Role of arginine and fluoride in the prevention of eroded enamel: anin vitromodel

Abstract

Background: The aim of this study was to investigate the effect of arginine and fluoride on the reduction of erosive wear. Methods: Bovine enamel blocks were randomly allocated into four groups (n = 20) and exposed to: ESPR group (8% arginine, 1450 ppm sodium monofluorophosphate, calcium carbonate and titanium dioxide); ESen group (1450 ppm sodium monofluorophosphate, 5% potassium citrate); positive control PC group (1500 ppm sodium monofluorophosphate) and negative control NC group (water). The samples were submitted to six alternating cycles of demineralization-remineralization (cola, 10 minutes; artificial saliva, 1 hour, respectively). Before and between cyclic demineralization and remineralization, blocks were treated with slurries of the respective toothpastes or water (1 minute). Erosive tissue loss was analysed by microhardness and profilometry. Data were analysed by ANOVA and Tukey tests for individual comparisons among the groups (p < 0.05). Results: In microhardness, the ESPR (217.46 AE 55.45) group was significantly better than the other treatment groups (PC = 302.76 AE 96.10; ESen = 315.56 AE 74.56; p < 0.001). The ESPR group showed a similar loss to NC group (NC = 210.8 AE 49.98; p = 0.991). The mean erosion depth (+/-SE, lm) was detected between NC (14.37 AE 1.72) and dentifrices tested (ESPR (4.11 AE 1.34), ESen group (7.64 AE 1.61) and PC (8.20 AE 2.19) (p = 0.000). Conclusions: From the results of the present study, the effectiveness of Sensitive Pro Relief in the prevention of erosive surface loss seems to be attributed to the possible effect of the arginine associated with fluoride.

Nanoscratch testing for the assessment of enamel demineralization under conditions simulating wine erosion

“…erosion depth or volume), as well as changes in chemical composition, in accordance with the methodology that has been proposed for use in dental phenomics research . The use of new generation profilometers with nano‐scale resolution, state‐of‐the‐art wear‐testing devices with atomic scale resolution, and other established and emerging techniques also have the potential to broaden the scope of erosion research. An holistic view of wine erosion can be obtained by assessing its effect throughout the entire thickness of enamel and dentine, given that resistance of enamel to erosive wear has been found to decrease from the surface towards the dentino‐enamel junction .…”

Three-dimensional profilometric assessment of early enamel erosion simulating gastric regurgitation

“…33 The present model is a proof of concept for profilometric detection of early erosion and is an advance in profilometric assessment for several reasons: (1) A higher sensitivity of the profilometer in this study enabled earlier changes in surface roughness (i.e. after 30 s of erosion) to be detected compared with the longer durations described previously, [21][22][23][34][35][36] (2) Enamel specimens were polished flat to a level of 0.25 mm compared with approximately less than 9.0 mm in recent atomic force microscopic studies, or to 1.0 mm in surface nanohardness studies, 23,37,38 (3) The longitudinal design incorporated both quantitative and qualitative assessments (without the need for surface coating or negative impressions for qualitative assessment), (4) 3D surface roughness (Sa) was measured over areas of 43 mm  43 mm compared with linear measurements in previous studies, 34,39 and (5) The Sa values were collected longitudinally from the same areas of enamel. Our detection sensitivity for profilometric changes in the Sa values is comparable to those of between one and two minutes in recent reports, [39][40][41][42] and, collectively, these findings add to the need to diagnose the initial stages of early erosion as soon as possible in the clinic.…”

Çocuklarda Dental Erozyon Ve Koruyucu Uygulamalar

“…60 Başka bir in-vitro araştırmada, piyasada yer alan çeşitli diş macunlarının erozyon oluşumunu önlemedeki etkinliği değerlendirilmiş ve florürle birlikte arginin içeren diş macunlarının eroziv diş aşınmalarından korunmada daha etkili olduğu sonucuna varılmıştır. 61 Şekersiz sakız çiğnenerek tükürük akışının arttırılmasıyla da erozyon fenomeninde remineralizasyonun arttırılabileceği gösterilmiştir. 62 Minenin çözün-meye karşı direncini arttırmak amacıyla kullanılabilen bir diğer yöntem ise, kazein fosfopeptit-amorf kalsiyum fosfat (CPP-ACP) uygulamalarıdır.…”