Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress

Wu, Yujing; Song, Siyuan; Liu, He; Xing, Dan; Wang, Xin; Yan, Fei; Li, Guangling; Zhang, Chao; Liu, Ying; Zhang, Licai

doi:10.1016/j.npep.2015.03.007

Cited by 16 publications

(6 citation statements)

References 45 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and neuron–body fluids pathways. Moreover, in previous studies, the distribution and change in the expression of substance P (Lu et al, 2011), acid-sensing ion channel 3 (ASIC 3 ) (Wang et al, 2014), transient receptor potential vanniloid-1 (TRPV 1 ) (Xu et al, 2011), Wnt 5a (Wang J. et al, 2015), extracellular signal-regulated kinase-5 (ERK 5 ) (Wang C. G. et al, 2015), mechanistic target of rapamycin (mTOR) and ERK 1/2 (Li et al, 2015), neurokinin-1 receptor (NK 1 R) (Zhang et al, 2017), and adrenomedullin (Wu et al, 2015), as well as dozens of neuroactive substances (including neurotransmitters, receptors, and ion channel proteins) in the CSF-contacting nucleus in morphine physical dependence (Lu et al, 2011), inflammatory pain (Wang et al, 2014), neuropathic pain (Xu et al, 2011; Li et al, 2015; Wang C. G. et al, 2015; Wang J. et al, 2015), visceral pain (Zhang et al, 2017) and stress (Wu et al, 2015). Studying the characteristics of the CSF-contacting nucleus and understanding the changes in the substances distributed in the nucleus will help elucidate the function of the nucleus and its relationship to physiological processes.…”

Section: Discussionmentioning

confidence: 99%

Stereotaxic Coordinates and Morphological Characterization of a Unique Nucleus (CSF-Contacting Nucleus) in Rat

Song

Bao

et al. 2019

Front. Neuroanat.

Self Cite

View full text Add to dashboard Cite

A unique nucleus, the cerebrospinal fluid (CSF)-contacting nucleus, has recently been recognized in the brain parenchyma. The outstanding feature of this nucleus is that the neural somas are located in the parenchyma, but their processes stretch into the CSF, implying that it may be a key structure bridging the nervous and body fluids-regulating systems and may play a pivotal role in modulating physiological activities. However, the true biological significance of this nucleus needs to be uncovered. The morphology of a nucleus is one of the most important parameters for neuroscience studies. For this reason, a common experimental animal, Sprague-Dawley (SD) rats, was chosen. The position, adjacent structures, neuronal distribution, size, three-dimensional reconstruction, and core coordinates of the CSF-contacting nucleus in SD rats of different weights (90–400 g) were illustrated for the first time. Furthermore, the formulas for calculating the core coordinates of the CSF-contacting nucleus in rats of different weights were revealed. Finally, the possible biological functions uncovered by past research are reviewed in this paper. This study provides an indispensable methodology and a significant reference for researchers interested in this unique nucleus.

show abstract

Section: Discussionmentioning

confidence: 99%

Stereotaxic Coordinates and Morphological Characterization of a Unique Nucleus (CSF-Contacting Nucleus) in Rat

Song

Bao

et al. 2019

Front. Neuroanat.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, our recent studies reveal that this nucleus receives the projections from the hypothalamus (Song et al, 2020b) and brainstem (Song et al, 2020a), which implies that the CSF-contacting nucleus participates in complex and diverse neural circuits modulating different behaviors. The relationships with some of the life activities such as pain (Liu et al, 2017), stress (Wu et al, 2015), and drug addiction (Lu et al, 2011) have been already reported.…”

