Role of Bacteria as Biocolloids in the Transport of Actinides from a Deep Underground Radioactive Waste Repository

Francis, A.J.; Gillow, J. B.; Dodge, Cleveland J.; Dunn, Matthew; Mantione, K.; Strietelmeier, Betty A.; Pansoy-Hjelvik, M. E.; Papenguth, Hans W.

doi:10.1524/ract.1998.82.special-issue.347

Cited by 45 publications

(35 citation statements)

References 5 publications

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“…The growth of halophilic bacteria Halomonas sp. and mixed bacterial cultures consisting of Haloarcula sinaiiensis, Alteromonas sp, Marinobacter sp, and an unclassified species of yproteobacterium was retarded by 239P~-EDTA at concentrations > l. OxlO"M (Pansoy-Hjelvik, et al, 1997;Francis et al, 1998) (Figs. 2 and 3).…”

Section: Effect Of Puion Microorganismsmentioning

confidence: 99%

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Microbial transformations of plutonium and implications for its mobility

Francis

2001

Radioactivity in the Environment

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Section: Effect Of Puion Microorganismsmentioning

confidence: 99%

“…Uninoculated medium containing Pu served as the control (redrawn from Francis et al, 1998;Pansoy-Hjelvik et al, 1997). caused a significant reduction in the growth of fungi measured as CFU(colony forming units) compared to 239Pu(N03)4 and 239P~DTPA.…”

Section: Applmentioning

confidence: 99%

Microbial transformations of plutonium and implications for its mobility

Francis

2001

Radioactivity in the Environment

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“…Bacteria have a physiologically stress response on oxidative stress, induced by metal ions (Workentine et al 2008). They have a great potential to bind actinides and, thus, transport actinides in the environment (Francis et al 1998;Pedersen 2005;Lütke et al 2012). Several interaction mechanisms between microorganisms and heavy metals are known, some of which have the potential of a substantial retention of these metal ions.…”

Section: Introductionmentioning

confidence: 99%

A spectroscopic study on U(VI) biomineralization in cultivated Pseudomonas fluorescens biofilms isolated from granitic aquifers

Krawczyk-Bärsch

Lütke

Moll

et al. 2014

Environ Sci Pollut Res

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The interaction between the Pseudomonas fluorescens biofilm and U(VI) were studied using extended X-ray absorption fine structure spectroscopy (EXAFS), and time-resolved laser fluorescence spectroscopy (TRLFS). In EXAFS studies, the formation of a stable uranyl phosphate mineral, similar to autunite (Ca[UO2]2[PO4]2•2-6H2O) or meta-autunite (Ca[UO2]2[PO4]2•10-12H2O) was observed. This is the first time such a biomineralization process has been observed in P. fluorescens. Biomineralization occurs due to phosphate release from the cellular polyphosphate, likely as a cell's response to the added uranium. It differs significantly from the biosorption process occurring in the planktonic cells of the same strain. TRLFS studies of the uranium-contaminated nutrient medium identified aqueous Ca2UO2(CO3)3 and UO2(CO3)3 (4-) species, which in contrast to the biomineralization in the P. fluorescens biofilm, may contribute to the transport and migration of U(VI). The obtained results reveal that biofilms of P. fluorescens may play an important role in predicting the transport behavior of uranium in the environment. They will also contribute to the improvement of remediation methods in uranium-contaminated sites.

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“…To date, Gorman-Lewis et al (2005a) performed the only study evaluating the effect of ionic strength on the adsorption of neptunium (V) to a bacterial surface; showing a decrease in neptunium (V) adsorption to the non-halophilic Gram-positive bacterium Bacillus subtilis with increasing ionic strength over the range of 0.001 to 0.5 M. However, evaluation of the potential effects of ionic strength conditions greater than 0.5 M is critical for understanding the fate and transport characteristics of neptunium in high ionic strength aqueous natural environments, such as in the vicinity of salt-based nuclear waste repositories and high ionic-strength groundwater at DOE sites. To date, very few studies (Francis et al, 1998(Francis et al, , 2004Gillow et al, 2000) have evaluated the heavy metal adsorption potential of halotolerant and halophilic microorganisms, which dominate the microbial ecology in natural high ionic strength aqueous environments. Francis et al (1998) showed that halotolerant and halophilic bacteria and archaea can adsorb actinides, including neptunium, to an appreciable extent.…”

Section: Introductionmentioning

confidence: 99%