Role of Ascorbic Acid in Periodontal Ligament Cell Differentiation

Ishikawa, Satoshi; Iwasaki, Kengo; Komaki, Motohiro; Ishikawa, Isao

doi:10.1902/jop.2004.75.5.709

Cited by 63 publications

(62 citation statements)

References 30 publications

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“…Local differentiation of PDL fibroblasts into osteoblasts and cementoblasts near the bone and cementum surfaces is most likely tightly controlled. Studies have shown that many different factors including both platelet-derived growth factor and transforming growth factor ␤ 1 [Han et al, 2007], BMP2 Yamada et al, 2007], ascorbic acid [Ishikawa et al, 2004;Mimori et al, 2007], and amelogenin and ameloblastin [Zeichner-David et al, 2006] stimulate osteogenic differentiation of PDL fibroblasts. Factors like the transcription factor Msx2 [Yoshizawa et al, 2004], Twist and the proteoglycan PLAP-1/asporin [Yamada et al, 2007] have been implicated in inhibiting such osteogenic differentiation.…”

Section: μMmentioning

confidence: 99%

Inorganic Phosphate Stimulates DMP1 Expression in Human Periodontal Ligament Fibroblasts Embedded in Three-Dimensional Collagen Gels

Berendsen

Smit

Schoenmaker

et al. 2010

Cells Tissues Organs

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Stable integration of collagenous tissue-engineered constructs to surrounding solid devices can be accomplished by coating the solid surfaces with exogenous alkaline phosphatase (ALP). We showed previously that coating of culture well surfaces with the enzyme in combination with the presence of its substrate β-glycerophosphate (β-GP) induces mineral deposition at the interface of matrix and surface, thereby preventing matrix detachment. In this study the effect of such mineral-inducing conditions on differentiation of human periodontal ligament (PDL) fibroblasts into osteoblasts/cementoblasts was analyzed in three-dimensional collagen gels. Mineral-inducing conditions decreased collagen type I gene expression and induced dentin matrix protein 1 (DMP1; a marker of late osteoblasts/cementoblasts) gene expression by fibroblasts. DMP1 protein was detected in some fibroblasts only in mineralizing gels. Exogenous ALP released high levels of inorganic phosphate from β-GP. Addition of inorganic phosphate alone induced DMP1 gene expression, which could be prevented by blocking phosphate entry into fibroblasts by foscarnet. We concluded that mineralizing conditions induced by exogenous ALP affect the phenotype of PDL fibroblasts. The fibroblasts are stimulated to express the late osteoblast/osteocyte marker protein DMP1, which is mediated by uptake of inorganic phosphate into the cells. The enzyme-mediated mineral deposition may thus facilitate enhanced integration of collagenous tissue-engineered constructs to devices or implants in vitro.

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Section: μMmentioning

confidence: 99%

Inorganic Phosphate Stimulates DMP1 Expression in Human Periodontal Ligament Fibroblasts Embedded in Three-Dimensional Collagen Gels

Berendsen

Smit

Schoenmaker

et al. 2010

Cells Tissues Organs

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“…Ishikawa et al studied the effect of ascorbic acid on PDL cells and observed that ascorbic acid increased the ALP activity, which is required for the binding of PDL cells to Type I collagen through 2 beta 1 integrin, whose expression is again increased by ascorbic acid. As Type I collagen production is considered an initial process in the differentiation of PDL cells, it may serve as a potential storage medium [18]. 0.14 g/L potassium phosphate; 0.14 g/L calcium chloride; 0.1 g/L magnesium chloride; and 0.1 g/L magnesium sulfate.…”

Section: Ascorbic Acidmentioning

confidence: 99%

Current Developments in Transport Media for Avulsed Teeth: An Update

Roma

Hegde

2017

Asian J Pharm Clin Res

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Dental avulsion is one of the common types of trauma that results in the complete displacement of tooth from the alveolar socket. Although the ideal treatment would be the immediate reimplantation of the tooth at the site where the trauma took place, this may not be practically possible in every case. Hence, the avulsed tooth may have to be placed in an appropriate storage or transport medium until it is reimplanted. The biological properties of the storage medium have a significant impact on the success of reimplantation, as it must be capable of preserving the vitality, clonogenic, and mitogenic ability of the periodontal ligament cells for successful reimplantation.