Section: Introductionmentioning

confidence: 96%

Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objective: To identify the novel projections received by the cerebrospinal fluid (CSF)contacting nucleus from the subcortex and limbic system to understand the biological functions of the nucleus. Methods: The cholera toxin subunit B (CB), a retrograde tracer, was injected into the CSF-contacting nucleus in Sprague-Dawley rats. After 7-10 days, the surviving rats were perfused, and the whole brain and spinal cord were sliced for CB immunofluorescence detection. The CB-positive neurons in the subcortex and limbic system were observed under a fluorescence microscope, followed by 3D reconstructed with the imaris software. Results: CB-positive neurons were found in the basal forebrain, septum, periventricular organs, preoptic area, and amygdaloid structures. Five functional areas including 46 sub-regions sent projections to the CSF-contacting nucleus. However, the projections had different densities, ranging from sparse to moderate, to dense. Conclusions: According to the projections from the subcortex and limbic system, we hypothesize that the CSF-contacting nucleus participates in emotion, cognition, homeostasis regulation, visceral activity, pain, and addiction. In this study, we illustrate the novel projections from the subcortex and limbic system to the CSF-contacting nucleus, which underlies the diverse and complicated circuits of the nucleus in body regulations.

show abstract

“…8 This connection is not found in any known nerve or nucleus in the nervous system. The basic biological properties of the CSF-contacting nucleus, such as methods that can be used to specifically label it, 9 its location and stereotactic coordinates, 8 the distribution of receptors, neurotransmitters, and ion channels [10][11][12] and its relationship with morphine dependence and withdrawal, stress, sodium appetite, ion channels 10,[13][14][15] have been revealed. A model animal with the CSF-contacting nucleus eliminated has been successfully established.…”

Section: Introductionmentioning

confidence: 99%

TRPV1 and GABAB1 in the Cerebrospinal Fluid-Contacting Nucleus are Jointly Involved in Chronic Inflammatory Pain in Rats

Xu¹,

Yan²,

Yuan³

et al. 2022

JPR

Self Cite

View full text Add to dashboard Cite

Objective To assess the receptors of TRPV1 and GABA B1 receptors that were colocalized in cerebrospinal fluid contacting nucleus (CSF-contact nucleus) of chronic inflammatory pain (CIP) rats bringing inspiration for reducing chronic pain. Methods A rat model of CIP was constructed by plantar injection of complete Freund’s adjuvant (CFA), and the paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured 1, 3, 5, 7, 10, and 14 days after plantar injection. In the first part of the experiment, rats with CIP were divided into the immunofluorescence group and the coimmunoprecipitation (Co-IP) group (n = 6). Rats in the immunofluorescence group were injected with the retrograde tracer CB conjugated with Alexa Fluor 594 into the lateral ventricle two days before the injection of CFA into the plantar surface of the left paw. Three days later, rats that exhibited hyperalgesia were perfused, and their brains were extracted and used for double immunofluorescence staining of the CSF-contacting nucleus. Rats in the Co-IP group were anesthetized and dissected 3 days after CFA injection, and fresh brain segments containing the CSF-contacting nucleus were collected for Co-IP to assess the colocalization of TRPV1 and GABA B1 in the CSF-contacting nucleus (n = 6). In the second part of the experiment, SD rats were divided into the normal saline group (control group) and the CFA group. Fresh CSF-contacting nucleus-containing tissues were collected for Western blot analysis 3 days after plantar injection to observe the changes in TRPV1 and GABA B1 expression in the CSF-contacting nucleus. Results TRPV1 and GABA B1 were co-expressed in the CSF-contacting nucleus in rats with CIP, and their expression was upregulated. Conclusion TRPV1 and GABA B1 in the CSF-contacting nucleus are jointly involved in CIP in rats, and there is a direct or indirect link between TRPV1 and GABA B1 .

show abstract

Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress

Cited by 16 publications

References 45 publications

Stereotaxic Coordinates and Morphological Characterization of a Unique Nucleus (CSF-Contacting Nucleus) in Rat

Stereotaxic Coordinates and Morphological Characterization of a Unique Nucleus (CSF-Contacting Nucleus) in Rat

Novel Projections to the Cerebrospinal Fluid-Contacting Nucleus From the Subcortex and Limbic System in Rat

TRPV1 and GABAB1 in the Cerebrospinal Fluid-Contacting Nucleus are Jointly Involved in Chronic Inflammatory Pain in Rats

Contact Info

Product

Resources

About