Role of Biotechnology for Protection of Endangered Medicinal Plants

Tasheva, Krasimira; Kosturkova, Georgina

doi:10.5772/55024

Cited by 13 publications

(14 citation statements)

References 116 publications

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“…Cultivation of endangered medicinal plants under controlled conditions represents a promising protective approach (Tasheva and Kosturkova, 2013). For some of the stages which make up the process of domestication of wild-growing plant species, in vitro techniques can be applied, for example in selection breeding for genetic improvement or storage of plant materials (Bohr, 1997; Franz, 1991).…”

Section: Approaches For Resupply Of Pharmacologically Active Plantmentioning

confidence: 99%

“…On a commercial scale it has developed into a multi-billion-dollar industry and offers important advantages over the conventional propagation of plants, like production of large numbers of genetically homogenous plants year-round in a comparatively short time, disease-free propagules, and greatly enhanced multiplication rates (Debnath et al, 2006). Micropropagation has become a standard method of plant production for many species of economic value, such as plants of agricultural, ornamental, vegetable, and forestry importance (Debnath et al, 2006; Read, 2007; Tasheva and Kosturkova, 2013). In vitro propagation certainly also holds significant potential for the mass production of medicinal plants and numerous protocols for micropropagation of a huge number of medicinally used species have been developed up to date (Afolayan and Adebola, 2004; Chaturvedi et al, 2007; Debnath et al, 2006; Rout et al, 2000; Sarasan et al, 2011; Tripathi and Tripathi, 2003; Wawrosch, 2010).…”

Section: Approaches For Resupply Of Pharmacologically Active Plantmentioning

confidence: 99%

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Discovery and resupply of pharmacologically active plant-derived natural products: A review

Atanasov

Waltenberger

Pferschy‐Wenzig

et al. 2015

Biotechnology Advances

2,276

1,385

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Medicinal plants have historically proven their value as a source of molecules with therapeutic potential, and nowadays still represent an important pool for the identification of novel drug leads. In the past decades, pharmaceutical industry focused mainly on libraries of synthetic compounds as drug discovery source. They are comparably easy to produce and resupply, and demonstrate good compatibility with established high throughput screening (HTS) platforms. However, at the same time there has been a declining trend in the number of new drugs reaching the market, raising renewed scientific interest in drug discovery from natural sources, despite of its known challenges. In this survey, a brief outline of historical development is provided together with a comprehensive overview of used approaches and recent developments relevant to plant-derived natural product drug discovery. Associated challenges and major strengths of natural product-based drug discovery are critically discussed. A snapshot of the advanced plant-derived natural products that are currently in actively recruiting clinical trials is also presented. Importantly, the transition of a natural compound from a “screening hit” through a “drug lead” to a “marketed drug” is associated with increasingly challenging demands for compound amount, which often cannot be met by re-isolation from the respective plant sources. In this regard, existing alternatives for resupply are also discussed, including different biotechnology approaches and total organic synthesis.While the intrinsic complexity of natural product-based drug discovery necessitates highly integrated interdisciplinary approaches, the reviewed scientific developments, recent technological advances, and research trends clearly indicate that natural products will be among the most important sources of new drugs also in the future.

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Section: Approaches For Resupply Of Pharmacologically Active Plantmentioning

confidence: 99%

Section: Approaches For Resupply Of Pharmacologically Active Plantmentioning

confidence: 99%

Discovery and resupply of pharmacologically active plant-derived natural products: A review

Atanasov

Waltenberger

Pferschy‐Wenzig

et al. 2015

Biotechnology Advances

2,276

1,385

View full text Add to dashboard Cite

show abstract

“…The alternative solution to these restrictions may be production of plant biomass of the endangered species applying the biotechnological methods. Rapid production of homogeneous and renewable biomass capable of biosynthesis of substances with the pharmacological activity, and most importantly the possibility of strict control of this process, indicate the opportunity for wider application of the biotechnological methods in the pharmaceutical and cosmetic industry [28,29].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition of Essential Oils from Rare and Endangered Species—Eryngium maritimum L. and E. alpinum L.

