Role of chemical crosslinking in material-driven assembly of fibronectin (nano)networks: 2D surfaces and 3D scaffolds

Serra, Roser Sabater i; León-Boigues, Laia; Sánchez-Laosa, Antonio; Gomez-Estrada, Luis; Ribelles, J.L. Gómez; Salmerón-Sánchez, Manuel; Ferrer, Gloria Gallego

doi:10.1016/j.colsurfb.2016.08.044

Cited by 13 publications

(10 citation statements)

References 60 publications

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“…Nonetheless, hydrogels with up to 2 wt% ethylene glycol dimethacrylate could still achieve the same results as those previously seen on 2D surfaces. [128] It has also been demonstrated that plasma polymerized PEA, a production technique with more translational potential, can produce similar results. [93] This approach was recently tested to coat polyimide tube implants, which provide a bioinert mechanical support for long bone fracture healing.…”

Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 93%

“…This has been the strategy explored in a series of works using poly(ethyl acrylate) (PEA) as a base polymer. [93,[127][128][129] Mechanistically, FN adsorption onto PEA promotes its spontaneous fibrillar reorganization, a process that has been termed "material-driven FN fibrillogenesis," while adsorption on other polymers results in retention of a globular conformation. This fibrillary conformation leads to a rearrangement of FN chains and simultaneous exposure of affinity-binding regions for both integrins and GFs.…”

Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 99%

“…Remarkably, this behavior of FN on PEA surfaces is highly specific, even when compared to other closely related control polymers such as poly(methyl acrylate). [93,[127][128][129] These FN conformational rearrangements have been shown to be crucial for enhancing GF presentation, attaining impressive results in vitro when used along with BMP-2 for driving osteogenesis of MSCs. Moreover, the same combination was able to drive full regeneration of a critical sized defect in vivo at concentrations over 300-fold lower than clinical standards.…”

Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 99%

“…Remarkably, this behavior of FN on PEA surfaces is highly specific, even when compared to other closely related control polymers such as poly(methyl acrylate). [ 93,127–129 ]…”

Section: Solid‐phase Presentationmentioning

confidence: 99%

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Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are proteins secreted by cells that regulate a variety of biological processes. Although they have long been proposed as potent therapeutic agents, their administration in a soluble form has proven costly and ineffective due to their short half-lives in biological environments. Biomaterial-based approaches are increasingly sought as alternatives to improve the efficacy or, ideally, replace the need for exogenous administration of GFs in regenerative medicine strategies. The means by which these systems evolve from biomaterials for conventional controlled release of GFs to the recent extracellular matrix (ECM)-inspired approaches for sequestering these labile molecules and regulating their spatiotemporal activity and presentation are reviewed. Focus is placed on biomaterials functionalized either with ECM components, which show promiscuous GF binding, or with targeted GF ligands (antibodies, aptamers, or peptides). The potential of synthetic platforms with abiotic affinity as cost-effective alternatives to the current biological ligands is also discussed. Overall, the various GF sequestering systems developed so far have remarkably improved the activity of GFs at reduced doses and, in some cases, completely avoided the need for their exogenous administration to guide cell fates. These bioinspired concepts thus enable the rational exploration of the full therapeutic potential of GFs in regenerative medicine.

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Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 93%

Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 99%

Section: Platforms With Inclusion Of Ecm Componentsmentioning

confidence: 99%

“…Remarkably, this behavior of FN on PEA surfaces is highly specific, even when compared to other closely related control polymers such as poly(methyl acrylate). [ 93,127–129 ]…”

Section: Solid‐phase Presentationmentioning

confidence: 99%

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Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Teixeira

Domingues

Shevchuk

et al. 2020

Adv Funct Materials

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“…Understanding macromolecules adsorption process is of major significance in a variety of fields, such as surface treatment, lubrication, the pharmaceutical and cosmetic industries as well as in biological processes and biophysics . The adsorption of macromolecules (e.g., proteins) on biomaterials such as polymers, ceramics, or metals is of paramount importance for tissue engineering and regenerative medicine applications . Furthermore, knowledge of the structural formation at the solid/liquid interface is also a key factor in designing micro/nanostructures with promising applications in nanotechnology …”

Section: Introductionmentioning

confidence: 99%

Conformational Changes and Dynamics during Adsorption of Macromolecules with Different Degree of Polymerization Studied by Monte Carlo Simulations

