Role of auxins, polyamines and ethylene in root formation and growth in sweet orange

Mendes, Amanda F. S.; Cidade, Luciana C.; Otoni, Wagner Campos; Soares-Filho, Walter S.; Costa, Márcio Gilberto Cardoso

doi:10.1007/s10535-011-0058-y

Cited by 44 publications

(32 citation statements)

References 22 publications

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“…In the present study, NAA was also proved to be efficient by morphological and anatomical observation of the rooting process (Figure 1), with the same observable results as in apple microcuttings [32], Petunia hybrida [33] and Fuchsia hybrida cuttings [34]. On the other hand, the timing of root initiation may be critical in determining the optimal application time for hormones [35,36]. The AR primordia and root emergence were evident by 12 days in those cuttings treated with NAA ( Figure 1) in our experiment, similar to the time of root emergence in cuttings of Eucalyptus globulus × E. maidenii [37] and of eucalypt hybrids [38].…”

Section: Discussionsupporting

confidence: 75%

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Shao

Yang

Fang

et al. 2017

Forests

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In the present study, an attempt was made to induce rooting from single-node cuttings of hybrid aspen (Populus tremula L. × P. tremuloides Michx.) with different concentrations of Indole-3-acetic acid (IAA), Indole-3-Butytric acid (IBA) and 1-Naphthylacetic acid (NAA). Among the three auxins used, 0.54 mM NAA showed more effective induction on rooting as compared to IAA and IBA at the whole level. Thereafter, 0.54 mM NAA was used further for the anatomical and biochemical investigation. The results showed that it took 12 days from the differentiation of primordium to the appearance of young adventitious roots under NAA application. It was found that endogenous IAA, Zeatin riboside (ZR) and Gibberellic Acid (GA3) levels increased, but Abscisic acid (ABA) decreased in cuttings with NAA treatment. In contrast to the endogenous IAA level, NAA resulted in a decrease in IAA-oxidase (IAAO) activity. Similarly, the decreased peroxidase (POD) activity, consistent with down-regulation of expressed levels of POD1 and POD2, was observed in NAA-treated cuttings. Moreover, NAA resulted in a higher activity in polyphenol oxidase (PPO) compared with control cuttings. Collectively, the study highlighted that 0.54 mM NAA is efficient on rooting in hybrid aspen, and its effect on metabolic changes during rooting was discussed, which can provide valuable information for propagating hybrid aspen.

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Section: Discussionsupporting

confidence: 75%

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Shao

Yang

Fang

et al. 2017

Forests

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“…On the other hand, the timing of root initiation may be critical in determining the optimal application time for hormones [33,34].The adventitious root primordia and root emergence were evident by 12 days in those treated cuttings with NAA ( Figure 1) in our experiment, similar to the time of root emergence in cuttings of Eucalyptus globulus x E. maidenii [38] and of eucalypt hybrids [39].…”

Section: Discussionsupporting

confidence: 73%

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Shao¹,

Yang²,

Fang³

et al. 2017

Preprint

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In the present study, an attempt was made to induce rooting from single-node cuttings of hybrid aspen (Populus tremula L. ×P. tremuloides Michx.) with different concentrations of IAA, IBA and NAA during rooting. Among the three auxins used, NAA showed more effective induction on rooting as compared to IAA and IBA at the whole level. Thereafter, NAA was used further in experiments for anatomical and biochemical investigation. The results showed that it took 12 days from the differentiation of primordium to the appearance of young adventitious roots with NAA application. It was found that endogenous IAA, ZR and GA3 levels increased, but ABA decreased in cuttings with 0.54 mM NAA treatment. In contrast to the endogenous IAA level, NAA had negative effect on IAA-oxidase (IAAO) activity. Similarly, the decreased peroxidase (POD) activity, consistent with down-regulation of expressed levels of POD1 and POD2, was observed in NAAtreated cuttings. Whereas, NAA resulted in a higher activity in polyphenol oxidase (PPO) compared to the control cuttings. Collectively, the study highlighted that 0.54 mM NAA is efficient on rooting in hybrid aspen, and its effect on metabolic changes during rooting is discussed, which provide valuable information for propagating hybrid aspen.

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“…However, some researchers have found that ethylene is a key stimulatory factor in the formation of root primordial (Ivanchenko et al 2008;Liu et al 1990). The ethylene synthesis inhibitor AgNO 3 inhibited the adventitious root formation of in vitro rooting in sweet orange (Mendes et al 2011) and chrysanthemum cuttings ).…”

Section: Functional Analysis Of the Identified Proteinsmentioning

confidence: 99%

“…In addition, decarboxylated SAM acts in the biosynthesis of polyamines and as a precursor for ethylene biosynthesis in higher plants (Gomez-Gomez and Carrasco 1996). Polyamines and the ethylene biosynthetic pathways are competitive (Argueso et al 2007), and several studies have shown that polyamines play a key role in rooting (Biondi et al 1990;Friedman et al 1982Friedman et al , 1985Jarvis and Yasmin 1987;Mendes et al 2011). Furthermore, polyamines play a regulatory role during active cell division and the initiation of root primordia (Nag et al 2001).…”

Section: Functional Analysis Of the Identified Proteinsmentioning

confidence: 99%

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Sheng

Zhao

Zhang

et al. 2014

Trees

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Key message Comparative proteome analysis revealed the proteins that are differentially expressed during the processes of tetraploid black locust (Robinia pseudoacacia L.) adventitious root formation, which are involved in carbohydrate binding, energy and metabolism, and stress response. Abstract The tetraploid black locust (Robinia pseudoacacia L.) is an important fast-growing tree used in afforestation and is also a protein-rich feed for poultry. Currently, the primary approach for tetraploid black locust reproduction is through cutting propagation. To identify the specific proteins in the rooting process, including the formation of adventitious root primordium (ARP) and the elongation of adventitious root (EAR), a comparative proteomic analysis of the ARP and EAR of tetraploid black locust was performed to identify related proteins that may be involved in regulating ARP formation and the process of EAR. High-resolution two-dimensional electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric (MS) analysis was used to identify differentially expressed proteins in ARP and EAR. A total of 84 protein spots showed significant expression changes by 2-DE and were successfully identified by MALDI TOF/ TOF MS/MS. Of these, 29 proteins were differentially expressed during the ARP phase, and the remaining 55 proteins did so in the EAR phase. The majority of identified proteins were classified into functional categories, including carbohydrate binding, energy and metabolism, protein degradation/folding and import, cellular cytoskeleton, hormonerelated and oxidation reactions, stress response, cell proliferation, and transcription regulation. Quantitative real-time PCR analysis was performed for selected proteins and showed that not all of the protein expression levels were consistent with the mRNA levels. This study provides the basis for further functional studies of differentially expressed proteins, which will contribute to the understanding of the biochemical processes in adventitious root primordium formation and the elongation of adventitious root. Abbreviations 2-DE Two-dimensional gel electrophoresis MALDI Matrix assisted laser desorption ionization TOF Time of flight TOF MS Time of flight mass spectrometry MS Mass spectrometry IntroductionTetraploid black locust (Robinia pseudoacacia L.) (TRP), which can be produced by doubling the chromosomes of a diploid black locust (2n 9 2) artificially, is a leguminous Communicated by R. Hampp. Electronic supplementary materialThe online version of this article (

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Role of auxins, polyamines and ethylene in root formation and growth in sweet orange

Cited by 44 publications

References 22 publications

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

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