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“…It should be noted that this may have consequences for the function of prolylhydroxylase which cofactor is ascorbic acid. Moreover, vitamin C deficiency may influence the collagen structure and extracellular matrix via promotion of the osteoblasts differentiation by modulating type I collagen-α2β1 integrin interactions [40], triggering the aggregated collagensynthesizing cells to organize their extracellular matrix [41], regulating proteoglycans biosynthesis and turnover [42] and altering mRNA levels in collagensynthesizing cells by changing the transcription rates [43]. Additionally, ascorbic acid also induced alkaline phosphatase activity, the expression of mRNAs for proteins that are markers of osteoblastic differentiation, the deposition of calcium [44] and increase of the rate of procollagen secretion from cells into extracellular matrix [45].…”

Section: T a B L E 1 Initial And Final Body Weights And Blood Glucosmentioning

confidence: 99%

Effect of nicotinamide on amino acids content in bone collagen depending on biological availability of vitamins in diabetic rats

Guzyk¹,

Sergiichuk²,

Dyakun³

et al. 2014

Ukr.Biochem.J.

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connective tissue is highly susceptible to imbalances induced by diabetes. Diabetes-related osteopenia, decreased bone strength etc. may be associated with altered metabolism of various collagens. although it is assumed that alterations in collagen amino acids (aa) may strongly affect protein properties and physiological functions, however, very limited evidences are present at the moment regarding aa composition of bone type I collagen and its relevance to abnormal availability of vitamins which are necessary for collagen synthesis in diabetes. We have tested whether nicotinamide (NAm) can influence type I collagen formation and aa composition as well as vitamins availability in diabetes. after 4 weeks of STz-induced diabetes (60 mg/ kg) male Wistar rats were injected for 2 weeks with/without Nam (200 mg/kg b. w.). acid extraction of type I collagen from the bones was performed with following stepwise salting out. The content of type I collagen after its acid extraction from the bones was estimated by the amounts of hydroxyproline. amino acids were assayed by cation exchange chromatography. Diabetes-associated changes in aa composition of type I collagen mainly affect those amino acids which are known to be involved in helix formation and cross-linking of the molecules. Diabetes was found to significantly reduce bone collagen contents of o- Pro, Gly, Ala, whereas Lys, his, arg, Glu, Thr, Leu, Phe contents were elevated (P < 0.05 bone collagen amino acids, vitamin c, vitamin B 3 , T ype 1 diabetes results from active immunemediated destruction of insulin-producing β-cells in the pancreas by T-cells and macrophages infiltrating the islets [1]. It is likely that many pathways may lead to autoimmune destruction of the islet β-cells and actual mechanism in each individual depends on genetics and environment [2].The results of numerous investigations clearly demonstrate that modern diabetes pharmacological treatment is unable to prevent completely diabetes complications among which cardiovascular disease and nephropathy have the highest levels of incidence and the poorest prognosis [3,4]. It is known that during this disease proteins are targets for numerous injuries and could have pathologically altered turnover rates.Connective tissue disturbances and especially qualitative changes of collagen synthesis always accompany these pathological processes [5]. Diabetes induces structural and functional changes of the extracellular matrix proteins in many tissues [6]. It could not be excluded that alterations in levels of bone collagen amino acids may contribute to diabetes.Processing and secretion of collagen was previously shown to be controlled by insulin at a post- [7]. Other authors demonstrated that the α2(I) collagen gene contains two functional promoters, and its expression in cells is regulated at both transcriptional and post-transcriptional levels [8]. On the other hand there were no evidences of qualitative changes in connective tissue collagens caused by lack of insulin. Diabetes-related connective tissue ch...

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Role of Ascorbic Acid in Periodontal Ligament Cell Differentiation

Cited by 63 publications

References 30 publications

Inorganic Phosphate Stimulates DMP1 Expression in Human Periodontal Ligament Fibroblasts Embedded in Three-Dimensional Collagen Gels

Inorganic Phosphate Stimulates DMP1 Expression in Human Periodontal Ligament Fibroblasts Embedded in Three-Dimensional Collagen Gels

Current Developments in Transport Media for Avulsed Teeth: An Update

Effect of nicotinamide on amino acids content in bone collagen depending on biological availability of vitamins in diabetic rats

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