Role of ATP and sodium in polyamine transport in bovine pulmonary artery smooth cells

Aziz, Shewan M.; Lipke, David W.; Olson, Jack W.; Gillespie, Mark N.

doi:10.1016/0006-2952(94)90206-2

Cited by 15 publications

(12 citation statements)

References 16 publications

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“…However, the bioenergetics of polyamine transport and the ionic gradients to which it may be coupled remain unclear. Moreover, although the existence of polyamine transport activities in both prokaryotic and some eukaryotic cells has been thoroughly established [15][16][17][19][20][21][22][23][24], only the prokaryote and unicellular eukaryote transporters have been identified [25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Transport of polyamines in Drosophila S2 cells: kinetics, pharmacology and dependence on the plasma membrane proton gradient

Romero-Calderón

Krantz

2005

Biochemical Journal

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Polyamine transport activities have been described in diverse multicellular systems, but their bioenergetic mechanisms and molecular identity remain unclear. In the present paper, we describe a high-affinity spermine/spermidine transport activity expressed in Drosophila S2 cells. Ion-replacement experiments indicate that polyamine uptake across the cell membrane is Na+-, K+-, Cl-- and Ca2+-independent, but pH-sensitive. Additional experiments using ionophores suggest that polyamine uptake may be H+-coupled. Pharmacological experiments show that polyamine uptake in S2 cells is selectively blocked by MGBG {methylglyoxal bis(guanylhydrazone) or 1,1'-[(methylethanediylidine)-dinitrilo]diguanidine} and paraquat (N,N-dimethyl-4,4'-bipyridylium), two known inhibitors of polyamine uptake in mammalian cells. In addition, inhibitors known to block the Slc22 (solute carrier 22) family of organic anion/cation transporters inhibit spermine uptake in S2 cells. These data and the genetic tools available in Drosophila will facilitate the molecular identification and further characterization of this activity.

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Section: Introductionmentioning

confidence: 99%

Transport of polyamines in Drosophila S2 cells: kinetics, pharmacology and dependence on the plasma membrane proton gradient

Romero-Calderón

Krantz

2005

Biochemical Journal

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“…In several studies putrescine uptake seemed to function as an Na + -dependent cotransport system based on the decrease of uptake when extracellular NaCl was isoosmotically replaced by other electrolytes such as choline chloride (Feige and Chambaz 1985;Parys et al 1990;Alves et al 1992;Aziz et al 1994). However, in the meantime evidence has been presented that the inhibition of putrescine uptake by Na + indicates that changes in the membrane potential could modulate polyamine uptake (Kumagai and Johnson 1988;Dot et al 2000).…”

Section: Discussionmentioning

confidence: 93%

“…In previous studies it has been shown that the pharmacological properties of the putrescine transporter considerably vary between the species investigated (Aziz et al 1994;Marton and Pegg 1995;Igarashi and Kashiwagi 1999). Hence, to prove or reject the identity of the agmatine accumulation mechanism with the putrescine transporter, the pharmacological profiles of both systems should be compared within the same cell type or at least within the same species.…”

