Role of basal shoot darkening and exogenous putrescine treatments on in vitro rooting and on endogenous polyamine changes in difficult-to-root woody species

Rugini, Eddo; Jacoboni, A.; Luppino, Maria

doi:10.1016/0304-4238(93)90138-g

Cited by 55 publications

(37 citation statements)

References 16 publications

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“…For this purpose, we looked at changes in polyamine levels in hormone-treated cuttings in the presence and absence of leaves. Polyamines have been implicated in vegetative rooting of stem cuttings in different species [22,23,[26][27][28]. Thus, consistent with previous reports, putrescine levels significantly increased in the bitter melon cuttings that were about to root; i.e., cuttings with one intact leaf and treated with hormone [23,27,28].…”

Section: Discussionsupporting

confidence: 89%

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The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Malik¹,

Pérez²,

Kunta³

2012

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The study was conducted to investigate the optimal hormone treatment for rooting in bitter melon and the effect of defoliation on rooting and polyamine levels. Commercial preparation (diluted 1:10 and 1:20) gave extensive rooting within five days after treatment. The presence of leaf with the stem cutting was necessary for optimal rooting as defoliation drastically reduced rooting even when the rooting time was extended from 5 to 10 days. The presence of leaf also promoted the levels of putrescine three days after the hormone treatment just at the time of root emergence. Cuttings that did not have leaf did not show an increase in putrescine levels and did not produce roots. Spermidine levels also increased in the cuttings that rooted but there was no change in the levels of spermine in the cuttings that rooted. It appears that putrescine and spermidine play a role in regulating rooting in bitter melon cuttings. The vegetative propagation conditions described show that it is a practically viable technique for small growers to produce large number of plantlets at the farm for selection and cultivation.

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Section: Discussionsupporting

confidence: 89%

“…Therefore, experiments were conducted to evaluate the role of leaf on rooting. In addition, changes in polyamine levels in the rooting zone of the cutting were also measured because polyamine have previously been implicated in the rooting phenomenon [22,23]. The results of these studies are described here.…”

Section: Introductionmentioning

confidence: 99%

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Malik¹,

Pérez²,

Kunta³

2012

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“…When the polyamines were combined with auxins, it promoted early rooting and increased the final rooting percentage and the number of roots per explant [21,22]. Putrescine is involved in root induction by increasing the activity of total peroxidases at the base of explants and promotes rapid root growth and increases rooting frequency [23].…”

Section: Rootingmentioning

confidence: 99%

Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations

2007

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-Multiple shoots of high quality were produced in vitro from nodal explants of Tectona grandis. An average of about 4 shoots/uninodal explant was obtained within 4 weeks of culture on Murashige and Skoog's (mMS) medium modified by 50% reduction in NH 4 NO 3 concentration, supplemented with benzylaminopurine (1.5 mg L −1 ); indole-3-butyric acid (0.01 mg L −1 ) and gibberellic acid (0.1 mg L −1 ). The latter was applied both in the medium and by soaking the nodal segments for 10 s. in a gibberellic acid solution of 100 mg L −1 . Hundred percent of shoots rooted cultured on modified MS medium containing IBA (0.5 mg L −1 ) and putrescine (160 mg L −1 ). Putrescine promoted both strong and highly ramified roots and fast growing shoots during the rooting phase, conditioning the plantlets for a good survival and quality. Plantlets were transferred to jiffy pots for a short acclimatization stage in greenhouse where they survived at 100%. This highly reproducible procedure can be adopted for large scale teak propagation. . La présence de la putrescine a permis d'obtenir des racines fortes et ramifiées, des pousses à croissance rapide pendant l'enracinement, donnant des plantes de qualité aptes à une bonne survie. Les plantes ont été endurcies et transférées dans des Jiffy pots pour une acclimatation brève en conditions de serre ayant pour résultat 100% de survie. Ce procédé hautement reproductible peut être adapté à grande échelle dans la propagation de teak du fait de l'efficacité du nombre de pousses obtenues (prolifération), de l'enracinement, de l'acclimatation et du bon développement des plantes. régulateurs de croissance / putrescine / enracinement / explants nodaux / acide gibbérélique

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“…Significant increases on the rooting rates were achieved by Rugini et al (1993) by darkening the basal explants after painting in black the outside of the vessels and by placing black sterile polycarbonate granules on the surface of the solidified medium. Looking into replacing Rugini's interesting but cumbersome technique with a simpler and more economic alternative method, Mencuccini (2003) reported rooting rates between 86 and 100% on its trials with three Italian olive cultivars using the Brilliant Black commercial dye from Sigma 1 for medium darkening.…”

Section: Introductionmentioning

confidence: 99%

Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation

Peixe

Raposo

Lourenço

et al. 2007

Scientia Horticulturae

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The data presented report on trials conducted during 24 months using the Portuguese olive cultivar 'Galega vulgar'. The effectiveness of coconut water, BAP, or kinetin, as possible zeatin substitutes in olive micropropagation protocols, was investigated. In all stages of the micropropagation process, the mineral and vitamin formulation of olive medium (OM) was used. Regarding culture establishment the best results were achieved when 50 ml l À1 coconut water and 2.22 mM BAP were used as medium supplements. For the in vitro multiplication stage, the highest proliferation rates with an average of 3.4 new explants on each 30 days were achieved maintaining the coconut water concentration at 50 ml l À1 and increasing BAP up to 8.87 mM. The effects of IBA and activated charcoal on the in vitro root induction were also studied. Rooting rates of over 85% were obtained by basal immersion of the explants in IBA solution at 3 g l À1 for 10 s, followed by inoculation in the OM culture medium, added with 2 g l À1 of activated charcoal and without growth regulators. All in vitro rooted plants were transferred into Jiffy-Pots filled with vermiculite-perlite 3:1 (v/v) substrate. Those were subsequently wetted with the OM mineral solution, placed into polystyrene plates each one with 100 Jiffy-Pots capacity, which were transferred to traditional rooting mist benches, on a water-cooling equipped greenhouse. Such a simple acclimatization procedure allowed for 95% of plants survival. #

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Role of basal shoot darkening and exogenous putrescine treatments on in vitro rooting and on endogenous polyamine changes in difficult-to-root woody species

Cited by 55 publications

References 16 publications

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations

Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation

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