Role of Annexin A1 in Squamous Cell Lung Cancer Progression

Abstract

Lung cancer remains the primary cause of cancer-related death worldwide, and its molecular mechanisms of tumor progression need further characterization to improve the clinical management of affected patients. The role of Annexin A1 (ANXA1) in tumorigenesis and cancer progression in general and especially in lung cancer remains to be controversial and seems to be highly tissue specific and inconsistent among tumor initiation, progression, and metastasis. In the current study, we investigated ANXA1 expression in 81 squamous cell lung cancer (SQCLC), 86 pulmonary adenocarcinoma (AC), and 30 small cell lung cancer (SCLC) patient-derived tissue samples and its prognostic impact on patient’s survival. Mechanistically, we analyzed the impact of ANXA1 expression on proliferation and migration of SQCLC cell lines using CRISPR-Cas9 and mammalian overexpression vectors. Strong expression of ANXA1 was significantly correlated to longer overall survival only in SQCLC patients ( P = 0.019 ). Overexpression of ANXA1 promoted proliferation in SQCLC cell lines but suppressed their migration, while knockout of ANXA1 promoted cell migration and suppressed proliferation. In conclusion, ANXA1 expression might elongate patients’ survival by inhibiting tumor cell migration and subsequent metastasis.

Harmine loaded Au@MSNs@PEG@Asp6 nano-composites for treatment of spinal metastasis from lung adenocarcinoma by targeting ANXA9 in vivo experiment

“…In line with our bioinformatics findings, ANXA9 knockdown inhibited, and its overexpression promoted, the invasion and migration of A549 cells. This is consistent with previous observations concerning ANXA9 ( 13 ) and other ANXA proteins in different cancers, including lung cancer ( 29 , 30 ). Specifically, it has been well documented that ANXA3 regulates epithelial mesenchymal transition (EMT) and lymph node metastasis in pancreatic cancer through the PI3K/AKT signaling pathway ( 31 ) and ANXA11 promotes invasion, migration, and EMT progress in hepatocellular carcinoma via the activation of AKT signaling ( 32 ).…”

Data‐independent acquisition and quantification of extracellular matrix from human lung in chronic inflammation‐associated carcinomas

“…Additionally, annexin A1, up‐regulated by a factor 4.06 in “Tumor” vs. “Matched Normal” with q ‐value = 5.27e‐7 (Figure 3D ), is a member of the Ca 2+ ‐regulated phospholipid‐binding protein superfamily, involved in various cellular processes, such as inflammation, proliferation regulation, apoptosis, and tumorigenesis [ 53 ]. Notably, Annexin A1 appears as prognostic factor for longer overall survival in LSCC by suppressing metastasis, but not cancer cell proliferation [ 54 ].…”

Role of annexin A1 in early diagnosis of lung cancer

“…The current study included 20 cases with lung cancer lesions (Malignant group) in addition to 19 cases with non-malignant lung diseases. Table (1) shows that there was no statistically difference between the malignancy and the nonmalignant group regarding the age and gender. The highest percentage of the malignant group were males (75%).…”

“…According to the 2020 World Cancer Report, lung cancer continues to cause the grand majority of cancer-related mortality (18%) [1] . Approximately two million new cases are diagnosed each year worldwide [2] .…”