Role of adipose tissue inflammation in fat pad loss induced by fasting in lean and mildly obese mice

Lacerda, Débora Romualdo; Costa, Kátia Anunciação; Silveira, Ana Letícia Malheiros; Rodrigues, Débora F.; Silva, Albená Nunes; Sabino, Josiana Lopes; Pinho, Vanessa; Menezes, Gustavo B.; Soares, Danusa Dias; Teixeira, Mauro Martins; Ferreira, Adaliene Versiani Matos

doi:10.1016/j.jnutbio.2019.06.006

Cited by 14 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 14 Adipokines, serum glucose and cholesterol, serum and liver triglycerides levels, were measured as described in. 16 Hepatic total fat content was extracted by the Folch method.…”

Section: Methodsmentioning

confidence: 99%

Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background & Aims The precise determination of non-alcoholic fatty liver disease (NAFLD) onset is challenging. Thus, the initial hepatic responses to fat accumulation, which may be fundamental to our understanding of NAFLD evolution and clinical outcomes, are largely unknown. Herein, we chronologically mapped the immunologic and metabolic changes in the liver during the early stages of fatty liver disease in mice and compared this with human NAFLD samples. Methods Liver biopsies from patients with NAFLD (NAFLD activity score [NAS] 2–3) were collected for gene expression profiling. Mice received a high-fat diet for short periods to mimic initial steatosis and the hepatic immune response was investigated using a combination of confocal intravital imaging, gene expression, cell isolation, flow cytometry and bone marrow transplantation assays. Results We observed major immunologic changes in patients with NAS 2–3 and in mice in the initial stages of NAFLD. In mice, these changes significantly increased mortality rates upon drug-induced liver injury, as well as predisposing mice to bacterial infections. Moreover, deletion of Toll-like receptor 4 in liver cells dampened tolerogenesis, particularly in Kupffer cells, in the initial stages of dietary insult. Conclusion The hepatic immune system acts as a sentinel for early and minor changes in hepatic lipid content, mounting a biphasic response upon dietary insult. Priming of liver immune cells by gut-derived Toll-like receptor 4 ligands plays an important role in liver tolerance in initial phases, but continuous exposure to insults may lead to damage and reduced ability to control infections. Lay summary Fatty liver is a very common form of hepatic disease, leading to millions of cases of cirrhosis every year. Patients are often asymptomatic until becoming very sick. Therefore, it is important that we expand our knowledge of the early stages of disease pathogenesis, to enable early diagnosis. Herein, we show that even in the early stages of fatty liver disease, there are significant alterations in genes involved in the inflammatory response, suggesting that the hepatic immune system is disturbed even following minor and undetectable changes in liver fat content. This could have implications for the diagnosis and clinical management of fatty liver disease.

show abstract

“… 14 Adipokines, serum glucose and cholesterol, serum and liver triglycerides levels, were measured as described in. 16 Hepatic total fat content was extracted by the Folch method.…”

Section: Methodsmentioning

confidence: 99%

Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Mao et al reported that 36 h of fasting significantly reduced the amount of white adipose tissue in 24-wk-old male C57BL/6 mice fed a normal diet, and similar results were seen in the same mice fed a high-fat diet (13). On the other hand, Lacerda et al reported that 24 h of fasting in lean male BALB-c mice fed a normal diet resulted in a reduction of adiposity, but this did not occur in mildly obese mice fed a high-carbohydrate refined diet (14). They speculated that the inflammatory response triggered by fasting was impaired in obesity, and therefore, lower degree of adipose tissue loss was observed even after 24 h of fasting.…”

Section: Discussionmentioning

confidence: 56%

The Effects of Overnight Fasting Duration on Glucose and Lipid Metabolism in a Sprague-Dawley Rat Model of Nonalcoholic Steatohepatitis with Advanced Fibrosis

OMAGARI,

MARUTA,

YAYAMA

et al. 2023

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

Nonalcoholic steatohepatitis (NASH) can progress to hepatic fibrosis, and is associated with cardiovascular and liver-related mortality. To understand the pathogenesis of NASH, reliable animal models of the disease are useful. In animal studies, the animals are usually fasted overnight before biospecimens are taken, but little is known about the effects of fasting. Here, we investigated the impact of overnight fasting for approximately 9 to 17 h on glucose and lipid metabolism in a Sprague-Dawley (SD) rat model of diet-induced moderate and advanced NASH in comparison to normal SD rats. Our results revealed that in the moderate NASH model rats, the fasting duration did not affect glucose and lipid metabolism, the histopathological findings, or the hepatic mRNA expression levels of genes related to lipid metabolism, cholesterol metabolism, inflammation, fibrosis, and oxidative stress. In contrast, in the normal rats, significant fasting time-dependent reductions were observed in the epididymal fat pad weight and the hepatic mRNA expression levels of adipose differentiation-related protein and heme oxygenase-1. Moreover, in the advanced NASH model rats, a significant fasting time-dependent reduction and increase were observed in the serum insulin level and mRNA expression level of alpha-smooth muscle actin, respectively. Our present results suggest that the influence of the overnight fasting duration differs among the healthy condition, moderate NASH, and advanced NASH statuses. Further studies are needed in humans to determine the appropriate overnight fasting duration for the accurate evaluation of glucose and lipid metabolism in NASH patients.

show abstract

Reappraisal of Adipose Tissue Inflammation in Obesity

Engin

2024

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Role of adipose tissue inflammation in fat pad loss induced by fasting in lean and mildly obese mice

Cited by 14 publications

References 38 publications

Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD

Imaging and immunometabolic phenotyping uncover changes in the hepatic immune response in the early phases of NAFLD

The Effects of Overnight Fasting Duration on Glucose and Lipid Metabolism in a Sprague-Dawley Rat Model of Nonalcoholic Steatohepatitis with Advanced Fibrosis

Reappraisal of Adipose Tissue Inflammation in Obesity

Contact Info

Product

Resources

About