Serra

Cabanilles

Dueñas

et al. 2018

Macro Theory & Simulations

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such as polymers, ceramics, or metals is of paramount importance for tissue engineering and regenerative medicine applications. [7][8][9][10] Furthermore, knowledge of the structural formation at the solid/liquid interface is also a key factor in designing micro/nanostructures with promising applications in nanotechnology. [11,12] The phase transition of diluted macromolecules in equilibrium is characterized by conformational changes. [13] Macromolecules diluted in a good solvent favor random coil conformations at high temperatures. When the temperature drops, the macromolecules collapse and adopt a globular conformation with lower conformational entropy than coil structures. However, globular conformation is entropy-dominated and the system evolves toward crystalline conformations. [14][15][16] The presence of an attractive surface could assist adsorbed chains toward crystalline or nematic order, leading to configurations that do not occur on a nonadherent surface. [13,17,18] Reduced motion of a polymer chain is commonly found near the surface during adsorption. The attraction potential between the surface and the monomers is fundamental for the polymer chain to adsorb and remain on the surface. Concentration, temperature, and chain length influence the dynamics of the adsorbed polymer chains. [19] Meta-stable polymer states play an important role in polymer physics. Mainly dependent (though not exclusively) on temperature, the mobility of polymer chains is at times so hindered that meta-stable conformations remain over long periods of time. Both experiments [20] and simulations [21] have revealed the existence of thermodynamically meta-stable states during the coil-to-globule transition. This constraint on mobility produces the glass transition and structural relaxation phenomena. In the glass transition temperature range, the chains become frozen in an out-of-equilibrium state in which conformational motions are severely limited and only local movements are allowed. Below the glass transition temperature the system evolves toward equilibrium in what is known as structural relaxation. [22,23] Molecular simulations such as molecular dynamics [24][25][26][27] and coarse-grained models [28][29][30][31] have been extensively used to analyze both the static and dynamic properties of polymeric materials. By simplifying detailed chemistry and atomic details, Monte Carlo SimulationsDynamics of multilayer adsorption of macromolecules with different degree of polymerization is studied by coarse-grained Monte Carlo simulations, focusing on both the interface macromolecule-surface and chain-chain interaction in and out of equilibrium conditions. The interfacial interaction between the solid flat surface and the macromolecules is modeled by means of a Lennard-Jones potential, and inter-and intrachain interactions are simulated using bond angle, bond length, and Lennard-Jones potentials. The results show that local and conformational motions near the glass transition temperature promote configurations with minimal energy, wh...

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Vitronectin as a Micromanager of Cell Response in Material‐Driven Fibronectin Nanonetworks

et al. 2017

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Surface functionalization strategies of synthetic materials for regenerative medicine applications comprise the development of microenvironments that recapitulate the physical and biochemical cues of physiological extracellular matrices. In this context, material‐driven fibronectin (FN) nanonetworks obtained from the adsorption of the protein on poly(ethyl acrylate) provide a robust system to control cell behavior, particularly to enhance differentiation. This study aims at augmenting the complexity of these fibrillar matrices by introducing vitronectin, a lower‐molecular‐weight multifunctional glycoprotein and main adhesive component of serum. A cooperative effect during co‐adsorption of the proteins is observed, as the addition of vitronectin leads to increased fibronectin adsorption, improved fibril formation, and enhanced vitronectin exposure. The mobility of the protein at the material interface increases, and this, in turn, facilitates the reorganization of the adsorbed FN by cells. Furthermore, the interplay between interface mobility and engagement of vitronectin receptors controls the level of cell fusion and the degree of cell differentiation. Ultimately, this work reveals that substrate‐induced protein interfaces resulting from the cooperative adsorption of fibronectin and vitronectin fine‐tune cell behavior, as vitronectin micromanages the local properties of the microenvironment and consequently short‐term cell response to the protein interface and higher order cellular functions such as differentiation.

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Role of chemical crosslinking in material-driven assembly of fibronectin (nano)networks: 2D surfaces and 3D scaffolds

Abstract: Graphical abstract

Cited by 13 publications

References 60 publications

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Biomaterials for Sequestration of Growth Factors and Modulation of Cell Behavior

Conformational Changes and Dynamics during Adsorption of Macromolecules with Different Degree of Polymerization Studied by Monte Carlo Simulations

Vitronectin as a Micromanager of Cell Response in Material‐Driven Fibronectin Nanonetworks

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