Role of Atrial Natriuretic Factor in Regulation of Blood Pressure in Normotensive Rats Having Reduced Renal Mass

Pamnani, Motilal B.; Mueller, Gregory P.; Ghai, R. D.; Haddy, Francis J.

doi:10.3181/00379727-189-42810

Cited by 14 publications

(12 citation statements)

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“…ANP infusion consistently, but again transiently, decreases blood pressure in certain types of hypertension (8). However, when we immunized normotensive rats (60% reduced renal mass) with X-80 antisera against ANP, these animals developed sustained elevation of blood pressure, suggesting a role for endogenous ANP in the regulation of blood pressure on a chronic basis (9). Similar results were reported by Lohmeier et al (10) regarding the role of ANP in longterm volume homeostasis.…”

Section: Introductionsupporting

confidence: 55%

“…The antihypertensive effect is independent of the mechanism of hypertension and results from an effect on peripheral vasculature. We have previously shown that in vivo blockade of ANP with ANP antibodies causes an increase in blood pressure (9). Taken together these studies suggest that under certain conditions ANP plays a role in blood pressure regulation.…”

Section: Discussionmentioning

confidence: 81%

“…The residue was dissolved in RIA buffer for radioimmunoassay according to the manufacturer's direction. Preliminary tests indicated that the antibodies used in the assay did not cross react with C-ANF (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23) at concentrations as high as 100 M. The lowest concentration of r-ANP detected was 5 pg/ml.…”

Section: Measurement Of Plasma Anp Levelsmentioning

confidence: 97%

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Effects of a Neutral Endoprotease Enzyme Inhibitor, Thiorphan, on Hemodynamics and Renal Excretory Function in Four Models of Experimental Hypertension

Pamnani

Chen²,

Bryant³

et al. 2000

Clinical and Experimental Hypertension

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Thiorphan, a neutral endoprotease (NEP) enzyme inhibitor, has been shown to enhance the effects of atrial natriuretic peptide (ANP) in vivo. In this study, we examined the effects of an intravenous (iv) infusion of thiorphan on cardiovascular hemodynamics and excretion of urine volume (UV), sodium (U(Na)V) and potassium (UKV) in four different models of experimental hypertension, namely: 1) SHR, 2) two-kidney, one clip (2K1C),3) one-kidney, 1 clip (1K1C) and. 4) 70% reduced renal mass-salt (RRM-S) hypertensive rats. SHR has normal plasma renin activity, 2K1C is renin dependent, and 1K1C and RRM-S are low renin volume dependent models of hypertension. Rats were divided into experimental and control groups. Under inactin (120 mg/kg, body weight) anesthesia, rats were instrumented to record blood pressure and dP/dt (Millar catheter) and urine was collected through a suprapubic urinary bladder catheter. Experimental animals received an iv infusion of thiorphan, 0.5 mg/kg/min for 120 minutes. Control animals received vehicle only. In some animals, vascular smooth muscle cell membrane potentials (Em) was measured in vivo. In another series of experiments, using the identical protocol, cardiac output was recorded. The thiorphan infusion produced a similar progressive decrease in blood pressure in all models of hypertension. Cardiac output did not change relative to vehicle infused control animals. Thus pressure decreased because of a decrease in total peripheral resistance. The contractility index (dP/dt/P, where P = left ventricular pressure) did not change but vascular smooth muscle cells in tail arteries hyperpolarized in all four models. In spite of a significant decrease in blood pressure, thiorphan infusion either increased or produced no change in urinary volume (UV) and sodium (U(Na)V) excretion. These data show that thiorphan, an NEP inhibitor, decreases the blood pressure of hypertensive rats due to a decrease in total peripheral resistance, perhaps by hyperpolarizing vascular smooth muscle cells. These effects are independent of the mechanism of the hypertension. Increased UV and U(Na)V in the face of decreased pressure suggests a direct renal effect.

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Section: Introductionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 81%

Section: Measurement Of Plasma Anp Levelsmentioning

confidence: 97%

See 1 more Smart Citation

Effects of a Neutral Endoprotease Enzyme Inhibitor, Thiorphan, on Hemodynamics and Renal Excretory Function in Four Models of Experimental Hypertension

Pamnani

Chen²,

Bryant³

et al. 2000

Clinical and Experimental Hypertension

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“…It seems unlikely that a genetic nephron deficiency of even 25% in some hypertensive rat models could explain development of hypertension without other morphological or functional renal abnormalities. Surgical reduction of renal mass must be at least 60-70% before hypertension develops in rats drinking physiological saline [25].…”

Section: Discussionmentioning

confidence: 99%

Renal functional, not morphological, abnormalities account for salt sensitivity in Dahl rats

Manger

Simchon

Stokes

et al. 2009

Journal of Hypertension

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“…Previous reports indicated that the development of hypertension depends on a reduction in renal mass. For example, when the renal mass was decreased by more than 60 % in Wistar rats, either expansion of the extracellular fluid [12,22] or administration of a Na + -K + inhibitor [6,20,21] could induce hypertension without coexisting diabetes. However, when the renal mass was decreased by less than 60 %, hypertension did not develop under the same conditions.…”

Section: Changes In Blood Pressurementioning

confidence: 98%

Effects of streptozotocin and unilateral nephrectomy on L-type Ca2+ channels and membrane capacitance in arteriolar smooth muscle cells

Youm

Park

Jang

et al. 2014

Pflugers Arch - Eur J Physiol

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Diabetes mellitus and hypertension are common diseases frequently coexisting. Although augmentation of L-type Ca(2+) channel (ICaL) activity has been reported in vascular smooth muscle cells (VSMCs) of a spontaneously hypertensive rat model, no study on ICaL has been conducted for coexisting hypertension and diabetes. Sprague Dawley rats were assigned to four groups: a sham-operated control group (CG), a unilateral nephrectomy group (UNG), a streptozotocin (STZ)-induced type 1 diabetic group (SDG) and a coexisting hypertension and diabetes group (DHG), which underwent nephrectomy and received STZ injection. Blood pressure (BP) was significantly lower in the CG than in the other three groups. The membrane capacitance of VSMCs was nearly doubled in the SDG and DHG but not in the UNG. The ICaL was increased approximately 2-fold in both the UNG and SDG and approximately 4-fold in the DHG. The current density of ICaL was increased approximately 2-fold in the UNG and DHG, while no significant increase was seen in the SDG. The rate of Ca(2+) removal was inhibited significantly, by ~33 %, in the DHG. In conclusion, the effects of hypertension and diabetes on ICaL were apparently additive, and the vascular consequences of combined diabetes and hypertension may be caused by an elevated ICaL and slowed Ca(2+) removal.

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Role of Atrial Natriuretic Factor in Regulation of Blood Pressure in Normotensive Rats Having Reduced Renal Mass

Cited by 14 publications

References 0 publications

Effects of a Neutral Endoprotease Enzyme Inhibitor, Thiorphan, on Hemodynamics and Renal Excretory Function in Four Models of Experimental Hypertension

Effects of a Neutral Endoprotease Enzyme Inhibitor, Thiorphan, on Hemodynamics and Renal Excretory Function in Four Models of Experimental Hypertension

Renal functional, not morphological, abnormalities account for salt sensitivity in Dahl rats

Effects of streptozotocin and unilateral nephrectomy on L-type Ca2+ channels and membrane capacitance in arteriolar smooth muscle cells

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