Role of cell proliferation and vascularity in malignant transformation of potentially malignant disorders

Sheelam, Suchita; Reddy, Shyam Prasad; Kulkarni, Pavan G; Nandan, S R K; Muddana, Keerthi; Raj, G Shyam

doi:10.4103/jomfp.jomfp_182_17

Cited by 9 publications

(19 citation statements)

References 20 publications

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“…As for the pathogenesis of OSCC, smoking is considered as a primary factor and excessive alcohol drinking, betel nut chewing and human papillomavirus infection can also cause OSCC [7,8]. According to previous studies, high proliferation and EMT are also urgent threats in treating OSCC [9,10]. Although OSCC is the sixth most common cancer occurring in humans, its underlying mechanism is still unclear.…”

Section: Introductionmentioning

confidence: 99%

LncRNA NEAT1 mediates progression of oral squamous cell carcinoma via VEGF-A and Notch signaling pathway

Zhu

Shu

et al. 2020

World J Surg Onc

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Background lncRNAs and VEGF have been shown to have close connections with oral squamous cell carcinoma (OSCC). We explored the interaction between lncRNA NEAT1 and VEGF-A in OSCC. Methods RT-qPCR was implemented to measure levels of lncRNA NEAT1 and VEGF-A in OSCC cell lines and normal cell lines. Cell functions then were checked after regulating the expressions of lncRNA NEAT1 and VEGF-A separately. Cell viabilities were examined with CCK-8 and apoptosis rate was checked with flow cytometry. Meanwhile, EMT-related genes E-cadherin, N-cadherin, Vimentin, and Snail and Notch signaling genes Notch1, Notch2, and Jagged were evaluated by RT-qPCR. IMR-1 was applied for impeding Notch signaling pathway. Later, cell viabilities, apoptosis, and EMT were assessed. Results Expressions of lncRNA NEAT1 and VEGF-A were both increased significantly in OSCC cell lines especially in TSCC1 cell line. Suppression of lncNRA NEAT1 was associated with lower cell viabilities and EMT and higher apoptosis rate in the TSCC1 cell line. Meanwhile, knockdown of VEGF-A significantly repressed cell viabilities and EMT in the TSCC1 cell line. Magnifying functions of inhibited lncRNA NEAT1 Notch signaling pathway was obviously activated with overexpressions of lncRNA NEAT1 and VEGF-A. Adding IMR-1 significantly downregulated cell viabilities and EMT and sharply increased apoptosis in the context of lncRNA NEAT1 and VEGF-A overexpression. Conclusion LncRNA NEAT1 may upregulate proliferation and EMT and repress apoptosis through activating VEGF-A and Notch signaling pathway in vitro, suggesting an underlying regulatory factor in OSCC. Nevertheless, further research is necessary to gain a greater understanding of lncRNA NEAT1 and connections with VEGF-A in vivo and in clinical study.

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Section: Introductionmentioning

confidence: 99%

LncRNA NEAT1 mediates progression of oral squamous cell carcinoma via VEGF-A and Notch signaling pathway

Zhu

Shu

et al. 2020

World J Surg Onc

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“…Data regarding the quantitative assessment of angiogenesis from 21 studies 13–16,18,19,21–25,29–37,40 has been elaborated in Table S3. Thirteen 13,14,16,19,21,23,31–36,40 assessed the angiogenic status by calculating the microvessel density (MVD).…”

Section: Resultsmentioning

confidence: 99%

An exotic pathogenetic mechanism of angiogenesis in oral lichen planus—A systematic review

