Role of calcium in gamma interferon induction: inhibition by calcium entry blockers

Dianzani, F.; Capobianchi, Maria Rosaria; Facchini, J.

doi:10.1128/jvi.50.3.964-965.1984

Cited by 15 publications

(2 citation statements)

References 7 publications

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“…Our study provides significant insight into the relationship between calcium signaling and IFN-γ translation in NK cells. Indeed, several reports show that IFN-γ production by immune cells depends on calcium signaling [ 9 ]. For instance, production of IFN-γ by cord blood mononuclear cells requires uptake of extracellular calcium [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

25KDa branched polyethylenimine increases interferon-γ production in natural killer cells via improving translation efficiency

Park

Lee

et al. 2023

Cell Commun Signal

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Background Ex vivo cultivation is a promising strategy for increasing the number of NK cells and enhancing their antitumor activity prior to clinical application. Recent studies show that stimulation with 25KDa branched polyethylenimine (25KbPEI) generates NK cells with enhanced antitumor activity. To better understand how 25KbPEI primes NK cells, we explored the mechanism underlying increase in production of IFN-γ. Methods Chemical priming was performed on NK-92MI cells by incubating them with 5 μg/ml of 25KbPEI. The production of IFN-γ was evaluated by RT-qPCR, ELISA, and Flow cytometry. By evaluating the effect of pharmacological inhibition of ERK/mTOR-eIF4E signaling pathways on IFN-γ translation, the function of these signaling pathways in IFN-γ translation was examined. To comprehend the level of 25KbPEI activity on immune-related components in NK cells, RNA sequencing and proteomics analyses were conducted. Results 25KbPEI enhances the production of IFN-γ by NK cells without transcriptional activation. Activation of ERK and mTOR signaling pathways was found to be associated with 25KbPEI-mediated calcium influx in NK cells. The activation of ERK/mTOR signaling was linked to the phosphorylation of 4E-BP1, which resulted in the activation of translation initiation complex and subsequent IFN-γ translation. Analysis of RNA sequencing and proteomics data revealed that the activity of 25KbPEI to improve translation efficiency in NK cells could be extended to additional immune-related molecules. Conclusions This study provides substantial insight into the process by which 25KbPEI primes NK cells. Our data demonstrated that the 25KbPEI mediated activation of ERK/mTOR signaling and subsequent stimulation of eIF4E is the primary mechanism by which the chemical stimulates translation of IFN-γ in NK cells.

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Section: Discussionmentioning

confidence: 99%

25KDa branched polyethylenimine increases interferon-γ production in natural killer cells via improving translation efficiency

Park

Lee

et al. 2023

Cell Commun Signal

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“…Verapamil has been shown to inhibit macrophage production of INF-y. 29 It is possible that the elevation of progesterone production was a result ofverapamil-induced suppression of IFN-y secretion by the macrophage. However, a comparison of results in Fig.…”

Section: -mentioning

confidence: 99%

The Effect of Peritoneal Macrophage‐Derived Factor(s) on Ovarian Progesterone Secretion and LH Receptors: The Role of Calcium

Chen

Lane

Doody

et al. 1992

American J Rep Immunol

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Macrophages and their secretory products, cytokines, play an integral role in many reproductive processes. In this study we examined the effect of conditioned media from cultured human peritoneal macrophages on progesterone production by granulosa cells and the role of calcium in this process. Macrophages were pretreated with various concentrations of a calcium channel blocker (verapamil) or a calcium ionophore (A23187). Macrophage-conditioned media (MCM) or cell-free media that contained calcium channel modifiers were added at three dose levels to cultured porcine granulosa cells. Progesterone production and LH receptor content were determined. Macrophage-conditioned media alone elevated basal progesterone production, but significantly attenuated granulosa cell LH receptor content. These effects were neither potentiated nor suppressed by pretreating macrophages with verapamil. However, production of the LH receptor lowering factor(s) appeared to be suppressed by calcium ionophore. We conclude that (1) one or more factors produced by macrophages have a net stimulatory effect on basal progesterone production and these factor(s) may not be calcium-dependent and (2) macrophage-derived secretory products reduce granulosa cell LH receptor content. The production of these factor(s) may be calcium-dependent.

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Human B cell activation: Selective sensitivity of the early stages to calcium channel‐blocking drugs

et al. 1986

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The importance of Ca2+ in the early events of lymphocyte activation has been suggested by several studies. We examined the effect of calcium channel-blocking drugs (verapamil and nitrendipine) on the progression of human B cells through their activation cycle. Our results show that these drugs suppress the anti-mu-induced human B cell proliferation and interfere with the early events of the B cell activation in a dose-dependent fashion. This suppression correlates with a marked decrease in anti-mu-induced 45Ca2+ uptake. Calcium channel-blocking drugs inhibit the anti-mu-induced uridine incorporation and the appearance of the activation marker defined by the 4F2 monoclonal antibody. Calcium channel-blocking drugs also inhibit B cell proliferation induced by the costimulation with anti-mu antibody and B cell growth factor (BCGF). However, this inhibition takes place at the early (anti-mu-dependent) stage of B cell activation: the BCGF-dependent proliferation of in vitro anti-mu-activated B cells is only marginally inhibited. Finally the proliferation of Epstein-Barr virus-infected B cell lines is resistant to the effect of calcium channel-blocking drugs.

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Role of calcium in gamma interferon induction: inhibition by calcium entry blockers

Cited by 15 publications

References 7 publications

25KDa branched polyethylenimine increases interferon-γ production in natural killer cells via improving translation efficiency

25KDa branched polyethylenimine increases interferon-γ production in natural killer cells via improving translation efficiency

The Effect of Peritoneal Macrophage‐Derived Factor(s) on Ovarian Progesterone Secretion and LH Receptors: The Role of Calcium

Human B cell activation: Selective sensitivity of the early stages to calcium channel‐blocking drugs

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