Role of CBL‐Interacting Protein Kinases in Regulating Plant Stress Responses

Narayanan, Pavithran; Sanyal, Sibaji K.; Pandey, Girdhar K.

doi:10.1002/9781119541578.ch12

Cited by 4 publications

(1 citation statement)

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“…In wheat, bursts of ROS as early as 12 hpi are associated with the activation of defence signalling in incompatible interactions with Pst (Wang et al ., 2007 ). ROS are known to crosstalk with CBL‐CIPK signalling in plants to regulate signal transduction processes (Ma et al ., 2020 ; Narayanan et al ., 2020 ). In this study, knockout of TaCIPK14 resulted in lower expression levels of ROS scavenger‐related gene TaCAT2 during Pst infection.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous editing of three homoeologues of TaCIPK14 confers broad‐spectrum resistance to stripe rust in wheat

Tian

Zhao

et al. 2022

Plant Biotechnology Journal

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Summary Wheat stripe rust caused by the fungus Puccinia striiformis f. sp. tritici (Pst) is one of the most destructive wheat diseases resulting in significant losses to wheat production worldwide. The development of disease‐resistant varieties is the most economical and effective measure to control diseases. Altering the susceptibility genes that promote pathogen compatibility via CRISPR/Cas9‐mediated gene editing technology has become a new strategy for developing disease‐resistant wheat varieties. Calcineurin B‐like protein (CBL)‐interacting protein kinases (CIPKs) has been demonstrated to be involved in defence responses during plant‐pathogen interactions. However, whether wheat CIPK functions as susceptibility factor is still unclear. Here, we isolated a CIPK homoeologue gene TaCIPK14 from wheat. Knockdown of TaCIPK14 significantly increased wheat resistance to Pst, whereas overexpression of TaCIPK14 resulted in enhanced wheat susceptibility to Pst by decreasing different aspects of the defence response, including accumulation of ROS and expression of pathogenesis‐relative genes. We generated wheat Tacipk14 mutant plants by simultaneous modification of the three homoeologues of wheat TaCIPK14 via CRISPR/Cas9 technology. The Tacipk14 mutant lines expressed race‐nonspecific (RNS) broad‐spectrum resistance (BSR) to Pst. Moreover, no significant difference was found in agronomic yield traits between Tacipk14 mutant plants and Fielder control plants under greenhouse and field conditions. These results demonstrate that TaCIPK14 acts as an important susceptibility factor in wheat response to Pst, and knockout of TaCIPK14 represents a powerful strategy for generating new disease‐resistant wheat varieties with BSR to Pst.

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Section: Discussionmentioning

confidence: 99%