Keerthika

Kamboj

Girdhar

et al. 2023

J Oral Pathology Medicine

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ObjectiveAngiogenesis plays a vital role at the molecular level in various inflammatory lesions, that lead to their chronicity. Oral lichen planus is an immune‐mediated chronic inflammatory disorder. The angiogenetic role and exact mechanisms in oral lichen planus are still unclear due to a dearth of studies. Its clinical significance with angiogenesis also requires further elucidation necessitating a thorough review of the studies that have been conducted so far. The present review was designed to identify the dependence of oral lichen planus progression on angiogenesis which could aid in devising metronomic treatments required to halt the progression of this disease.Materials and MethodsA thorough search was made using MEDLINE by PubMed, Scopus, Google scholar, Cochrane library, and EMBASE databases. Original research articles, that immunohistochemically evaluated angiogenesis in oral lichen planus were included for review. Risk of bias was analysed for each study using Modified Newcastle‐Ottawa scale and Review Manager 5.4 was used to output its result.ResultsTwenty‐nine published articles were included for data synthesis. The most commonly employed antibody was CD34, however, upregulated VEGF expression was the principal while ICAM‐1, VCAM‐1, and PECAM‐1 were critical angiogenic factors to mediate angiogenesis in oral lichen planus.ConclusionThe current evidence supports that angiogenesis, a fundamental pathogenetic mechanism of oral lichen planus, leads to its persistence and chronicity. However, studies with a larger sample size, standard evaluation criteria, different subtypes, and adequate follow‐up are warranted.

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“…This can be explained as diosmin at dose 100mg/kg to DMBA painted hamsters acted as a perfect scavenger against the ROS generated by DMBA as it is tremendously reactive as electron donor limiting the damage caused by ROS in cells (30) , its effective antioxidant effect helped in tumor reduction which could be seen by the increased TBARS in the plasma and erythrocytes, while it is decreased in the buccal tissues due to the ability of that dose to restore glutathione reductase which becomes able to scavenge free residual radicals, Vitamin C and Vitamin E levels, both vitamins have various biological importance influencing the prevention of the damage of the gene and lipid through their modification of any carcinogenic metabolic stimulator; so diosmin at that dose was able to normalize the toxicity induced by DMBA suggesting that diosmin has anti lipid peroxidative property and that it balances the harmful oxidants and protective antioxidants as it can adjust the antioxidant enzymes. It also decreases the levels of JAK-1/STAT-3 phosphorylated forms and so its translocation to the nucleus resulting in inhibiting JAK-1/STAT-3 signal decreasing the proliferation of cells (23)(24)(25)(26)(27)(28)(29)(30)(31) .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Dose Dependent Chemopreventive Effect of Diosmin on Induced Oral Squamous Cell Carcinoma

et al. 2022

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Purpose: This study aimed to evaluate the chemoprevention efficacy of diosmin on experimentally induced hamster buccal pouch (HBP) squamous cell carcinoma utilizing Ki 67 as a proliferative marker. Material and Methods: Seventy Syrian male hamsters were used as experimental animals, these hamsters were divided into five groups, Group I (negative control): 5 hamsters were left untreated. Group II (DMBA treated group): 20 hamsters were divided equally into two subgroups, painted with 0.5% DMBA where subgroup IIa: for 8 weeks while subgroup IIb: for 14 weeks. Group III: 5 hamsters were orally administrated diosmin 150 mg/kg for 14 weeks. Group IV: 20 hamsters were divided equally into two subgroups, treated with diosmin 100mg/kg and DMBA where subgroup IVa: for 8 weeks while subgroup IVb: for 14 weeks. Group V: 20 hamsters were divided equally into two subgroups, treated with diosmin 150mg/ kg and DMBA where subgroup Va: for 8 weeks while subgroup Vb: for 14 weeks.Results: Comparing all groups and subgroups revealed that the mean values have been arranged with the following descending pattern subgroup IIa, subgroup Va, subgroup IIb, subgroup Vb, subgroup IVb, subgroup IVa, group III and group I. ANOVA test revealed a statistically significant difference between groups. Conclusion: Diosmin 100mg/kg is considered a promising chemopreventive agent in preventing induced HBP squamous cell carcinoma.

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Role of cell proliferation and vascularity in malignant transformation of potentially malignant disorders

Cited by 9 publications

References 20 publications

LncRNA NEAT1 mediates progression of oral squamous cell carcinoma via VEGF-A and Notch signaling pathway

LncRNA NEAT1 mediates progression of oral squamous cell carcinoma via VEGF-A and Notch signaling pathway

An exotic pathogenetic mechanism of angiogenesis in oral lichen planus—A systematic review

Evaluation of Dose Dependent Chemopreventive Effect of Diosmin on Induced Oral Squamous Cell Carcinoma

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