Role of C3 Cleavage in Monocyte Activation During Extracorporeal Circulation

Rinder, Christine S.; Rinder, Henry M.; Johnson, Kirk W.; Smith, Michael J.; Lee, David L.; Tracey, Jayne B.; Polack, Glenda; Higgins, Paul J.; Yeh, Chiu‐Li; Smith, Brian R.

doi:10.1161/01.cir.100.5.553

Cited by 53 publications

(30 citation statements)

References 27 publications

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“…Previous work has described how circulating monocytes may exacerbate pro-inflammatory reactions immediately following cardiac surgery and so potentially delay patient's recovery [6,15,16]. In this study, we demonstrate that monocytes also possess anti-inflammatory and haemoglobin scavenging properties at a later phase of recovery following coronary surgery and that this response is influenced by using CPB (Fig.…”

Section: Discussionsupporting

confidence: 57%

“…Therefore, it is likely that other factors besides shedding contribute to the transient decline and subsequent peak in monocyte CD163 expression. Monocytes are activated following CABG surgery and typically express adhesion molecules such as CD11b and CD62L [15,16]. It is thus possible that adhesion to the extracorporeal circuit or tissue extravasation may lead to a loss of CD163-bearing cells during cardiac surgery.…”

Section: Discussionmentioning

confidence: 99%

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Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery

Philippidis¹,

Athanasiou²,

Nadra³

et al. 2010

European Journal of Cardio-Thoracic Surgery

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Coronary artery bypass surgery induces monocyte-associated anti-inflammatory and immunoregulatory responses. There was no significant difference in haemolysis although the effect on monocyte CD163 and plasma sCD163 concentration was significantly different between the two groups. Compensation for varying pro-inflammatory and haemolytic insults may explain the differences observed. Therapeutic strategies for inducing such desirable circulating monocytes may potentially improve surgical outcome and patient recovery.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery

Philippidis¹,

Athanasiou²,

Nadra³

et al. 2010

European Journal of Cardio-Thoracic Surgery

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“…In line with this theory, researchers have elucidated roles for complement in enhancing PGA formation in human whole blood using extracorporeal circuits that simulate coronary artery bypass and ex vivo flow cytometry assays . Rinder et al .…”

Section: Properdin and Thromboinflammationmentioning

confidence: 99%

Properdin: a tightly regulated critical inflammatory modulator

Blatt

Pathan

Ferreira

2016

Immunological Reviews

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SummaryThe complement alternative pathway is a powerful arm of the innate immune system that enhances diverse inflammatory responses in the human host. Key to the effects of the alternative pathway is properdin, a serum glycoprotein that can both initiate and positively regulate alternative pathway activity. Properdin is produced by many different leukocyte subsets and circulates as cyclic oligomers of monomeric subunits. While the formation of non‐physiological aggregates in purified properdin preparations and the presence of potential properdin inhibitors in serum have complicated studies of its function, properdin has, regardless, emerged as a key player in various inflammatory disease models. Here, we review basic properdin biology, emphasizing the major hurdles that have complicated the interpretation of results from properdin‐centered studies. In addition, we elaborate on an emerging role for properdin in thromboinflammation and discuss the potential utility of properdin inhibitors as long‐term therapeutic options to treat diseases marked by increased formation of platelet/granulocyte aggregates. Finally, we describe the interplay between properdin and the alternative pathway negative regulator, Factor H, and how aiming to understand these interactions can provide scientists with the most effective ways to manipulate alternative pathway activation in complex systems.

