Role of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 in<i>Pseudomonas aeruginosa</i>

Manohar, Prasanth; Shanthini, Thamaraiselvan; Ra, Philip; Ramkumar, Srikrishnan Rajendran Sri; Kale, Mamata; Nachimuthu, Ramesh

doi:10.1101/341826

Cited by 3 publications

(2 citation statements)

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“…Due to the increased resistance to antibiotics in the treatment of infections caused by biofilm-forming strains of P. aeruginosa, the treatment of infections becomes very difficult (Jennings et al, 2015). In the study conducted by Manohar et al (2018), 8 of 24 P. aeruginosa strains isolated from various clinical cases were found positive for biofilm production. Furthermore, in conclusion of the conducted antibiogram test 63% of the strains were found to be resistant to gentamicin, 71% to ciprofloxacin, and 50% to meropenem.…”

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confidence: 99%

Türkiye ve Romanya kökenli Pseudomonas aeruginosa izolatlarının antibiyotik dirençlerinin, biyofilm oluşumu ve genotiplerinin araştırılması

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et al. 2022

Etlik Veteriner Mikrobiyoloji Dergisi

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Pseudomonas aeruginosa is frequently isolated from various disease and nosocomial infections. It is clinically important because of its multiple antibiotic resistance and biofilm production. The aims of this study were to determine the production of biofilm and to make antibiotyping and genotyping of P. aeruginosa strains isolated from bronchial aspirate samples of dogs with pneumonia in Turkey and Romania. For this purpose, biofilm properties of 10 Turkish and 10 Romanian isolates were determined by Congo red agar method. The resistance profiles of the isolates were determined by Kirby-Bauer Disk Diffusion Method. The antibiotyping was performed according to the resistance profiles of the isolates. Genotypical similarities were determined by RAPD-PCR. In terms of biofilm production, all of the isolates examined in this study were found to be positive. In consequence of the antibiograms, the resistance rates against enrofloxacin, ceftriaxone, amoxicillin + clavulanic acid, ciprofloxacin, meropenem, colistin, gentamicin and azithromycin were stated as 30%, 70%, 100%, 40%, 30%, 10%, 10%, 100% for Turkish isolates and 30%, 60%, 100%, 30%, 10%, 0%, 10%, 100% for Romanian isolates, respectively. As a result of antibiotyping, similarities of the isolates from Turkey and Romania were determined to be between 86-100%. Phylogenotypical similarities of the isolates from Turkey and Romania were determined to be between 47-96%. It was concluded that antibiotic resistances of isolates were high and resistance against antibiotics used in the treatment could be formed due to biofilm production.

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Section: Discussionmentioning

confidence: 99%

Türkiye ve Romanya kökenli Pseudomonas aeruginosa izolatlarının antibiyotik dirençlerinin, biyofilm oluşumu ve genotiplerinin araştırılması

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et al. 2022

Etlik Veteriner Mikrobiyoloji Dergisi

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“…, PA0757, PA2070 and PA5033 gene entities responsible for bio lm-related antibiotic resistance in isolates were investigated with method reported byManohar et al (2018). After imaging, 1130, 1011, 1355, 1320 bp bands were evaluated as positive for PA0756, PA0757, PA2070 and PA5033 gene entities, respectively.…”

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The comparative evaluation of biofilm production and antibiotic resistance in Pseudomonas aeruginosa isolates

