Role of biomechanics in vascularization of tissue-engineered bones

Mokhtari-Jafari, Fatemeh; Amoabediny, Ghassem

doi:10.1016/j.jbiomech.2020.109920

Cited by 18 publications

(13 citation statements)

References 147 publications

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“…The biological manifestations of porous scaffolds are closely related to the nutritional diffusion and metabolism inside the scaffolds. Similar to the findings by Mokhtari-Jafari et al (2020) , computational modeling and multiscale systems biology elucidating the contributions of endothelial cell proliferation and migration. The simulated results suggested that the permeability of the GI group (pore size increases from the center to the perimeter) was better than that of the other two groups under any conditions, which was consistent with the in vivo test results.…”

Section: Discussionsupporting

confidence: 81%

“…For bioactive scaffolds, it is of great significance to possess the appropriate biomechanical properties to adapt to natural bones ( Turnbull et al, 2018 ), excessive mechanical properties will lead to stress shielding ( Sola et al, 2016 ), while too small mechanical properties cannot maintain the space maintenance of scaffolds, resulting in bone resorption ( Kadkhodapour et al, 2015 ; Vaquette et al, 2021 ). Moreover, biomechanical properties also influence cell behaviors, modulate local environment ( Mokhtari-Jafari et al, 2020 ). According to the FEA and compression tests in this study, although the compressive strength and peak stress of the GII were significantly higher than those of the other two groups, the elastic modulus of the three groups were all close to those of cancellous bone and cortical bone (0.5–20 GPa) ( Parthasarathy et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

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Biomechanical Effects of 3D-Printed Bioceramic Scaffolds With Porous Gradient Structures on the Regeneration of Alveolar Bone Defect: A Comprehensive Study

Yang

Wang

Gao

et al. 2022

Front. Bioeng. Biotechnol.

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In the repair of alveolar bone defect, the microstructure of bone graft scaffolds is pivotal for their biological and biomechanical properties. However, it is currently controversial whether gradient structures perform better in biology and biomechanics than homogeneous structures when considering microstructural design. In this research, bioactive ceramic scaffolds with different porous gradient structures were designed and fabricated by 3D printing technology. Compression test, finite element analysis (FEA) revealed statistically significant differences in the biomechanical properties of three types of scaffolds. The mechanical properties of scaffolds approached the natural cancellous bone, and scaffolds with pore size decreased from the center to the perimeter (GII) had superior mechanical properties among the three groups. While in the simulation of Computational Fluid Dynamics (CFD), scaffolds with pore size increased from the center to the perimeter (GI) possessed the best permeability and largest flow velocity. Scaffolds were cultured in vitro with rBMSC or implanted in vivo for 4 or 8 weeks. Porous ceramics showed excellent biocompatibility. Results of in vivo were analysed by using micro-CT, concentric rings and VG staining. The GI was superior to the other groups with respect to osteogenicity. The Un (uniformed pore size) was slightly inferior to the GII. The concentric rings analysis demonstrated that the new bone in the GI was distributed in the periphery of defect area, whereas the GII was distributed in the center region. This study offers basic strategies and concepts for future design and development of scaffolds for the clinical restoration of alveolar bone defect.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Biomechanical Effects of 3D-Printed Bioceramic Scaffolds With Porous Gradient Structures on the Regeneration of Alveolar Bone Defect: A Comprehensive Study

Yang

Wang

Gao

et al. 2022

Front. Bioeng. Biotechnol.

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“…In the existing literature, the fate of the transplanted ADSCs remains unelucidated. The survival of transplanted cells depends on multiple composite factors in the nerve microenvironment, including new vessel formation, energy metabolism and inflammatory regulation ( Chen et al, 2013 ; Gao et al, 2018 ; Mokhtari-Jafari et al, 2020 ). Therefore it could be extremely hard to tell the exact number of cells left in the transplanted site.…”

Section: Discussionmentioning

confidence: 99%

“…Over the past years, Schwann cell (SC) transplantation has emerged as possible strategy to assist in the clinical treatment after PNIs (Guénard et al, 1992). On the one hand, SCs produce neurotrophic factors, such as brain derived neurotrophic factor (BDNF), glial-cell derived neurotrophic factor (GDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3), which facilitate the axonal regeneration and neurite outgrowth (Makwana and Raivich, 2005). On the other hand, SCs wrap axons and are crucial for the formation of myelin sheath (Salzer, 2015).…”

Section: Introductionmentioning

confidence: 99%

Tacrolimus-Induced Neurotrophic Differentiation of Adipose-Derived Stem Cells as Novel Therapeutic Method for Peripheral Nerve Injury

Yao

Yan²,

Li³

et al. 2021

Front. Cell. Neurosci.

