Role of cellular actin in human parainfluenza virus type 3 genome transcription.

De, Bishnu P.; Burdsall, Andrea L.; Banerjee, Amiya K.

doi:10.1016/s0021-9258(18)53375-5

Cited by 56 publications

(4 citation statements)

References 58 publications

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“…Cytoskeletal proteins have also been implicated in the activation of transcription of paramyxoviruses (measles virus, Sendai virus, respiratory syncytial virus) and rhabdoviruses (VSV) (58)(59)(60). For example, in paramyxoviruses such as human parainfluenza virus type 3, polymerized actin has been shown to be necessary for the activation of transcription from the ribonucleoprotein complex in vitro and in vivo (61)(62)(63). It has been suggested that upon interaction with actin, the paramyxovirus ribonucleoprotein complex undergoes a conformational alteration from a loosely coiled to a moderately condensed form, which facilitates transcription (62).…”

Section: Discussionmentioning

confidence: 99%

“…For example, in paramyxoviruses such as human parainfluenza virus type 3, polymerized actin has been shown to be necessary for the activation of transcription from the ribonucleoprotein complex in vitro and in vivo (61)(62)(63). It has been suggested that upon interaction with actin, the paramyxovirus ribonucleoprotein complex undergoes a conformational alteration from a loosely coiled to a moderately condensed form, which facilitates transcription (62). It is possible that activation of HIV-1 reverse transcription also requires a conformational change in the reverse transcription complex, and that this is mediated through interaction with the actin microfilaments.…”

Section: Discussionmentioning

confidence: 99%

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Establishment of a Functional Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcription Complex Involves the Cytoskeleton

Bukrinskaya

Břicháček

Mann

et al. 1998

The Journal of Experimental Medicine

248

223

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After interaction of human immunodeficiency virus type 1 (HIV-1) virions with cell surface receptors, a series of poorly characterized events results in establishment of a viral reverse transcription complex in the host cell cytoplasm. This process is coordinated in such a way that reverse transcription is initiated shortly after formation of the viral reverse transcription complex. However, the mechanism through which virus entry and initiation of reverse transcription are coordinated and how these events are compartmentalized in the infected cell are not known. In this study, we demonstrate that viral reverse transcription complexes associate rapidly with the host cell cytoskeleton during HIV-1 infection and that reverse transcription occurs almost entirely in the cytoskeletal compartment. Interruption of actin polymerization before virus infection reduced association of viral reverse transcription complexes with the cytoskeleton. In addition, efficient reverse transcription was dependent on intact actin microfilaments. The localization of reverse transcription to actin microfilaments was mediated by the interaction of a reverse transcription complex component (gag MA) with actin but not vimentin (intermediate filaments) or tubulin (microtubules). In addition, fusion, but not endocytosis-mediated HIV-1 infectivity, was impaired when actin depolymerizing agents were added to target cells before infection but not when added after infection. These results point to a previously unsuspected role for the host cell cytoskeleton in HIV-1 entry and suggest that components of the cytoskeleton promote establishment of the reverse transcription complex in the host cell and also the process of reverse transcription within this complex.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Establishment of a Functional Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcription Complex Involves the Cytoskeleton

Bukrinskaya

Břicháček

Mann

et al. 1998

The Journal of Experimental Medicine

248

223

View full text Add to dashboard Cite

show abstract

“…As regards virus replication in host cells, many studies have demonstrated that various viruses interact with the actin cytoskeleton at different stages of their life cycles. For instance, De et al [3] reported interactions between actin and the ribonucleoprotein complex of human parainfluenza virus type 3, demonstrating that both polymeric and monomeric forms of actin bind strongly to the ribonucleoproteins, possibly playing a role in mRNA synthesis. Gupta et al [7] further reported that the viral nucleocapsids of human parainfluenza virus type 3 are specifically localized on actin microfilaments, and the treatment of cells with the actin-depolymerizing agent cytochalasin D (C-D), a reagent that disrupts the equilibrium between monomeric and polymeric actin (see below), resulted in the inhibition of viral RNA synthesis and ribonucleoprotein accumulation.…”

