Role of adipose-derived stem cells in fat grafting and reconstructive surgery

Tan, Shaun; Ng, Zhi Yang; Zhan, Weiqing; Rozen, Warren M.

doi:10.4103/0974-2077.191672

Cited by 33 publications

(22 citation statements)

References 29 publications

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“…Therefore, the addition of HASCs to lipoaspirate may prevent graft volume loss and enhance blood vessel generation in the grafts. This hypothesis has been confirmed in previous clinical trials (4–6). However, the use of autologous HASCs has not been Food and Drug Administration-approved; this may be due to the fact that the reconstructive mechanism of HASCs remains to be fully elucidated.…”

Section: Introductionsupporting

confidence: 81%

An lncRNA‑miRNA‑mRNA ceRNA network for adipocyte differentiation from human adipose‑derived stem cells

Guo

Cao

2019

Mol Med Report

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Human adipose tissue-derived stromal stem cells (HASCs) represent a promising regenerative resource for breast reconstruction and augmentation. However, the mechanisms involved in inducing its adipogenic differentiation remain to be fully elucidated. The present study aimed to comprehensively investigate the expression changes in mRNAs, microRNAs (miRNAs) and long non-coding (lnc)RNAs during the adipogenic differentiation of HASCs, and screen crucial lncRNA-miRNA-mRNA interaction axes using microarray datasets GSE57593, GSE25715 and GSE61302 collected from the Gene Expression Omnibus database. Following pretreatment, differentially expressed genes (DEGs), miRNAs (DE-miRNAs) or lncRNAs (DE-lncRNAs) between undifferentiated and differentiated HASCs were identified using the Linear Models for Microarray data method. A protein-protein interaction (PPI) network was constructed for the DEGs based on protein databases, followed by module analysis. The ‘lncRNA-miRNA-mRNA’ competing endogenous RNA (ceRNA) network was constructed based on the interactions between miRNAs and mRNAs, lncRNAs and miRNAs predicted by the miRWalk and lnCeDB databases. The underlying functions of mRNAs were predicted using the clusterProfiler package. In the present study, 905 DEGs, 36 DE-miRNAs and 577 DE-lncRNAs were screened between undifferentiated HASCs and differentiated adipocyte cells. PPI network analysis demonstrated that LEP may be a hub gene, which was also enriched in significant module 5. LEP was predicted to be involved in the Janus kinase-signal transducer and activator of transcription signaling pathway, and the regulation of inflammatory response. The upregulation of LEP was regulated by downregulated hsa-miRNA (miR)-130b-5p and hsa-miR-23a-5p (or hsa-miR-302d-3p). These miRNAs also respectively interacted with RP11-552F3.9 (or RP11-15A1.7), ultimately forming the ceRNA axes. In conclusion, the present study revealed that the RP11-552F3.9 (RP11-15A1.7)-hsa-miR-130b-5p/hsa-miR-23a-5p (hsa-miR-302d-3p)-LEP interaction axes may be crucial for inducing the adipogenic differentiation of HASCs via involvement in inflammation.

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Section: Introductionsupporting

confidence: 81%

An lncRNA‑miRNA‑mRNA ceRNA network for adipocyte differentiation from human adipose‑derived stem cells

Guo

Cao

2019

Mol Med Report

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“…Mesenchymal stem cells are of particular interest to the field of regenerative medicine due to their wide array of therapeutic benefits [ 31 – 36 ]. The majority of published research has utilized ASCs isolated from either adipose tissue biopsies of abdominal tissue or from lipoaspirate collected during liposuction procedures [ 37 – 39 ]. Human lipoaspirate is also currently used for adipose tissue reconstruction procedure; the reparative effect of lipoaspirate is currently attributed to the ASCs present within the heterogeneous mixture of cells [ 40 – 43 ].…”

Section: Discussionmentioning

confidence: 99%

Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells

Mangum

Natesan

Stone

et al. 2017

Stem Cells International

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Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH). Most (95–99%) cells cultured in FBS were stained positive for CD73, CD90, CD105, and CD142. FBS supplementation was associated with increased triglyceride content and expression of adipogenic genes. Culture in hPL significantly decreased surface staining of CD105 by 31% and 48% and CD142 by 27% and 35% in HAP and BH, respectively (p < 0.05). Culture of BH-ASCs in hPL also increased expression of markers of osteogenesis and increased ALP activity. These data indicate that application of ASCs for wound healing may be influenced by ASC source as well as culture conditions used to expand them. As such, these factors must be taken into consideration before ASCs are used for regenerative purposes.

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“…With the overall survival rates increasing, more and more patients select breast reconstruction as a way to reduce the disabling effects of the mutilation . Autologous fat graft has been used as a filler for breast reconstruction in breast cancer patients following conservative surgery . That is partly because the ADSCs in adipose tissues (about 10 5 ADSCs per gram adipose tissue) can secrete numerous cytokines to benefit the proliferation and angiogenesis of the graft which make it a better graft than other materials .…”

Section: Introductionmentioning

confidence: 99%

Human Adipose-Derived Mesenchymal Stem Cell-Secreted CXCL1 and CXCL8 Facilitate Breast Tumor Growth By Promoting Angiogenesis

Wang¹,

Liu²,

Jiang

et al. 2017

Stem Cells

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Autologous adipose tissue or adipose tissue with additive adipose-derived mesenchymal stem cells (ADSCs) is used in the breast reconstruction of breast cancer patients who undergo mastectomy. ADSCs play an important role in the angiogenesis and adipogenesis, which make it much better than other materials. However, ADSCs may promote residual tumor cells to proliferate or metastasize, and the mechanism is still not fully understood. In this study, we demonstrated that human ADSCs (hADSCs) could facilitate tumor cells growth after co-injection with MCF7 and ZR-75-30 breast cancer cells (BCCs) by promoting angiogenesis, but hADSCs showed limited effect on the growth of MDA-MB-231 BCCs. Intriguingly, compared with ZR-75-30 tumor cells, MCF7 tumor cells were more potentially promoted by hADSCs in the aspects of angiogenesis and proliferation. Consistent with this, cytokine and angiogenesis array analyses showed that after co-injection with hADSCs, the CXCL1 and CXCL8 concentration were significantly increased in MCF7 tumor, but only moderately increased in ZR-75-30 tumor and did not increase in MDA-MB-231 tumor. Furthermore, we found that CXCL1/8 were mainly derived from hADSCs and could increase the migration and tube formation of human umbilical vein endothelial cells (HUVECs) by signaling via their receptors CXCR1 and CXCR2. A CXCR1/2-specific antagonist (SCH527123) attenuated the angiogenesis and tumor growth in vivo. Our findings suggest that CXCL1/8 secreted by hADSCs could promote breast cancer angiogenesis and therefore provide better understanding of safety concerns regarding the clinical application of hADSCs and suggestion in further novel therapeutic options. Stem Cells 2017;35:2060-2070.

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Role of adipose-derived stem cells in fat grafting and reconstructive surgery

Cited by 33 publications

References 29 publications

An lncRNA‑miRNA‑mRNA ceRNA network for adipocyte differentiation from human adipose‑derived stem cells

An lncRNA‑miRNA‑mRNA ceRNA network for adipocyte differentiation from human adipose‑derived stem cells

Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells

Human Adipose-Derived Mesenchymal Stem Cell-Secreted CXCL1 and CXCL8 Facilitate Breast Tumor Growth By Promoting Angiogenesis

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