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Peripheral nerve injuries (PNIs) are frequent traumatic injuries across the globe. Severe PNIs result in irreversible loss of axons and myelin sheaths and disability of motor and sensory function. Schwann cells can secrete neurotrophic factors and myelinate the injured axons to repair PNIs. However, Schwann cells are hard to harvest and expand in vitro, which limit their clinical use. Adipose-derived stem cells (ADSCs) are easily accessible and have the potential to acquire neurotrophic phenotype under the induction of an established protocol. It has been noticed that Tacrolimus/FK506 promotes peripheral nerve regeneration, despite the mechanism of its pro-neurogenic capacity remains undefined. Herein, we investigated the neurotrophic capacity of ADSCs under the stimulation of tacrolimus. ADSCs were cultured in the induction medium for 18 days to differentiate along the glial lineage and were subjected to FK506 stimulation for the last 3 days. We discovered that FK506 greatly enhanced the neurotrophic phenotype of ADSCs which potentiated the nerve regeneration in a crush injury model. This work explored the novel application of FK506 synergized with ADSCs and thus shed promising light on the treatment of severe PNIs.

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“…This ability could mechanically stimulate the cells cultivated on the scaffold, influencing tissue replacement during cell seeding. In vitro maturation and differentiation of the cells before the implantation might be thus affected, speeding up the healing process and improving the functionality of the scaffold soon after the implantation [ 24 , 25 , 26 ]. The possibility to deform the implant in a noninvasive way will also allow controlled perfusion of the cultivation medium under in vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Superporous Poly(2-hydroxyethyl methacrylate) Hydrogel Scaffolds for Bone Tissue Engineering

et al. 2021

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Magnetic maghemite (γ-Fe2O3) nanoparticles obtained by a coprecipitation of iron chlorides were dispersed in superporous poly(2-hydroxyethyl methacrylate) scaffolds containing continuous pores prepared by the polymerization of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) in the presence of ammonium oxalate porogen. The scaffolds were thoroughly characterized by scanning electron microscopy (SEM), vibrating sample magnetometry, FTIR spectroscopy, and mechanical testing in terms of chemical composition, magnetization, and mechanical properties. While the SEM microscopy confirmed that the hydrogels contained communicating pores with a length of ≤2 mm and thickness of ≤400 μm, the SEM/EDX microanalysis documented the presence of γ-Fe2O3 nanoparticles in the polymer matrix. The saturation magnetization of the magnetic hydrogel reached 2.04 Am2/kg, which corresponded to 3.7 wt.% of maghemite in the scaffold; the shape of the hysteresis loop and coercivity parameters suggested the superparamagnetic nature of the hydrogel. The highest toughness and compressive modulus were observed with γ-Fe2O3-loaded PHEMA hydrogels. Finally, the cell seeding experiments with the human SAOS-2 cell line showed a rather mediocre cell colonization on the PHEMA-based hydrogel scaffolds; however, the incorporation of γ-Fe2O3 nanoparticles into the hydrogel improved the cell adhesion significantly. This could make this composite a promising material for bone tissue engineering.

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Role of biomechanics in vascularization of tissue-engineered bones

Cited by 18 publications

References 147 publications

Biomechanical Effects of 3D-Printed Bioceramic Scaffolds With Porous Gradient Structures on the Regeneration of Alveolar Bone Defect: A Comprehensive Study

Biomechanical Effects of 3D-Printed Bioceramic Scaffolds With Porous Gradient Structures on the Regeneration of Alveolar Bone Defect: A Comprehensive Study

Tacrolimus-Induced Neurotrophic Differentiation of Adipose-Derived Stem Cells as Novel Therapeutic Method for Peripheral Nerve Injury

Magnetic Superporous Poly(2-hydroxyethyl methacrylate) Hydrogel Scaffolds for Bone Tissue Engineering

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