Role of aquaporins in determining transpiration and photosynthesis in water‐stressed plants: crop water‐use efficiency, growth and yield

Moshelion, Menachem; Halperin, О. І.; Wallach, Rony; Oren, Ram; Way, Danielle A.

doi:10.1111/pce.12410

Cited by 203 publications

(156 citation statements)

References 106 publications

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“…Isohydric species reduce stomatal conductance as soil water decreases and the demand for water in the atmosphere increases during drought conditions, maintaining relatively constant midday leaf water potential. In contrast, anisohydric species allow midday leaf water potential to decline as soil dries during drought, thus maintaining stomatal conductance and CO 2 assimilation [10,58,59]. Trees experiencing long-term, persistent drought in our study did not maintain leaf water potentials or reduce stomatal conductance, which could indicate an anisohydric response strategy to long-term drought conditions.…”

Section: Discussioncontrasting

confidence: 61%

Response of Mid-Rotation Loblolly Pine (Pinus taeda L.) Physiology and Productivity to Sustained, Moderate Drought on the Western Edge of the Range

Maggard

Will

Wilson

et al. 2016

Forests

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Abstract:The productivity of the approximately 11 million ha of loblolly pine plantations in the southeastern USA could be threatened by decreased water availability in a future climate. To determine the effects of sustained drought on leaf gas exchange, whole-tree water use, and individual tree growth, we examined the response of loblolly pine trees to 100% throughfall exclusion cumulatively spanning the sixth and seventh growing seasons of a plantation in southeastern Oklahoma. Throughfall exclusion reduced volumetric soil water content for 0-12 cm soil depth from 10.8% to 4.8% and for 12-45 cm soil depth from 24.2% to 15.6%. Compared to ambient throughfall trees, leaf water potential of the throughfall exclusion trees became more negative, −0.9 MPa vs. −1.3 MPa for predawn measurements and −1.5 MPa vs. −1.9 MPa for midday measurements. Throughfall exclusion did not significantly reduce leaf gas exchange or tree water use. However, throughfall exclusion significantly reduced leaf biomass by 21% and stem volume growth by 23%. These results indicate that sustained drought may cause downward shifts in leaf quantity to conserve water rather than reducing leaf-level water use.

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Section: Discussioncontrasting

confidence: 61%

Response of Mid-Rotation Loblolly Pine (Pinus taeda L.) Physiology and Productivity to Sustained, Moderate Drought on the Western Edge of the Range

Maggard

Will

Wilson

et al. 2016

Forests

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“…Indeed, recent studies have suggested that cell shrinkage with dehydration, and/or deactivation of membrane aquaporins outside the xylem, could strongly reduce K leaf (Kim and Steudle, 2007;Shatil-Cohen et al, 2011;Pantin et al, 2013;Scoffoni et al, 2014;Moshelion et al, 2015;Sade et al, 2015). Yet, the vulnerability of K x and K ox , and their influences on K leaf decline with dehydration, have not been clearly disentangled.…”

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confidence: 99%

Outside-Xylem Vulnerability, Not Xylem Embolism, Controls Leaf Hydraulic Decline during Dehydration

et al. 2017

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Leaf hydraulic supply is crucial to maintaining open stomata for CO 2 capture and plant growth. During drought-induced dehydration, the leaf hydraulic conductance (K leaf ) declines, which contributes to stomatal closure and, eventually, to leaf death. Previous studies have tended to attribute the decline of K leaf to embolism in the leaf vein xylem. We visualized at high resolution and quantified experimentally the hydraulic vulnerability of xylem and outside-xylem pathways and modeled their respective influences on plant water transport. Evidence from all approaches indicated that the decline of K leaf during dehydration arose first and foremost due to the vulnerability of outside-xylem tissues. In vivo x-ray microcomputed tomography of dehydrating leaves of four diverse angiosperm species showed that, at the turgor loss point, only small fractions of leaf vein xylem conduits were embolized, and substantial xylem embolism arose only under severe dehydration. Experiments on an expanded set of eight angiosperm species showed that outside-xylem hydraulic vulnerability explained 75% to 100% of K leaf decline across the range of dehydration from mild water stress to beyond turgor loss point. Spatially explicit modeling of leaf water transport pointed to a role for reduced membrane conductivity consistent with published data for cells and tissues. Plant-scale modeling suggested that outside-xylem hydraulic vulnerability can protect the xylem from tensions that would induce embolism and disruption of water transport under mild to moderate soil and atmospheric droughts. These findings pinpoint outside-xylem tissues as a central locus for the control of leaf and plant water transport during progressive drought.

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“…aquaporins [AQPs]; for review, see Moshelion et al, 2014). AQPs are considered to be key channel proteins for the transport of water, small and uncharged solutes, and CO 2 through plant cell membranes (Tyerman et al, 2002;Maurel et al, 2008).…”

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confidence: 99%

The Role of Plasma Membrane Aquaporins in Regulating the Bundle Sheath-Mesophyll Continuum and Leaf Hydraulics

et al. 2014

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Our understanding of the cellular role of aquaporins (AQPs) in the regulation of whole-plant hydraulics, in general, and extravascular, radial hydraulic conductance in leaves (K leaf ), in particular, is still fairly limited. We hypothesized that the AQPs of the vascular bundle sheath (BS) cells regulate K leaf . To examine this hypothesis, AQP genes were silenced using artificial microRNAs that were expressed constitutively or specifically targeted to the BS. MicroRNA sequences were designed to target all five AQP genes from the PLASMA MEMBRANE-INTRINSIC PROTEIN1 (PIP1) subfamily. Our results show that the constitutively silenced PIP1 (35S promoter) plants had decreased PIP1 transcript and protein levels and decreased mesophyll and BS osmotic water permeability (P f ), mesophyll conductance of CO 2 , photosynthesis, K leaf , transpiration, and shoot biomass. Plants in which the PIP1 subfamily was silenced only in the BS (SCARECROW:microRNA plants) exhibited decreased mesophyll and BS P f and decreased K leaf but no decreases in the rest of the parameters listed above, with the net result of increased shoot biomass. We excluded the possibility of SCARECROW promoter activity in the mesophyll. Hence, the fact that SCARECROW:microRNA mesophyll exhibited reduced P f , but not reduced mesophyll conductance of CO 2 , suggests that the BS-mesophyll hydraulic continuum acts as a feed-forward control signal. The role of AQPs in the hierarchy of the hydraulic signal pathway controlling leaf water status under normal and limited-water conditions is discussed.

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Role of aquaporins in determining transpiration and photosynthesis in water‐stressed plants: crop water‐use efficiency, growth and yield

Cited by 203 publications

References 106 publications

Response of Mid-Rotation Loblolly Pine (Pinus taeda L.) Physiology and Productivity to Sustained, Moderate Drought on the Western Edge of the Range

Response of Mid-Rotation Loblolly Pine (Pinus taeda L.) Physiology and Productivity to Sustained, Moderate Drought on the Western Edge of the Range

Outside-Xylem Vulnerability, Not Xylem Embolism, Controls Leaf Hydraulic Decline during Dehydration

The Role of Plasma Membrane Aquaporins in Regulating the Bundle Sheath-Mesophyll Continuum and Leaf Hydraulics

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