Role of Anionic Charges of Periplasmic Glucans of<i>Shigella flexneri</i>in Overcoming Detergent Stress

Bhagwat, Arvind A.; Leow, Yi Ning; Liu, Liu; Dharne, Mahesh; Porteen, K.

doi:10.1089/fpd.2011.1090

Cited by 6 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phosphate substitution on the C. lari fOS might aid in increasing the net negative charge in the periplasmic space preventing charged antimicrobials from entering the cell or to retain the fOS in the periplasm to fulfill a similar function in osmo‐adaptation in C. lari as has been shown for C. jejuni fOS . In other proteobacteria, OPGs can be substituted with a variety of non‐carbohydrate components dependent on the media or growth phase and have been shown to increase the resistance to anionic detergents in Shigella flexneri . In Brucella species , cyclic periplasmic glucans are potent activators of human and mouse dendritic cells and increase IL‐6 production .…”

Section: Discussionmentioning

confidence: 97%

“…Both fOS and OPG concentrations decrease with increasing extracellular osmolarity (proposed mechanism in Figure ) . OPGs play crucial roles in osmotic adaptation, motility, antibiotic resistance, intercellular signaling, pathogenesis, and plant symbiosis . In C. jejuni , mutants in the pgl pathway display several altered phenotypes such as reduced attachment and invasion of human epithelial cells, impaired colonization of the intestinal tracts of mice and chickens, reduced natural competence in strains with Type IV secretion pathways and reduced binding by the human macrophage C‐type lectin, MGL .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Generation of Free Oligosaccharides from Bacterial Protein N‐Linked Glycosylation Systems

et al. 2013

View full text Add to dashboard Cite

All Campylobacter species are capable of N-glycosylating their proteins and releasing the same oligosaccharides into the periplasm as free oligosaccharides (fOS). Previously, analysis of fOS production in Campylobacter required fOS derivatization or large culture volumes and several chromatography steps prior to fOS analysis. In this study, label-free fOS extraction and purification methods were developed and coupled with quantitative analysis techniques. Our method follows three simple steps: (1) fOS extraction from the periplasmic space, (2) fOS purification using silica gel chromatography followed by porous graphitized carbon purification and (3) fOS analysis and accurate quantitation using a combination of thin-layer chromatography, mass spectrometry, NMR, and high performance anion exchange chromatography with pulsed amperometric detection. We applied our techniques to analyze fOS from C. jejuni, C. lari, C. rectus, and C. fetus fetus that produce different fOS structures. We accurately quantified fOS in Campylobacter species that ranged from 7.80 (±0.84) to 49.82 (±0.46) nmoles per gram of wet cell pellet and determined that the C. jejuni fOS comprises 2.5% of the dry cell weight. In addition, a novel di-phosphorylated fOS species was identified in C. lari. This method provides a sensitive and quantitative method to investigate the genesis, biology and breakdown of fOS in the bacterial N-glycosylation systems.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Generation of Free Oligosaccharides from Bacterial Protein N‐Linked Glycosylation Systems

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Amongst them, 16 strains were in Pseudomonas 7 strains , Acinetobacter 6 strains , Acidovorax 1 strain , Aeromonas 1 strain or Shewanella 1 strain , and 1 strain was closest to the uncultured bacterium clone L8. Namely, only 1 strain identified as Klebsiella oxytoca LAS Rajagopal et al, 2003;Bhagwat et al, 2012 . Therefore, the ability to modify OPG may be an important property common to the LAS-resistant bacteria isolated.…”

Section: Figmentioning

confidence: 99%

Antibacterial Activities of Surfactants in the Laundry Detergents and Isolation of the Surfactant Resistant Aquatic Bacteria

Maehara

Miyoshi

2017

Biocontrol Sci.

View full text Add to dashboard Cite

Linear alkylbenzene sulfonate LAS and polyoxyethylene lauryl ether POLE are major surfactants contained in the laundry detergents. In the present study, the antibacterial activities of the surfactants to aquatic microorganisms were compared. When freshwater samples from a small river in Okayama city were treated with each of the surfactants, only LAS showed the significant antibacterial activity. Several strains, which survived after the treatment with 2.0% LAS, were isolated and identified by sequencing of 16S rDNA. All strains were classified into the family Enterobacteriaceae. However, this family was not a major member of the aquatic microflora, suggesting that the bacteria in Enterobacteriaceae have a common property of LAS-resistance in the river water.

show abstract

“…SDS is shown to be toxic to aquatic animals [14] and also to other animals and humans who consume water contaminated with detergents [15]. It is also toxic to microbes [16]. In aquatic systems, it has the tendency to adsorb to surfaces of sediments [17].…”

Section: Introductionmentioning

confidence: 99%

Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate

Chaturvedi

Kumar

2013

Indian J Microbiol

View full text Add to dashboard Cite

Sodium dodecyl sulfate (SDS) is one of the most commonly used detergent, which exhibits excellent biocidal activity against various bacteria and fungi. It is commonly employed in many detergent formulations and is employed for disinfection purposes. It is shown to be toxic to fishes, aquatic animals and is also inhibitory to microbes and cyanobacteria. We had isolated a strain belonging to Pseudomonas aeruginosa N1, from a detergent contaminated pond situated in Varanasi city India, which was able to degrade and metabolize SDS as a source of carbon. In the present investigation, we have studied chemotactic response of this strain towards SDS. The results clearly indicate that this strain showed chemotactic response towards SDS. The nature of chemotaxis was found to be metabolism dependent as glucose grown cells showed a delayed chemotactic response towards SDS. This is first study that reported chemotaxis response for P. aeruginosa towards anionic detergent SDS.

show abstract

Role of Anionic Charges of Periplasmic Glucans ofShigella flexneriin Overcoming Detergent Stress

Cited by 6 publications

References 28 publications

Generation of Free Oligosaccharides from Bacterial Protein N‐Linked Glycosylation Systems

Generation of Free Oligosaccharides from Bacterial Protein N‐Linked Glycosylation Systems

Antibacterial Activities of Surfactants in the Laundry Detergents and Isolation of the Surfactant Resistant Aquatic Bacteria

Metabolism Dependent Chemotaxis of Pseudomonas aeruginosa N1 Towards Anionic Detergent Sodium Dodecyl Sulfate

Contact Info

Product

Resources

About