Role of CD4+ T lymphocytes and interleukin-5 in antigen-induced eosinophil recruitment into the site of cutaneous late-phase reaction in mice

Iwamoto, Itsuo; Tomoe, S; Tomioka, Hisao; Takatsu, Kiyoshi; Yoshida, Sho

doi:10.1002/jlb.52.5.572

Cited by 41 publications

(22 citation statements)

References 30 publications

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“…6). As previously reported in other allergic responses, such as airway inflammation and allergic cutaneous responses [22,25,26,27], we propose that in CS responses, IL-5 supports eosinophil production, survival and migration into the local tissue. We also propose that IL-5-dependent maintenance of B-1 cells and rapid early Ag-specific IgM production from B-1 cells are required for CS induction and elicitation.…”

Section: Discussionsupporting

confidence: 50%

“…Because IL-5 functions as a chemotactic factor for murine eosinophils [21], IL-5 signals could be required for eosinophils to migrate into the contact Ag-challenged skin. Iwamoto et al [22] have clearly demonstrated eosinophil recruitment into the site of cutaneous late-phase reaction that depends on IL-5. They showed that passive administration of neutralizing anti-mouse IL-5 antibodies suppressed cutaneous eosinophilia reaction induced by ovalbumin.…”

Section: Discussionmentioning

confidence: 99%

“…Although T cells, mast cells and eosinophils are known as a major source of IL-5-producing cells, our previous study suggests that maintenance of B-1 cells is supported by IL-5 that is produced by non-T/non-mast/non-eosinophil cells [11]. In cutaneous late-phase reactions, IL-5 produced by CD4+ T cells could promote eosinophil recruitment into the skin [22]. We are currently investigating which cell populations contribute to IL-5 production in CS responses.…”

Section: Discussionmentioning

confidence: 99%

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Interleukin 5 Plays an Essential Role in Elicitation of Contact Sensitivity through Dual Effects on Eosinophils and B-1 Cells

Itakura

Kikuchi

Kouro

et al. 2006

Int Arch Allergy Immunol

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Background: Elicitation of contact sensitivity (CS) depends on B-1-cell-derived antigen-specific immunoglobulin M (IgM) antibodies that recruit CS effector T cells into the local tissue, which is followed by infiltration of antigen-nonspecific mononuclear cells and polymorphonuclear cells, such as neutrophils and eosinophils. In this study, we investigated the role of interleukin (IL)-5, which has broad effects on both eosinophils and B-1 cells, in elicitation of CS. Methods: IL-5 receptor α-chain-deficient (IL-5Rα–/–) mice and IL-5Rα+/+ mice were contact sensitized with oxazolone hapten. Four days later, mice were challenged with the same hapten, and ear swelling responses were measured at 24 h after challenge. Eosinophil infiltration into the local tissue was determined by examination of skin histology and eosinophil peroxidase activity. To investigate the role of IL-5 in B-1 cell activation, the number of oxazolone-specific IgM-producing cells in the spleen was determined by enzyme-linked immunospot assay. Results: Ear swelling responses in IL-5Rα–/– mice were about half of those in IL-5Rα+/+ mice, and nearly no eosinophil infiltration was observed in IL-5Rα–/– mouse skin. Eosinophil peroxidase activity in the sensitized and challenged IL-5Rα–/– mice was about 11 times less than that in immunized IL-5Rα+/+ mice. Contact sensitization significantly increased in numbers of oxazolne-specific IgM-producing cells in IL-5Rα+/+ mouse spleen, but not in IL-5Rα–/– mouse spleen. Conclusion: We conclude that IL-5-dependent activation of eosinophils and B-1 cells is necessary for induction and elicitation of CS. These findings provide a new insight into complicated mechanisms of CS elicitation and suggest a novel role of IL-5 in the regulation of immune responses.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Interleukin 5 Plays an Essential Role in Elicitation of Contact Sensitivity through Dual Effects on Eosinophils and B-1 Cells

Itakura

Kikuchi

Kouro

et al. 2006

Int Arch Allergy Immunol

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“…According to some investigators, LTR is primarily an IgE-and mast-celldependent reaction (27,28), whereas according to others, LTR mainly corresponds to a CD4 T cell-mediated inflammatory response (29,30). Interestingly, CD4 T cells, particularly Th2 cells, and mast cells have been shown to produce common cytokines, so-called Th2 cytokines including IL-4 and IL-5, in vitro and in vivo (31,32).…”

