Role of cationic lipids for the formulation of lipoplexes

Abstract

Cationic lipids are widely used for their advantages over viral gene transfer as they are non-immunogenic and their production is easy. The formation of cationic liposomes to lipoplexes with the help of cationic lipids has been done. Cationic lipids are often used in combination with helper lipids such as DOPE or cholesterol for defining their structural properties. The mode of lipoplex formation has been described in this review. This review also focuses on the parameters that affects the physico-chemical properties of lipoplexes describing their use for the cationic lipid based on the gene therapy purposes. The current status and various prospects for the transfection efficacy of lipoplexes is also been described.

Magnetic Nanoparticles for Nucleic Acid Delivery: Magnetofection, Gene Therapy and Vaccines

“…Magnetolipoplexes are also widely used for in vivo magnetofection as they have the capability of saving the genetic material from adverse degradation during the transfection [86]. Cationic lipids, which behave as amphiphilic lipids, are preferable over viral gene vectors since they are non-immunogenic, can be easily prepared, form spontaneous complexes with the negatively charged DNA by electrostatic interactions and interact with the cell membrane inducing the endocytosis of the lipoplex and the following release of the genetic material into the cytoplasm.…”