Kikowska

Kalemba

Długaszewska

et al. 2020

Plants

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In the present study, the essential oils obtained by hydrodistillation of the organs of Eryngium maritimum and E. alpinum were analyzed by GC-FID-MS. The dominant constituents assessed in the essential oil of E. maritimum were germacrene D (45.2%) in the fruits; hexadecanoic acid (18.5%), menthol (16.8%), and menthone (10.9%) in the roots; 2,3,4-trimethylbenzaldehyde (11.3%) and germacrene D (10.5%) in the leaves; 2,3,4-trimethylbenzaldehyde (11.3%) in the shoot culture. In the case of E. alpinum, the main components of the leaf oil were: β-elemenone (10.3%), germacrone (5.8%), two selinadienes (7.1% and 6.7%), and 1,8-cineole (5.3%), which were not found in the oil from shoot culture, whereas the shoot culture oil was dominated by hexadecanoic acid (15.5%), spathulenol (7.5%), (E)-β-farnesene (4.9%), germacra-4(15),5,10(14)-trien-1α-ol (4.7%), and falcarinol (4.3%). The essential oils obtained from E. maritimum fruits and leaves of the intact plants, measured by the broth microdilution method, were the most active against T. mentagophytes and S. aureus. Moreover, the essential oil of leaves had the moderate activity against C. albicans and E. coli. The results showed that the chemical compositions of the essential oils differed decidedly between the two studied species and between the organs. Furthermore, the essential oil of E. maritimum may play an important role as antimicrobial agent.

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“…It has now been classified as a protected plant in China [ 1 ]. Similarly, many other Gentiana species have become endangered species [ 9 ]. Studies have suggested that the chromosome number of Gentiana triflora , Gentiana scabra , Gentiana manshurica , and other herbs containing gentiopicroside, is 2 n = 26, while that of Gentiana lutea and Gentiana punctata is 2 n = 40 [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

De Novo Assembly and Characterization of the Transcriptome of the Chinese Medicinal Herb, Gentiana rigescens

Zhang

Allan

et al. 2015

IJMS

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Gentiana rigescens is an important medicinal herb in China. The main validated medicinal component gentiopicroside is synthesized in shoots, but is mainly found in the plant’s roots. The gentiopicroside biosynthetic pathway and its regulatory control remain to be elucidated. Genome resources of gentian are limited. Next-generation sequencing (NGS) technologies can aid in supplying global gene expression profiles. In this study we present sequence and transcript abundance data for the root and leaf transcriptome of G. rigescens, obtained using the Illumina Hiseq2000. Over fifty million clean reads were obtained from leaf and root libraries. This yields 76,717 unigenes with an average length of 753 bp. Among these, 33,855 unigenes were identified as putative homologs of annotated sequences in public protein and nucleotide databases. Digital abundance analysis identified 3306 unigenes differentially enriched between leaf and root. Unigenes found in both tissues were categorized according to their putative functional categories. Of the differentially expressed genes, over 130 were annotated as related to terpenoid biosynthesis. This work is the first study of global transcriptome analyses in gentian. These sequences and putative functional data comprise a resource for future investigation of terpenoid biosynthesis in Gentianaceae species and annotation of the gentiopicroside biosynthetic pathway and its regulatory mechanisms.

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Role of Biotechnology for Protection of Endangered Medicinal Plants

Cited by 13 publications

References 116 publications

Discovery and resupply of pharmacologically active plant-derived natural products: A review

Discovery and resupply of pharmacologically active plant-derived natural products: A review

Chemical Composition of Essential Oils from Rare and Endangered Species—Eryngium maritimum L. and E. alpinum L.

De Novo Assembly and Characterization of the Transcriptome of the Chinese Medicinal Herb, Gentiana rigescens

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