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“…In the childhood, the functions of a majority of organs (such as pulmonary circulation) are immature and thus children are susceptible to severe complications of CPB (11). In the CPB induced systemic inflammatory response syndrome (SIRS), the circulating PBMC are not only the effector cells but the cells regulating the inflammatory reaction (12). Thus, investigating the effect of CPB on the proteomic profiling in PBMC is helpful to elucidate the role of PBMC in the CPB induced SIRS and to identify the inflammation related signal trans duction proteins involved in the SIRS.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cardiopulmonary Bypass on Annexin A1 Expression in Peripheral Blood Mononuclear Cells of Children with Congenital Heart Disease

Zhou¹,

Hong²,

Liu³

et al. 2012

Journal of Medical Biochemistry

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Summary: This study aimed to investigate the effect of cardiopulmonary bypass (CPB) on Annexin A1 expression in the peripheral blood mononuclear cells (PBMCs) of children with congenital heart disease (CHD). A total of 30 children receiving CPB for interventricular septal defect were included. Peripheral blood was collected before and after CPB. PBMCs were collected by density gradient centrifugation. Protein extra ction was performed by lysis and subjected to 2D-QUANT for protein quantitation. Isoelectric focusing electrophoresis (IEF) was carried out followed by gel image analysis. Protein spots with a difference in expression of >1.5 fold were collected as candidate proteins which were subjected to mass spectrometry for the identification of diffe rentially expressed proteins. Western blot assay was emplo yed to confirm the expressions of target proteins. Peripheral blood collected at two time points was subjected to two-dimensional electrophoresis, and a total of 12 differentially expressed proteins were identified. Of them, 5 proteins had decreased expression before CPB (T0) but their expressions increased after CPB (T1); the remaining 7 proteins had in cre ased expres sions before CPB but their expressions re du ced after CPB. One of these differentially expres sed proteins was Annexin A1. Western blot assay confirmed that Annexin A1 expression began to increase at 0.5 h after CPB, and the increase of Annexin A1 was more obvious after CPB. Our findings primarily indicate the potential mechanism underlying the role of PBMC in inflammatory response following CPB, and provide a target for the prevention and control of post-CPB systemic inflammatory response syndrome (SIRS).Keywords: annexin A1, cardiopulmonary bypass, peripheral blood mononuclear cells, congenital heart disease Kratak sadr`aj: Svrha studije bila je da se istra`i uticaj kardiopulmonarnog bajpasa (KPB) na ekspresiju aneksina A1 u mononuklearnim }elijama periferne krvi (M]PK) dece sa uro|enim sr~anim oboljenjima (SO). Istra`ivanjem je obuhva}eno ukupno 30 dece kod koje je zbog interventrikularnog septalnog defekta izveden kardiopulmonarni bajpas. Uzorci periferne krvi uzeti su pre i posle zahvata. M]PK su sakupljene centrifugiranjem u gradijentu gustine. Proteini su ekstra hovani lizom a kvantitacija proteina obavljena je metodom 2D-QUANT. Izvr{ena je elektroforeza izoelektri~nim fokusiranjem (EIF) a potom analiza gel image. Proteinske ta~ke sa razlikom u ekspresiji >1,5 puta uzete su kao proteini kandidati, koji su podvrgnuti masenoj spektrometriji radi identifikovanja razli~ite ekspresije proteina. Za potvr|ivanje ekspresija ciljnih proteina upotrebljen je test Western blot. Uzorci periferne krvi uzeti su dva puta u odre|enom vremenskom razmaku i podvrgnuti dvodimenzionalnoj elektroforezi te je identifikovano ukupno 12 diferencijalno izra`enih proteina. Od toga je 5 proteina imalo smanjenu ekspresiju pre KPB (T0), ali se njihova ekspresija posle KPB pove}ala (T1); preostalih 7 proteina imalo je pove}anu ekspresiju pre KPB, ali se njihova ekspr...

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Role of C3 Cleavage in Monocyte Activation During Extracorporeal Circulation

Cited by 53 publications

References 27 publications

Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery

Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery

Properdin: a tightly regulated critical inflammatory modulator

Effect of Cardiopulmonary Bypass on Annexin A1 Expression in Peripheral Blood Mononuclear Cells of Children with Congenital Heart Disease

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