on sodium-potassium permeability in human red blood cells: studies with bisulfite and other redox agents

Parker¹

1969

J. Clin. Invest.

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Section: Introductionmentioning

confidence: 99%

Influence of 2,3-diphosphoglycerate metabolism on sodium-potassium permeability in human red blood cells: studies with bisulfite and other redox agents

Parker¹

1969

J. Clin. Invest.

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“…Similar changes do not occur with the senescence of erythrocytes in vivo (12). The deviations from the normal pattern may be reversed following the transfusion of stored blood (14), and by the addition of some of the purine nucleosides in vitro (17)(18)(19)(20)(21)(22)(23)(24) (31,32).The purpose of this study was to find out if the action of specific antibodies on fetal erythrocytes produces any alteration in the phosphate partitioning. The intra-erythrocytic phosphate pattern of normal fetal red cells was studied and compared with that of the red cells of adults.…”

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confidence: 99%

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confidence: 99%

“…Abnormalities of the metabolism of the erythrocytes in hereditary spherocytosis, auto-immune hemolytic disease with spherocytosis, and in hereditary non-spherocytic hemolytic disease have been demonstrated (25)(26)(27)(28). The literature contains only fragmentary data on partitioning of the acid-soluble phosphorus compounds of fetal erythrocytes (2,(29)(30)(31)(32). Elevated values have been reported for the inorganic and ester phosphates in the red cells of normal infants (31,32).…”

mentioning

confidence: 99%

“…The literature contains only fragmentary data on partitioning of the acid-soluble phosphorus compounds of fetal erythrocytes (2,(29)(30)(31)(32). Elevated values have been reported for the inorganic and ester phosphates in the red cells of normal infants (31,32).…”

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The Phosphate Partition of the Erythrocytes of Normal Newborn Infants and of Infants With Hemolytic Disease

Greenwalt¹,

Ayers²

1956

J. Clin. Invest.

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The mammalian erythrocyte depends on a complex glycolytic mechanism for most of its energy. It is believed that this mechanism controls the transfer of cations across the cell membrane, and serves to maintain the biconcave shape of the red blood cell. Adenosine triphosphate and 2,3-diphosphoglycerate participate in the energy exchanges involved in these processes (1)(2)(3)(4)(5)(6)(7)(8)(9).Alterations in the relationship of the phosphate fractions develop in erythrocytes during storage (10-16). Similar changes do not occur with the senescence of erythrocytes in vivo (12). The deviations from the normal pattern may be reversed following the transfusion of stored blood (14), and by the addition of some of the purine nucleosides in vitro (17)(18)(19)(20)(21)(22)(23)(24) (31,32).The purpose of this study was to find out if the action of specific antibodies on fetal erythrocytes produces any alteration in the phosphate partitioning. The intra-erythrocytic phosphate pattern of normal fetal red cells was studied and compared with that of the red cells of adults. MATERIAL AND METHODSAll the infants studied had been delivered normally but were otherwise unselected. There were ten female and six male babies in the normal group and nine females and one male with erythroblastosis fetalis. The average weight of the normal infants was 7.6 pounds (6.1 to 9.0), and the weight of the infants with hemolytic disease averaged 6.8 pounds (5.1 to 8.4). The blood group of one normal infant was A and that of the mother B.The blood group of two infants with hemolytic disease was AB and their mothers were A2 and B. The other infants were all products of homospecific pregnancies. The medication and anesthesia administered for delivery were similar in the two groups.The cord blood 1 of the normal babies was drawn aseptically from the umbilical vein at the time of delivery. The blood of the infants with hemolytic disease was obtained at the time of the first replacement transfusion within the first eight hours of life. All of the blood specimens were collected into acid-citrate-dextrose solution (ACD) formula A2 in the proportion of 100 ml. of blood to 15 ml. of preservative. The final concentration of glucose added was approximately 320 mg. per cent in whole blood. The specimens reached the laboratory within 30 minutes. Aliquots were distributed into sterile vacuum tubes. The chemical analysis of the fresh specimens was started immediately. The remaining aliquots were stored at 40 to 60 C. Mixing of the specimens during storage was avoided in order to duplicate the usual conditions under which "bank blood" is kept. Each specimen was cultured at the time of use, and the data were discarded if contamination was present.For studying the effect of antibodies added in vitro the plasma was removed from a portion of the blood specimen and replaced with sterile anti-D serum 3 and ACD solution. This mixture and the control specimen were incubated at 37 + 1°C in a water bath, and aliquots of each were distributed into sterile tubes for storage at 4...

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Metabolic Studies in Diabetic Acidosis

Franks¹

1948

Arch Intern Med (Chic)

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Role of Acid-Soluble Phosphorus Compounds in Red Blood Cells

Cited by 90 publications

References 19 publications

Influence of 2,3-diphosphoglycerate metabolism on sodium-potassium permeability in human red blood cells: studies with bisulfite and other redox agents

Influence of 2,3-diphosphoglycerate metabolism on sodium-potassium permeability in human red blood cells: studies with bisulfite and other redox agents

The Phosphate Partition of the Erythrocytes of Normal Newborn Infants and of Infants With Hemolytic Disease

Metabolic Studies in Diabetic Acidosis

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