Role of antibody and complement in opsonization of group B streptococci

Shigeoka, Ann O.; Hall, R T; Hemming, Val G.; Allred, Craig; Hill, Harry R.

doi:10.1128/iai.21.1.34-40.1978

Cited by 70 publications

(31 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although concise recommendations for screening procedures and prophylactic treatment of pregnant women with vaginal group B streptococcal (GBS) colonization exist, GBS infections are still a cause of considerable neonatal mortality and morbidity (1). Lack of typespecific opsonic antibodies seems to be one of the main reasons for the susceptibility of premature neonates to early onset GBS septicaemia and pneumonia (2,3). As neonatal GBS infections and respiratory distress syndrome due to surfactant deficiency may occur simultaneously (4), some infants with GBS infections may receive surfactant treatment (5).…”

mentioning

confidence: 99%

Effect of surfactant on nitroblue tetrazolium reduction of polymorphonuclear leucocytes stimulated with type la group B streptococci

et al. 1995

View full text Add to dashboard Cite

Activation of polymorphonuclear leucocytes (PMN) was investigated after incubation of adult human PMN and group B streptococci (GBS) type Ia with a type-specific polyclonal antiserum and a modified porcine surfactant (Curosurf). The level of oxidative metabolism of PMN was studied using a micromethod modification of the nitroblue tetrazolium (NBT) reduction test. GBS alone did not stimulate significant oxygen metabolite release from PMN, and incubation of PMN with surfactant alone resulted in decreased NBT reduction. After opsonization of GBS with a specific antibody, PMN were activated and the increased oxygen metabolite release was not suppressed when surfactant was added to the system. We conclude that the encapsulated GBS strain investigated needs opsonization with specific antibody to increase oxidative metabolism of PMN, and that incubation of PMN and opsonized GBS with surfactant does not interfere with NBT reduction.

show abstract

mentioning

confidence: 99%

Effect of surfactant on nitroblue tetrazolium reduction of polymorphonuclear leucocytes stimulated with type la group B streptococci

et al. 1995

View full text Add to dashboard Cite

show abstract

“…It has been shown that antibody is essential for phagocytosis of GBS by human and bovine PMN [14][15][16][17]. In these studies, phagocytic uptake was assayed using PMN and bacteria maintained in suspension in a fluid medium.…”

Section: Discussionmentioning

confidence: 99%

Involvement of locomotion of granulocytes in the phagocytosis of glass-adherent group B streptococci in the absence of opsonins

Rainard

1988

FEMS Microbiology Letters

View full text Add to dashboard Cite

Phagocytosis of glass‐adherent group B streptococci (GBS) by bovine polymorphonuclear leukocytes (PMN) was investigated by a fluorochrome microassay. Three out of the four tested strains were taken up in the absence of serum, but phagocytosis was increased when immobilized bacteria were pre‐treated by either normal bovine serum (NBS, containing antibodies to GBS), precolostral calf serum (PCS, virtually devoid of antibodies), heat‐inactivated PCS (H‐PCS), or to a lesser extent by human serumalbumin (HSA) or gelatin. The fourth strain required opsonization by NBS to be ingested. The under‐agarose PMN migration assay showed that HSA, PCS, H‐PCS, and NBS had locomotion‐promoting effects that ranked as for enhancement of phagocytosis. In addition, fixed antigen‐antibody complexes inhibited both migration under agarose and surface phagocytosis. These findings suggested that when bacteria are sensitive to surface phagocytosis in the absence of opsonins, the enhancing effect of serum is essentially mediated through promotion of PMN locomotion and not through opsonin‐enhanced ingestion.

show abstract

“…However, the bacteria could be opsonized at a slow rate (30 min, 37°C) through the alternative pathway in the absence of classical pathway activation. Considering the kinetics of the reaction, the opsonic activity in un-requirement of specific antibody for opsonization of serotype 111 (1, 2, 10, 13,16,24) and other serotypes (3,4,8,24).…”

Section: Discussionmentioning

confidence: 99%

“…The role of the alternative pathway in the opsonization of type III is controversial. According to Shigeoka et al (24) opsonization was mediated entirely via the classical pathway while Edwards et al (12) have found that type specific antibodies facilitate the alternative pathway-mediated opsonophagocytosis of serotype 111. In the present study, the alternative pathway appeared to play a role in opsonization of the serotype IIIR-, but not of the IIIR+ strain.…”

Section: Discussionmentioning

confidence: 99%

“…Controversy exists concerning the precise mechanism of complement activation by different serotypes (12). Classical pathway activation, with or without participation of antibody, appears to be required for efficient opsonization of the serotypes Ia and I1 (12,24). In the presence of antibody to capsular sialic acid residues, the alternative pathway could play a crucial role in opsonizatioii of serotype I11 ( 1 1, 13).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Opsonization of Group B Streptococci in Properdin Deficient Serum

Söderström

Braconier

Christensen

et al. 1985

Acta Pathologica Microbiologica Scandinavica Series C: Immunolo

View full text Add to dashboard Cite

Phagocytic killing of group B streptococci serotypes Ia, Ib, IIR‐ (R protein negative), IIR+ (R protein positive), IIIR‐ and IIIR+ by human granulocytes was studied after opsonization in properdin deficient serum, pooled normal human serum and in selected sera with high or low concentrations of antibody to group B streptococci. All serotypes were killed by granulocytes after opsonization in normal serum, but serotype IIIR‐ was comparatively resistant. Properdin deficient serum showed no opsonic activity for type IIIR‐. A reduced opsonic capacity of properdin deficient serum for serotypes Ib and IIR+ was demonstrated, whereas the other serotypes were efficiently opsonized. The reduced or absent opsonic activity of properdin deficient serum could be restored by addition of purified properdin. Antibody levels did not appear to be limiting in the assay system. Blocking of C1 activation with MgEGTA in normal serum delayed, but did not abolish opsonization of the various group B streptococcal serotypes, while the opsonic activity in chelated properdin deficient serum was markedly reduced. Taken together, the findings suggest that an intact classical pathway is crucial in group B streptococcal opsonization. However, efficient opsonization of some strains apparently requires that C3 activation on the bacterial surface is amplified through recruitment of the alternative pathway.

show abstract

Role of antibody and complement in opsonization of group B streptococci

Cited by 70 publications

References 18 publications

Effect of surfactant on nitroblue tetrazolium reduction of polymorphonuclear leucocytes stimulated with type la group B streptococci

Effect of surfactant on nitroblue tetrazolium reduction of polymorphonuclear leucocytes stimulated with type la group B streptococci

Involvement of locomotion of granulocytes in the phagocytosis of glass-adherent group B streptococci in the absence of opsonins

Opsonization of Group B Streptococci in Properdin Deficient Serum

Contact Info

Product

Resources

About