Ekselli,

Çiftci

2024

Preprint

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In this study, it was aimed to investigate the biofilm formation, antibiotic resistance profiles, the genes which play roles for biofilm and antibiotic resistances of 48 Pseudomonas aeruginosa isolates isolated from different clinical samples. The identifications of the P.aeruginosa isolates were confirmed with PCR. The antibiotic resistances against 8 antibiotics were determined by Kirby-Bauer Disc Diffusion method. The biofilm formations of the isolates were investigated in vitro with Congo Red Agar, Modified Tube Adherence (Christensen) and Micro plate Methods. The isolates which were found as positive with three methods were genotyped with RAPD-PCR. pelA, lasI, lasR, rhlI, rhlR,PA0756, PA0757, PA2070 and PA5033 genes were investigated with using PCR. The antibiotic resistance rates of the isolates among enrofloxacine, ceftriaxone, amoxicilline-clavulanic acid, ciprofloxacin, meropenem, colistin, gentamycin, and azithromycin were found as 16.67%, 60.42%, 100%, 10.42%, 29.17%, 4.17%, 12.5% and %97.9, respectively. The biofilm formation ratios were determined as 83,33% with CRA, 93.75% with Christensen method and 64.58% with micro plate method. pelA, lasI, rhlI and rhlR were carried by all the isolates. lasR gene was found in %97.92 of the isolates. Fortysix (95.83%) of the isolates carried PA0756 and PA0757. Fortyfive (93.75%) of the isolates were found as positive for PA2070 and PA5033 genes. The phylogenetic relations of the biofilm positive isolates were calculated as 56–93%. The biofilm formation and multiple antibiotic resistance rates were found as high in the clinical isolates of P. aeruginosa. The widespread and advanced studies thought to be performed for the lightening of this situation and biofilm related antibiotic resistances.

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Investigating Biofilms: Advanced Methods for Comprehending Microbial Behavior and Antibiotic Resistance

Almatroudi

2024

Front. Biosci. (Landmark Ed)

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Biofilms, which consist of microorganisms enclosed in an extracellular polymeric material (EPS), hold immense importance in the fields of environmental research, industry, and medicine. They play a significant role in ecosystem dynamics and stability, but they also pose issues such as biofouling, corrosion, and pollution. Biofilms in medical environments are linked to persistent infections and elevated healthcare expenses. The EPS matrix plays a crucial role in maintaining the structural integrity and antibiotic resistance of these structures. The research primarily investigates the role of the EPS matrix in facilitating horizontal gene transfer among biofilm communities, with a particular emphasis on EPS and its impact on this process. The process is recognized as a pivotal mechanism in the emergence of antibiotic resistance, underscoring the crucial function of EPS in the dynamics of biofilms. The analysis also highlights the significant financial constraints caused by biofilms in several industries. Biofilm-associated infections in the healthcare sector result in escalated treatment expenses and extended hospitalization periods. In an industrial context, biofilms have a role in increasing maintenance expenses and product contamination, emphasizing the need for efficient management solutions. This review presents the most recent progress in biofilm research, emphasizing the utilization of sophisticated imaging tools and molecular methodologies. In addition to conventional imaging techniques, the research explores the utilization of sophisticated molecular tools, such as DNA and RNA sequencing, in conjunction with proteomics. These approaches are essential for assessing the genetic and metabolic mechanisms that regulate biofilm development and antibiotic resistance. The review underscores the significance of employing an interdisciplinary methodology in the study of biofilms. By incorporating a range of approaches, such as sophisticated imaging and molecular analysis, a comprehensive understanding of biofilm dynamics may be achieved. This approach also opens up possibilities for developing novel solutions to address the negative impacts of biofilms on health, industry, and the environment.

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Role of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 inPseudomonas aeruginosa

Cited by 3 publications

References 19 publications

Türkiye ve Romanya kökenli Pseudomonas aeruginosa izolatlarının antibiyotik dirençlerinin, biyofilm oluşumu ve genotiplerinin araştırılması

Türkiye ve Romanya kökenli Pseudomonas aeruginosa izolatlarının antibiyotik dirençlerinin, biyofilm oluşumu ve genotiplerinin araştırılması

The comparative evaluation of biofilm production and antibiotic resistance in Pseudomonas aeruginosa isolates

Investigating Biofilms: Advanced Methods for Comprehending Microbial Behavior and Antibiotic Resistance

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