Role of Changes in σ<sup>70</sup>‐Driven Transcription in Adaptation of<i>E. coli</i>to Conditions of Stress or Starvation

Sharma, Uday

doi:10.1002/9781119004813.ch5

Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria 2016

DOI: 10.1002/9781119004813.ch5

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Role of Changes in σ⁷⁰‐Driven Transcription in Adaptation ofE. colito Conditions of Stress or Starvation

Uday Sharma¹

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2018

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Biofuel production with a stress-resistant and growth phase-independent promoter: mechanism revealed by in vitro transcription assays

Huo

Guo

2018

Appl Microbiol Biotechnol

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The carbon and energy needed for bioconversion processes face trade-offs between cell reproduction and chemical synthesis. In most processes, microbial cells containing overexpressed pathway enzymes were accumulated in exponential phase before the productions of value-added chemicals dominate the carbon and energy fluxes in stationary phase. The pathway enzymes need to be continuously supplied to compensate their degradation, but the promoters driving their overexpressions are downregulated under stationary phase or stressed conditions. In this study, the gadA promoter enabled stress-resistant and growth phase-independent isobutanol production up to 10-28 g l. To investigate the activation mechanism of gadA promoter and its potential in metabolic engineering, an in vitro transcription system was established. Results showed that gadA promoter could be transcribed efficiently under environments that inhibit the transcription of ribosomal promoters, while under moderate to rapid growth conditions, the large majority of new cellular transcripts are ribosomal. This differential transcription relies on the accumulation of environmental glutamate and/or the loss of supercoiling. These results implied that the gadA promoter could be functional or even dominate the cellular transcription under an exceedingly wide range of physiological conditions. Therefore, the gadA promoter is a novel candidate for driving pathway enzyme overexpressions under not only exponential phase but also stationary phase and stressed conditions, which is important for achieving efficient biofuel production.

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Biofuel production with a stress-resistant and growth phase-independent promoter: mechanism revealed by in vitro transcription assays

Huo

Guo

2018

Appl Microbiol Biotechnol

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Role of Changes in σ⁷⁰‐Driven Transcription in Adaptation ofE. colito Conditions of Stress or Starvation

Cited by 1 publication

References 92 publications

Biofuel production with a stress-resistant and growth phase-independent promoter: mechanism revealed by in vitro transcription assays

Biofuel production with a stress-resistant and growth phase-independent promoter: mechanism revealed by in vitro transcription assays

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Role of Changes in σ70‐Driven Transcription in Adaptation ofE. colito Conditions of Stress or Starvation

Cited by 1 publication

References 92 publications

Biofuel production with a stress-resistant and growth phase-independent promoter: mechanism revealed by in vitro transcription assays

Biofuel production with a stress-resistant and growth phase-independent promoter: mechanism revealed by in vitro transcription assays

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Role of Changes in σ⁷⁰‐Driven Transcription in Adaptation ofE. colito Conditions of Stress or Starvation