Role of calcium in nitric oxide-induced programmed cell death in tobacco protoplasts

Wang, Y.; Lin, Jer-Sheng; Wang, G. X.

doi:10.1007/s10535-010-0083-2

Cited by 16 publications

(7 citation statements)

References 38 publications

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“…Superoxide is then transformed to H 2 O 2 by superoxide dismutase (Kraus and Fletcher 1994). SNP is a NO donor used in plant experimental systems (Wang et al 2010). 0.5 mM SNP can produce 2 μM NO (Delledonne et al 1998).…”

Section: Resultsmentioning

confidence: 99%

Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis

Zhou¹,

Li²,

Zhang³

et al. 2012

Biologia plant.

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Vegetative growth and reproductive growth strongly competes with each other during panicle development in litchi (Litchi chinensis Sonn.). We herein investigated the roles of hydrogen peroxide and nitric oxide in the competition between growth of rudimentary leaves and panicle development. The results show that the chilling-induced flowering increased H 2 O 2 and NO contents in the mixed buds. Treatments with sodium nitroprusside (SNP), the NO donor, and methyl viologen dichloride hydrate (MV), the superoxide generator, increased NO and H 2 O 2 contents in the mixed buds. MV and SNP treatments promoted abscission of rudimentary leaves and encouraged panicle development before or at the stage of panicle emergence. The nitric oxide synthase inhibitor N ω -nitro-L-arginine methyl ester (L-NAME) and the H 2 O 2 trapper dimethylthiourea (DMTU) inhibited a chilling-induced flowering. SNP promoted the expression of litchi LEAFY homolog (LcLFY). These promotive effects were suppressed by the NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl3-oxide (PTIO) and the H 2 O 2 trapper, DMTU. The results suggest that H 2 O 2 and NO promote reproductive growth by inhibiting the growth of rudimentary leaves as well as by promoting the expression of the flower related gene, LcLFY.Additional key words: flowering, LEAFY, methyl viologen, sodium nitroprusside.

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Section: Resultsmentioning

confidence: 99%

Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis

Zhou¹,

Li²,

Zhang³

et al. 2012

Biologia plant.

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“…Stress responses are triggered by the stresses that the cells suffered. [ 24 , 75 , 76 ]. The occurrence of stress response affects a series of cellular activities, and the overall performance is the hyperpolarization of TMP [ 2 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Transmembrane potential, an indicator in situ reporting cellular senescence and stress response in plant tissues

Liu

Xue

2023

Plant Methods

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Background Plant cells usually sustain a stable membrane potential due to influx and/or efflux of charged ions across plasma membrane. With the growth and development of plants, different tissues and cells undergo systemic or local programmed decline. Whether the membrane potential of plasma membrane could report senescence signal of plant tissues and cells is unclear. Results We applied a maneuverable transmembrane potential (TMP) detection method with patch-clamp setup to examine the senescence signal of leaf tissue cells in situ over the whole life cycle in Arabidopsis thaliana. The data showed that the TMPs of plant tissues and cells were varied at different growth stages, and the change of TMP was higher at the vegetative growth stage than at the reproductive stage of plant growth. The distinct change of TMP was detectable between the normal and the senescent tissues and cells in several plant species. Moreover, diverse abiotic stimuli, such as heat stress, hyperpolarized the TMP in a short time, followed by depolarized membrane potential with the senescence occurring. We further examined the TMP of plant chloroplasts, which also indicates the senescence signal in organelles. Conclusions This convenient TMP detection method can report the senescence signal of plant tissues and cells, and can also indicate the potential of plant tolerance to environmental stress.

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“…Wang et al . () investigated the function of Ca 2+ in PCD of tobacco protoplasts and found that pretreatment of protoplasts with EGTA effectively retarded the PCD process. Buffering extracellular Ca 2+ with EGTA prevents [Ca 2+ ] cyt elevation, H 2 O 2 production, but does not prevent cell death in soybean cell culture (Zuppini et al .…”

Section: Introductionmentioning

confidence: 99%

“…Ethylene glycol bis (2-aminoethyl) tetraacetic acid (EGTA) is a Ca 2+ chelator, and is used to investigate the signaling role of Ca 2+ in cellular processes, such as the role of Ca 2+ as a cofactor in the activation of nucleases and potassium channel proteins in cells (Dominguez and Ward 2009;Almassy et al 2012). Wang et al (2010) investigated the function of Ca 2+ in PCD of tobacco protoplasts and found that pretreatment of protoplasts with EGTA effectively retarded the PCD process. Buffering extracellular Ca 2+ with EGTA prevents [Ca 2+ ]cyt elevation, H2O2 production, but does not prevent cell death in soybean cell culture (Zuppini et al 2004).…”

Section: Introductionmentioning

confidence: 99%

The Role of Ethylene and Calcium in Programmed Cell Death of Cold-Stored Cucumber Fruit

Chen

Zhao²,

Chen

et al. 2013

Journal of Food Biochemistry

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Programmed cell death (PCD) occurs during plant development and in response to various stimuli, including biotic and abiotic stresses. Characteristics of PCD were detected in cucumber fruit after 9 days of cold storage at 2C, including chromatin condensation and DNA fragmentation. PCD development was accompanied by ethylene emission and cytosolic free calcium ([Ca 2+ ]cyt) release. The role of ethylene production and [Ca 2+ ]cyt in chill-induced PCD were studied through the application of 1-methylcyclopropene (1-MCP; an ethylene perception inhibitor) and ethylene glycol bis (2-aminoethyl) tetraacetic acid (EGTA; a divalent cation chelator). Results indicated that hallmarks of PCD were identified in cucumber fruit suffering from chilling stress and diagnostic events of PCD were alleviated and/or delayed by 1-MCP or EGTA applications. Therefore, ethylene and calcium could play significant roles in the initiation and execution progress of coldinduced PCD. PRACTICAL APPLICATIONSCucumber fruit is chilling sensitive at temperatures below 10C. It has also been showed that exogenous ethylene could induce PCD features in cucumber fruits and [Ca 2+ ]cyt plays a key role in regulating apoptosis. However, information about roles of endogenous ethylene and calcium in the PCD process of cucumber under cold stress has been rarely reported. The data in this study provided a theoretical basis for understanding the mechanism of chilling injury in cucumber fruit and benefited exploration of strategies to prevent it.

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Role of calcium in nitric oxide-induced programmed cell death in tobacco protoplasts

Cited by 16 publications

References 38 publications

Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis

Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis

Transmembrane potential, an indicator in situ reporting cellular senescence and stress response in plant tissues

The Role of Ethylene and Calcium in Programmed Cell Death of Cold-Stored Cucumber Fruit

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