Section: Discussionmentioning

confidence: 99%

The Combined Effect of Topical CX-659S, a Novel Diaminouracil Derivative, With Topical Corticosteroid on the Three Types of Allergic Responses in Mice or Guinea Pigs

2003

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Abstract. CX-659S ((S)-6-amino-5-(6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxamido)-3-methyl-1-phenyl-2,4(1H,3H)-pyrimidinedione), a newly discovered anti-inflammatory compound, exerts inhibitory effects against picryl chloride-, oxazolone-, and dinitrochlorobenzeneinduced acute contact hypersensitivity responses (CHRs) characterized by Th1-type reactions. Furthermore, this compound suppressed chronic CHRs characterized by Th2-type reactions, which is well known to mimic many, if not all, events occurring within the lesional skin of patients with atopic dermatitis (AD). The present study was conducted to determine the combined effect of topical CX-659S with topical corticosteroid on immediate type (ITR), late type (LTR), and delayed type hypersensitivity (DTHR) allergic reactions that are involved in AD. An ineffective dose of CX-659S (0.03 mg / ear) combined with betamethasone valerate (BV) significantly potentiated inhibitory activity of BV alone (0.1 m g / ear and 0.3 m g / ear) on both the ITR and the LTR in mice with the ovalbumin (OVA)-induced biphasic cutaneous reaction. Furthermore, the combined effect of CX-659S with BV was also observed on dinitrochlorobenzene (DNCB)-induced DTHR in guinea pigs. These results indicate that CX-659S has a combined effect with corticosteroids on every ITR, LTR, and DTHR. Proper treatment with corticosteroids for a safe and effective treatment of AD is needed. Thus, the combination therapy of topical CX-659S with topical corticosteroid would be one of the potential approaches for devising a proper treatment with corticosteroids.

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“…Those cells are generally believed to mediate the eosinophil accumulation observed during allergic reactions and parasitic infections through a mechanism dependent on IL-5 (Spry 1988, Frew et al 1989, Iwamoto et al 1992. To investigate the putative involvement of lymphocytes on the eosinophil accumulation triggered by LPS the animals were pretreated with cyclosporin A, an inhibitor of T-lymphocyte activation (Borel 1989).…”

Section: Cellular Source Of the Eosinophilotactic Activity Present Inmentioning

confidence: 99%

A role for lymphocytes and cytokines on the eosinophil migration induced by LPS

Castro‐Faria‐Neto¹,

Penido²,

Larangeira³

et al. 1997

Mem. Inst. Oswaldo Cruz

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In the present work we review the existing evidence for a LPS-induced cytokine-mediated eosinophil accumulation in a model of acute inflammation. Intrathoracic administration of LPS into rodents (mice, rats or guinea pigs) induces a significant increase in the number of eosinophils recovered from the pleural fluid 24 hr later. This phenomenon is preceded by a neutrophil influx and accompanied by lymphocyte and monocyte accumulation. The eosinophil accumulation induced by LPS is not affected by inhibitors of cyclo or lipoxygenase nor by PAF antagonists but can be blocked by dexamethasone or the protein synthesis inhibitor cycloheximide. Transfer of cell-free pleural wash from LPS injected rats (LPS-PW) to naive recipient animals induces a selective eosinophil accumulation within 24 hr. The eosinophilotactic activity present on the LPS-PW has a molecular weight ranging between 10 and 50 kDa and its effect is abolished by trypsin digestion of the pleural wash indicating the proteic nature of this activity. The production of the eosinophilotactic activity depends on the interaction between macrophages and T-lymphocytes and its effect can not be blocked by anti-IL-5 monoclonal antibodies. Accumulated evidence suggest that the eosinophil accumulation induced by LPS is a consequence of a eosinophilotactic cytokine produced through macrophage and T-cell interactions in the site of a LPSinduced inflammatory reaction.

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Role of CD4+ T lymphocytes and interleukin-5 in antigen-induced eosinophil recruitment into the site of cutaneous late-phase reaction in mice

Cited by 41 publications

References 30 publications

Interleukin 5 Plays an Essential Role in Elicitation of Contact Sensitivity through Dual Effects on Eosinophils and B-1 Cells

Interleukin 5 Plays an Essential Role in Elicitation of Contact Sensitivity through Dual Effects on Eosinophils and B-1 Cells

The Combined Effect of Topical CX-659S, a Novel Diaminouracil Derivative, With Topical Corticosteroid on the Three Types of Allergic Responses in Mice or Guinea Pigs

A role for lymphocytes and cytokines on the eosinophil migration induced by LPS

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