Role of cell-associated<i>N</i>-acetyl-d-glucosamine specific haemagglutinin in the adhesion of<i>Vibrio cholerae</i>O1 to rabbit intestinal epithelial cells in vitro

Sasmal, D.; Guhathakurta, B.; Bhattacharya, Subhajit; Pal, Chiranjib; Datta, A.

doi:10.1111/j.1574-695x.1996.tb00222.x

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…Bacterial ligands mediating adhesion to chitin by a specific binding to Nacetylglucosamine (GlcNAc) have been shown to be present in V. cholerae classical strains. A cellassociated GlcNAc specific hemagglutinin (HA) was purified from V. cholerae by affinity chromatography on a chitin column, followed by BioGel filtration (16). Chitin-binding proteins (CBPs) associated with the cell wall have been described by Tarsi and Pruzzo (17) and Zampini et al (18), who extended previous studies on Vibrio harveyi (19) and Vibrio alginolyticus (20), and identified two surface proteins (36 kDa and 53 kDa) involved in adhesion of V. cholerae classical strains to chitin beads and copepods.…”

Section: Introductionmentioning

confidence: 99%

Adhesins acquired in the aquatic environment andVibrio choleraecolonization of intestinal cells

Vezzulli

Repetto

Pezzati

et al. 2008

Microbial Ecology in Health & Disease

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Recent results for Vibrio cholerae interactions with bivalves and chitin-containing substrates are reviewed. Chitin, composed of b-1,4-linked N-acetylglucosamine residues, is one of the most abundant biopolymers in nature and the most abundant in the marine environment. V. cholerae connection to chitin is a well known phenomenon and one of the best documented examples of a successful bacteriaÁsubstrate interaction, affecting both the lifestyle of the microorganisms and natural system functioning. In sea water, vibrios can be entrapped by filter-feeding invertebrates that sieve suspended food particles from the aquatic environment. Persistence of bacteria in bivalves depends on different factors including their sensitivity to the bactericidal activity of hemolymph. Both V. cholerae survival inside bivalves and chitin colonization depend on cell surface properties, with contribution of different ligands (e.g. chitin-binding proteins, MSHA). The role of these ligands in interactions with human surfaces is discussed and evidence is presented supporting (i) the link between vibrio persistence in the environment (both inside bivalves and adhering to chitin-containing surfaces) and infection of the human host, and (ii) the hypothesis that virulence mechanisms of bacteria that have environmental reservoirs represent a consequence of adaptive mechanisms to the environment.

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Section: Introductionmentioning

confidence: 99%

Adhesins acquired in the aquatic environment andVibrio choleraecolonization of intestinal cells

Vezzulli

Repetto

Pezzati

et al. 2008

Microbial Ecology in Health & Disease

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Global impact of Vibrio cholerae interactions with chitin

Pruzzo

Vezzulli

Colwell

2008

Environmental Microbiology

243

201

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SummaryThe interaction of Vibrio cholerae with chitin exemplifies for microbial ecology a successful bacteriasubstrate interaction with complex and significant influence on the lifestyle of the bacterium. Chitin is one of the most abundant polymers on earth and possibly the most abundant in the aquatic environment, where its association with V. cholerae has provided the microorganism with a number of advantages, including food availability, adaptation to environmental nutrient gradients, tolerance to stress and protection from predators. Emergent properties of V. cholerae-chitin interactions occur at multiple hierarchical levels in the environment and include cell metabolic and physiological responses e.g. chemotaxis, cell multiplication, induction of competence, biofilm formation, commensal and symbiotic relationship with higher organisms, cycling of nutrients, and pathogenicity for humans and aquatic animals. As factors mediating virulence of V. cholerae for humans and aquatic animals derive from mechanisms of adaptation to its environment, at different levels of hierarchical scale, V. cholerae interactions with chitin represent a useful model for examination of the role of primary habitat selection in the development of traits that have been identified as virulence factors in human disease.

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Host, pathogen and environment: a bacterial gbpA gene expression study in response to magnesium environment and presence of prawn carapace and commercial chitin

Tiruvayipati

Bhassu

2016

Gut Pathog

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BackgroundVibrio parahaemolyticus is a Gram-negative halophilic bacterium which is found largely in estuarine and coastal waters. The bacteria has been a main focus in gastro-intestinal infections caused primarily due to the consumption of contaminated seafood. It was shown to survive in magnesium concentrations as high as 300 mM which are toxic to various other micro-organisms. Several genes of V. parahaemolyticus were studied, among which gbpA (N-acetyl glucosamine binding protein) was reported in Vibrio cholerae.MethodsThe current study investigates the V. parahaemolyticus gbpA gene expression at different concentrations of magnesium sulfate heptahydrate (MgSO4·7H2O, chosen as the magnesium environment), in the presence of the host’s (prawn) carapace and the mimicked carapace [commercial chitin flakes (Sigma)]. The concentrations of MgSO4·7H2O utilized were approximately 0, 1, 75, 137, 225 and 300 mM. These were selected based upon the survival conditions required by prawn and bacteria, respectively. 0.05 gm/3 ml of carapace (by dry weight) and commercial chitin flakes were used in the experiments. Bacterial count was performed for the biological triplicates for the 3 experimental setups. The genome of Vibrio parahaemolyticus PCV08-7 (VPPCV08-7) was used as a reference, based on whose translated gbpA gene the probable protein-chemical interactions were determined on the STITCH database.ResultsThe GbpA protein was shown to interact with chitin on the STITCH database. In our experiments, the gbpA showed lower gene expression levels at different MgSO4·7H2O concentrations in the presence of chitin and carapace, than with the presence of only MgSO4•7H2O. In addition, the bacterial count for various concentrations of magnesium used, revealed a distinct decrease in bacterial count within and among each of the three experimental setups.ConclusionIn the presence of only magnesium, an increase in the gbpA expression with neither chitin nor carapace and vice versa supported by the results from the bacterial counts could help further studies to prove that the moulting phase of prawns may trigger increased expression of the V. parahaemolyticus gbpA gene.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-016-0105-5) contains supplementary material, which is available to authorized users.

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Role of cell-associatedN-acetyl-d-glucosamine specific haemagglutinin in the adhesion ofVibrio choleraeO1 to rabbit intestinal epithelial cells in vitro

Cited by 3 publications

References 13 publications

Adhesins acquired in the aquatic environment andVibrio choleraecolonization of intestinal cells

Adhesins acquired in the aquatic environment andVibrio choleraecolonization of intestinal cells

Global impact of Vibrio cholerae interactions with chitin

Host, pathogen and environment: a bacterial gbpA gene expression study in response to magnesium environment and presence of prawn carapace and commercial chitin

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