Role of ALK5/Smad2/3 and MEK1/ERK Signaling in Transforming Growth Factor Beta 1–modulated Growth, Collagen Turnover, and Differentiation of Stem Cells from Apical Papilla of Human Tooth

Chang, Hung‐Hao; Chang, Mei‐Chi; Wu, I-Hua; Huang, Guan‐Tarn; Huang, Weiling; Wang, Yin‐Lin; Lee, Sheng-Yang; Yeh, Chi‐Chuan; Guo, Ming-Kuang; Chan, Chiu‐Po; Hsien, Hsiang-Chi; Jeng, Jiiang‐Huei

doi:10.1016/j.joen.2015.03.022

Cited by 45 publications

(36 citation statements)

References 53 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MAPK/ERK signaling is an important pathway that affects cell proliferation, and can be activated by growth factors such as TGF-β1 [26], [27], [28]. Because our data showed that GTP induced the expression of TGF-β1, we therefore examined the effect of GTP on ERK signaling pathway by Western blotting and immunofluorescence staining.…”

Section: Resultsmentioning

confidence: 99%

Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway

Chen

Wang

et al. 2017

Journal of Ginseng Research

View full text Add to dashboard Cite

BackgroundRecently, protein from ginseng was studied and used for the treatment of several kinds of diseases. However, the effect of ginseng total protein (GTP) on proliferation and wound healing in fibroblast cells remains unclear.MethodsIn this study, cell viability was analyzed using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Cell cycle distribution was analyzed by flow cytometer. The levels of transforming growth factor β1, vascular endothelial growth factor, and collagens were analyzed by enzyme-linked immunosorbent assay and immunofluorescence staining. The expressions of cyclin A, phosphorylation of extracellular signal-related kinase (p-ERK1/2), and ERK1/2 were analyzed by Western blotting.ResultsOur results showed that GTP promoted cell proliferation and increased the percentage of cells in S phase through the upregulation of cyclin A in NIH/3T3 cells. We also found that GTP induced the secretion of type I collagen, and promoted the expression of other factors that regulate the synthesis of collagen such as transforming growth factor β1 and vascular endothelial growth factor. In addition, the phosphorylation of ERK1/2 at Thr202/Tyr204 was also increased by GTP.ConclusionOur studies suggest that GTP promoted proliferation and secretion of collagen in NIH/3T3 cells by activating the ERK signal pathway, which shed light on a potential function of GTP in promoting wound healing.

show abstract

Section: Resultsmentioning

confidence: 99%

Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway

Chen

Wang

et al. 2017

Journal of Ginseng Research

View full text Add to dashboard Cite

show abstract

“…In a recent study, Plasminogen activator Inhibitor 1 (PAI-1), has been suggested as pivotal factor in inducing odontogenic differentiation of SCAP [107]. Finally, a number of studies have also closely studied the signaling pathways regulating odontogenic differentiation of SCAP; among these, differential activation of ALK5/Smad2 and MEK/ERK [108], canonical Wnt synergistic with BMP-9 [109], Notch [110], canonical NF- κ B [111], and ERK and JNK signaling pathways in combination with a mechanical stress stimulus have been indicated as having a paramount role in the committed differentiation of SCAP [112]. …”

Section: Differentiation Potential and Paracrine Activity Of Dentamentioning

confidence: 99%

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Bakopoulou

About

2016

Stem Cells International

View full text Add to dashboard Cite

Dental Mesenchymal Stem Cells (MSCs), including Dental Pulp Stem Cells (DPSCs), Stem Cells from Human Exfoliated Deciduous teeth (SHED), and Stem Cells From Apical Papilla (SCAP), have been extensively studied using highly sophisticated in vitro and in vivo systems, yielding substantially improved understanding of their intriguing biological properties. Their capacity to reconstitute various dental and nondental tissues and the inherent angiogenic, neurogenic, and immunomodulatory properties of their secretome have been a subject of meticulous and costly research by various groups over the past decade. Key milestone achievements have exemplified their clinical utility in Regenerative Dentistry, as surrogate therapeutic modules for conventional biomaterial-based approaches, offering regeneration of damaged oral tissues instead of simply “filling the gaps.” Thus, the essential next step to validate these immense advances is the implementation of well-designed clinical trials paving the way for exploiting these fascinating research achievements for patient well-being: the ultimate aim of this ground breaking technology. This review paper presents a concise overview of the major biological properties of the human dental MSCs, critical for the translational pathway “from bench to clinic.”

show abstract

“…Some studies suggest that TGF-β1 can inhibit the proliferation of SCAPs and their mineralization by decreasing the osteogenic/dentinogenic gene expressions [ 59 ]. In detail, TGF-β1 promotes the cell growth, collagen content and ALP activity at lower concentrations (0.1-1 ng/mL) but down-regulates the activity at higher concentrations (>5 ng/mL) by regu lating ERK1/2 and Smad2 signaling pathways [ 60 ].…”

Section: Tgf-β Signaling Pathwaymentioning

confidence: 99%

Signaling Pathways in Dental Stem Cells During Their Maintenance and Differentiation

Liu

Zhou

et al. 2016

Stem Cell Biology and Regenerative Medicine

View full text Add to dashboard Cite

Role of ALK5/Smad2/3 and MEK1/ERK Signaling in Transforming Growth Factor Beta 1–modulated Growth, Collagen Turnover, and Differentiation of Stem Cells from Apical Papilla of Human Tooth

Cited by 45 publications

References 53 publications

Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway

Panax ginseng total protein promotes proliferation and secretion of collagen in NIH/3T3 cells by activating extracellular signal-related kinase pathway

Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

Signaling Pathways in Dental Stem Cells During Their Maintenance and Differentiation

Contact Info

Product

Resources

About