mentioning

confidence: 99%

Role of Actin Microfilaments in Canine Distemper Virus Replication in Vero Cells

Katayama

Hori

Sato

et al. 2004

The Journal of Veterinary Medical Science

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Several studies have indicated that viruses require a specific cytoskeletal structure for replication in host cells. In this study, we examined the role of actin fiber in the replication of canine distemper virus (CDV), belonging to the Morbillivirus genus of the family Paramyxoviridae. For this purpose, we used two actin depolymerizing agents, cytochalasin-D (C-D) and mycalolide-B (ML-B). In Vero cells, C-D disrupted actin fibers distributed in the cytosol, but peripheral actin fibers remained intact. On the other hand, ML-B completely disrupted the actin fibers distributed in both areas. Treatment of Vero cells with C-D or ML-B inhibited the replication of CDV. Double staining of CDV-infected Vero cells with antibody to N-protein and rhodamine-phalloidin revealed the presence of N-protein in mid-cytoplasm. However, the N-protein was specifically localized at the submembrane region in the presence of C-D, whereas it was clustered in the presence of ML-B. Viral mRNA levels of N- and H-proteins were rather increased by treatment with C-D or ML-B. The treatment with ML-B strongly inhibited N-protein expression, whereas C-D only slightly inhibited N-protein expression. These results suggest that actin microfilaments distributed in the cytoplasm and on the membrane region in host cells may have a different role in the process of CDV replication.

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“…Daneben wurden auch einige Wirts-Proteine identifiziert, die für die virale RNA-Synthese notwendig sind. Dazu gehören Tubulin, Aktin, Profilin (ein Aktin-bindendes Protein), Translations-Elongationsfaktor 1, Dynein und verschiedene Hitzeschock-Proteine (Moyer et al 1990, De et al 1993, Burke et al 2000, Das et al 1998, Qanungo et al 2004, Poisson et al 2001, Zhang et al 2002a Hyphen umhüllt und differenzieren so zunächst zu Vor-Fruchtkörpern (Protoperithezien), welche nach ca. sieben Tagen zu den komplex strukturierten Fruchtkörpern (Perithezien) ausgereift sind.…”

Section: Kürzlich Wurde Eine Interaktion Von Striatin-proteinen Mit D...unclassified

Untersuchung der Fruchtkörperentwicklung bei dem Hyphenpilz Sordaria macrospora

Bernhards¹

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Die Familie der Striatin-Proteine aus Eukaryoten 2.1 Die vielfältigen Funktionen von Striatin-Proteinen 2.2 Interaktionspartner von Striatin-Proteinen und die Signalwege in denen sie wirken 2.2.1 Phocein/Mob3 -ein Protein aus der Mob-Familie 2.2.2 Die Protein-Phosphatase 2A (PP2A) 2.2.3 ER -Östrogen-vermittelte nicht-genomische und genomische Signalwege 2.2.4 Die Rolle von Striatin-Proteinen bei der Zell-Adhäsion 2.2.5 Die Rolle von Striatin-Proteinen bei der Virus-Assemblierung Das S. macrospora Protein Sm9375 -Beteiligung eines GPI-geankerten Proteins am SmMOB3/PRO11-Komplex 4 Reguliert der PRO11/SmMOB3-Komplex während Hyphenfusion und Fruchtkörperentwicklung die Endocytose und den Vesikel-Transport? 5 Zusammenfassung der vielfältigen Funktionen von S. macrospora PRO11-Proteinkomplexen bei zellulären Differenzierungsprozessen V ZUSAMMENFASSUNG

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Role of cellular actin in human parainfluenza virus type 3 genome transcription.

Cited by 56 publications

References 58 publications

Establishment of a Functional Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcription Complex Involves the Cytoskeleton

Establishment of a Functional Human Immunodeficiency Virus Type 1 (HIV-1) Reverse Transcription Complex Involves the Cytoskeleton

Role of Actin Microfilaments in Canine Distemper Virus Replication in Vero Cells

Untersuchung der Fruchtkörperentwicklung bei dem Hyphenpilz Sordaria macrospora

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