Role of Cellular Senescence in Lifestyle-Related Disease

Minamino, Tohru

doi:10.1253/circj.cj-10-0916

Cited by 18 publications

(11 citation statements)

References 71 publications

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“…26,27) P16 and/or p21 deficiency partially prevented the age-induced decline in cell proliferation and tissue function. 28) In this study, we found that Colla corii asini could remarkably decrease p16 and p21 expression. The results suggested that Colla corii asini could modulate age-related gene expression.…”

Section: ) D-galmentioning

confidence: 50%

Effect of Colla corii asini (E’jiao) on D-Galactose Induced Aging Mice

Wang

Liu

Cao

et al. 2012

Biological & Pharmaceutical Bulletin

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Colla corii asini (E'jiao), donkey-hide gelatin prepared by stewing and concentrating from Equus asinus L. donkey hide, is a traditional Chinese medicine preparation widely used in clinical hematic antanemic therapy in China. The aim of the present study was to investigate potential anti-aging effect of Colla corii asini and explore related mechanisms in D-galactose (gal) induced aging model mice. The mice were artificially induced aging by subcutaneously injection with D-gal at the dose of 100 mg/kg·d for 8 weeks. Colla corii asini was simultaneously treated to them once daily by intragastric gavage. Appetite, mental condition, body weight, and organ index were observed. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), as well as levels of malondialdehyde (MDA) in serum, brain, and liver were determined by according assay kits. Western blotting analysis was used to detect p16 and p21 expression. Results indicated that Colla corii asini could improve appetite, mental condition, body weight, and organ condition of model mice, improve SOD, CAT, and GSH-Px activities, reduce MDA levels, and modulate agerelated genes expression in D-gal induced mice. Therefore, Colla corii asini may have effect to suppress the aging process through enhancing antioxidant activity, scavenging free radicals, and modulating aging-related gene expression.Key words Colla corii asini (E' jiao); anti-aging; D-galactose; antioxidant; aging-related gene Aging is a natural phenomenon, and it is always associated with diverse chronic diseases, including cancer, Parkinson's and cardiovascular diseases, etc.1,2) Anti-aging has already become a major public issue with the increasing elderly population in the world. The free radical theory of aging was conceived by Harman in 1956. 3) Abundant evidences suggest that oxidative stress plays a central role in the process of biological aging.4) Oxygen-derived free radicals exert detrimental effects on human, including peroxidation of membrane lipids, enzyme inactivation, DNA fragmentation, and activation of apoptosis.5) In addition, supplementation with antioxidants has been reported to be beneficial with respect to slowing this aging process.D-Galactose (D-gal) has been used to induce oxidative stress in vivo to mimic the natural aging in mice. D-Gal can be metabolized at normal concentration, but when at high levels, it can be converted into galactitol under the catalysis of gal oxidase, resulting in the generation of superoxide anions and oxygen-derived free radicals.6) As one of the antioxidant defense systems, a group of enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), act as superoxide anion and H 2 O 2 scavengers to prevent reactive oxygen species (ROS)-induced damage, which may cause the changes of some biomarkers.7) Malondialdehyde (MDA) is a major biomarker that appears during the final stages of lipid peroxidation initiated by excessive ROS. An increase in the hepatic MDA concentration suggests th...

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Section: ) D-galmentioning

confidence: 50%

Effect of Colla corii asini (E’jiao) on D-Galactose Induced Aging Mice

Wang

Liu

Cao

et al. 2012

Biological & Pharmaceutical Bulletin

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“…It is of note that Vasa et al [17] used the same multiple passage of HUVECs s used in the present study. There is, however, a significant disparity in the rate of senescence in the vascular system depending on local parameters of hemodynamic stress and cellular turnover [20], [21].…”

Section: Discussionmentioning

confidence: 99%

Potential Involvement of LOX-1 in Functional Consequences of Endothelial Senescence

2011

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Numerous studies have described the process of senescence associated with accumulation of oxidative damage, mutations and decline in proliferative potential. Although the changes observed in senescent cells are likely to result in significant phenotypic alterations, the studies on consequences of endothelial senescence, especially in relation to aging-associated diseases, are scarce. We have analyzed effects of senescence on the functions of endothelial cells relevant to the development of atherosclerosis including angiogenesis, adhesion, apoptosis and inflammation. In the course of progressing through the passages, human umbilical vein endothelial cells (HUVECs) displayed significant increase in size (+36% passage 12 vs. passage 4 , p<0.001) and reduction in both basal and VEGF-stimulated tube formation. The analysis of a scavenger receptor LOX-1, a key molecule implicated in atherogenesis, revealed a significant decline of its message (mRNA) and protein content in senescent endothelial cells (−33%) and in aortas of 50 wk (vs. 5 wk) old mice (all p<0.01). These effects were accompanied by a marked reduction of the basal expression of VCAM-1 and ICAM-1. Compared to early cultures, late passage HUVECs also exhibited nuclear translocation of NF-κB (p65) and reciprocal shifts in BAX and BCL2 protein content resulting in almost 2-fold increase in BAX/BCL2 ratio and 3-fold increase in apoptotic response to TNFα exposure (p<0.04). These changes in senescent endothelial cells are suggestive of aberrant responses to physiological stimuli resulting in a less permissive environment for tissue remodeling and progression of diseases requiring angiogenesis and cell adhesion in elderly, possibly, mediated by LOX-1.

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“…This might include measures such as telomere length change over time, lens opacities, especially in younger individuals, modifications of insulin-like growth factors and its binding proteins, mitochondrial functional changes, immune marker characteristics, and specific genetic markers of aging [127,137–141]. …”

Section: Risk Factors For Ad and Normal Agingmentioning

confidence: 99%

Dementia and Alzheimer's disease: A new direction. The 2010 Jay L. Foster Memorial Lecture

Kuller

López

2011

Alzheimer's & Dementia

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Background The modern era of Alzheimer’s disease (AD) research began in the early 1980s with the establishment of AD research centers and expanded research programs at the National Institute on Aging. Methods Over the past 30 years, there has been success in defining criteria for AD and dementia, association of important genetic disorders related to premature dementia in families, the association of apolipoprotein-E4, and measurement of incidence and prevalence and selected risk factors. However, prevention and treatment have been elusive. Results The development of new technologies, especially magnetic resonance imaging, positron emission tomography to measure amyloid in vivo in the brain and glucose metabolism, cerebrospinal fluid examination, better genetic markers, large-scale longitudinal epidemiology studies, and preventive clinical trials has rapidly begun a new era of research that offers opportunities to better understand etiology, that is, determinants of amyloid biology in the brain, neurofibrillary tangles, synaptic loss, and dementia. Conclusions There are three major hypotheses related to dementia: amyloid deposition and secondary synaptic loss as a unique disease, vascular injury, and “aging.” New research must be hypothesis-driven and lead to testable approaches for treatment and prevention.

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Role of Cellular Senescence in Lifestyle-Related Disease

Cited by 18 publications

References 71 publications

Effect of <i>Colla corii asini</i> (<i>E’jiao</i>) on <small>D</small>-Galactose Induced Aging Mice

Effect of <i>Colla corii asini</i> (<i>E’jiao</i>) on <small>D</small>-Galactose Induced Aging Mice

Potential Involvement of LOX-1 in Functional Consequences of Endothelial Senescence

Dementia and Alzheimer's disease: A new direction. The 2010 Jay L. Foster Memorial Lecture

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