Section: Discussionmentioning

confidence: 98%

Agmatine and putrescine uptake in the human glioma cell line SK-MG-1

Molderings

Bönisch

Göthert

et al. 2001

Naunyn-Schmiedeberg's Archives of Pharmacology

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The pharmacological properties of a specific agmatine uptake mechanism were investigated in the human glioma cell line SK-MG-1 and compared with those of the putrescine transporter expressed by the same cells and with those of several other organic cation transport systems or ion channels reported in the literature. The specific accumulation of [14C]agmatine at 37 degrees C above nonspecific accumulation at 4 degrees C was energy-dependent and saturable with a Vmax of 64.3+/-3.5 nmol/min per mg protein and a Km of 8.6+/-1.4 microM. Specific accumulation was attenuated by replacement of extracellular Na+ by choline by 65%, not affected by lithium and enhanced by replacement by sucrose. Phentolamine, clonidine, 1,3-di(2-tolyl)guanidine, histamine, putrescine, spermine and spermidine were inhibitors of specific [14C]agmatine accumulation. In contrast, corticosterone, desipramine, O-methylisoprenaline, cirazoline, moxonidine, L-arginine, L-lysine, verapamil, nifedipine and CdCl2 at concentrations up to 10 mM failed to inhibit specific [14C]agmatine accumulation, thus excluding that the latter is mediated by amino acid or monoamine carriers, by Ca2+ channels or by the organic cation transporters OCT1, OCT2, OCT3, OCTN1 or OCTN2. The pattern of activity of inhibitory compounds was also different from that determined for specific putrescine accumulation found in the same cells (Km 1.3+/-0.1 microM, Vmax 26.1+/-0.4 nmol/min per mg protein) ruling out an identity of the specific [14C]agmatine and [14C]putrescine accumulation mechanisms. It is concluded that specific accumulation of agmatine in human glioma cells is mediated by a specific transporter whose pharmacological properties are not identical to those of the agmatine transporter previously identified in rat brain synaptosomes and to other so far known carrier mechanisms for organic cations and ion channels. The agmatine uptake system may be important for the regulation of the extracellular concentration of agmatine in man.

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“…Considering the reported mechanism of accumulation of paraquat in the lung tissue, and some reports that points to a Na þ dependence of the polyamine uptake system (Janne et al 1978;Rinehart and Chen 1984;Kumagai and Johnson 1988;Rannels et al 1989;Aziz et al 1994), it might be suggested that paraquat uptake into the extravascular compartments of the lung (alveolar interstitium and epithelium) is prevented by Na þ depletion. In contrast, some reports evidence no Na þ requirement for putrescine uptake by different cell types (Lewis et al 1989;Morgan 1992).…”

Section: Discussionmentioning

confidence: 95%

“…Na þ replacement by Li þ was also demonstrated to inhibit significantly putrescine uptake by rat isolated enterocytes (Kumagai and Johnson 1988). In bovine arterial smooth muscle cells, Janne et al (1978) and Aziz et al (1994) demonstrated some Na þ dependence for polyamine uptake. Rannels et al (1989) found that in type II pneumocytes the uptake of putrescine and spermidine was dependent on Na þ , whereas spermine uptake was not dependent on extracellular Na þ , indicating that polyamine uptake may take place via different transporters systems.…”

Section: Introductionmentioning

confidence: 90%

Kinetics of paraquat in the isolated rat lung: Influence of sodium depletion

Dinis-Oliveira

Valle²,

Bastos³

et al. 2006

Xenobiotica

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Paraquat accumulates in the lung through a characteristic polyamine uptake system. It has been previously shown that paraquat uptake can be significantly prevented if extracellular sodium (Na+) is reduced, although the available data correspond to experiments performed using tissue slices or incubated cells. This type of in vitro study fails to give information on the actual behaviour occurring in vivo since the anatomy and physiology of the studied tissue is disrupted. Accordingly, the aim of the present study was to explore the usefulness of the isolated rat lung model when applied to characterize the kinetic behaviour of paraquat in this tissue after bolus injection under standard experimental conditions as well as to evaluate the influence of iso-osmotic replacement of Na+ by lithium (Li+) in the perfusion medium. The obtained results show that the present isolated rat lung model is useful for the analysis of paraquat toxicokinetics, which is reported herein for the first time. It was also observed that Na+ depletion in the perfusion medium leads to a decreased uptake of paraquat in the isolated rat lung, although it seems that this condition does not contribute to improve the elimination of paraquat once the herbicide reaches the extravascular structures of the tissue, since the paraquat tissue wash-out phase is similar under both experimental conditions assayed.

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Role of ATP and sodium in polyamine transport in bovine pulmonary artery smooth cells

Cited by 15 publications

References 16 publications

Transport of polyamines in Drosophila S2 cells: kinetics, pharmacology and dependence on the plasma membrane proton gradient

Transport of polyamines in Drosophila S2 cells: kinetics, pharmacology and dependence on the plasma membrane proton gradient

Agmatine and putrescine uptake in the human glioma cell line SK-MG-1

Kinetics of paraquat in the isolated rat lung: Influence of sodium depletion

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