Role of chilling in the acquisition of cold tolerance and the capacitation to express stress proteins in diapausing pharate larvae of the gypsy moth, <i>Lymantria dispar</i>

Denlinger, David L.; Lee, Richard; Yocum, George D.; Kukal, Olga

doi:10.1002/arch.940210404

Cited by 59 publications

(44 citation statements)

References 31 publications

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“…Thus, none of the heat-shock-responsive genes are regulated as a function of diapause, suggesting that they are not involved in the expression of diapause in D. triauraria. In insects, regulation of HSPs in response to diapause has been studied only in four species, D. triauraria (Goto et al, 1998; the present study), S. crassipalpis (Yocum et al, 1998;, gypsy moth Lymantria dispar (Yocum et al, 1991;Denlinger et al, 1992) and Colorado potato beetle Leptinotarsa decemlineata (Yocum, 2001). The last two species also represent different responses from S. crassipalpis at least for the regulation of Hsp70.…”

Section: Discussionmentioning

confidence: 92%

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Heat-shock-responsive genes are not involved in the adult diapause of Drosophila triauraria

Goto

Kimura

2004

Gene

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Although the molecular regulation of diapause remains largely unknown, there is an accumulation of data suggesting the involvement of heat-shock proteins in the expression of diapause or dormancy. However, Goto et al. [J. Insect Physiol. 44 (1998) 1009] reported that Drosophila triauraria does not express Hsp70 transcripts at normal temperatures, regardless of the diapause state. Here, we investigated the transcriptional regulation of other heat-shock-responsive genes (Hsp23, Hsp26, Hsp83 and Hsrw) in D. triauraria with relation to diapause. The results revealed that these genes are not regulated as a function of diapause, suggesting that they are not involved in the expression of diapause in this species.

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Section: Discussionmentioning

confidence: 92%

“…HSP70 is not expressed upon the initiation of diapause but only after cold temperature has been realized in Ly. dispar (Yocum et al, 1991;Denlinger et al, 1992). On the other hand, in Le.…”

Section: Discussionmentioning

confidence: 94%

Heat-shock-responsive genes are not involved in the adult diapause of Drosophila triauraria

Goto

Kimura

2004

Gene

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“…Studies on sHsp regulation in dormancies of different organisms revealed heterogenous patterns (Bonato et al 1987;Yocum et al 1991;Denlinger et al 1992;Liang et al 1997a;Yocum et al 1998;Tammariello et al 1999;Cherkasova et al 2000;Goto and Kimura 2004;Rinehart et al 2007;Gkouvitsas et al 2008), indicating a diverse array of functions. An essential upregulation of shsp has been suggested for cold hardiness in the flesh fly Sarcophaga crassipalpis (Rinehart et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Stress response in tardigrades: differential gene expression of molecular chaperones

Reuner

Hengherr

Mali

et al. 2010

Cell Stress and Chaperones

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Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (MtsHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.

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“…In Vrba et al (2012) the larvae were simply acclimated to constant 5°C. Assaying the cold hardiness of animals acclimated to a constant 5°C is frequently used in cold hardiness studies, as it facilitates rapid inter-species comparisons (Denlinger et al, 1992). In natural settings, however, external conditions change during winter, causing the changes in SCP values recorded here and elsewhere .…”

Section: Variation In Cold Hardiness and Cryoprotectant Contentmentioning

confidence: 99%

More complex than expected: Cold hardiness and the concentration of cryoprotectants in overwintering larvae of five Erebia butterflies (Lepidoptera: Nymphalidae)

Vrba¹,

Nedvěd²,

Zahradníčková³

et al. 2017

Eur. J. Entomol.

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Abstract. Understanding the factors restricting the distribution of some insect species to high altitudes is hindered by poor knowledge of temporal changes in their cold hardiness during overwintering. We studied overwintering larvae of fi ve species of Erebia butterfl ies (Lepidoptera: Nymphalidae: Satyrinae) differing in altitudinal distribution: lowland E. medusa, submountain E. aethiops, subalpine E. pronoe, alpine E. cassioides, and subnivean E. pluto. We subjected them to three treatments, AutumnWarm (13/8°C), imitating conditions prior to overwintering; AutumnCold (5/0°C), imitating late autumn conditions; and WinterCold (5/0°C), differing from AutumnCold by a shorter photoperiod and longer exposure to zero temperatures. Supercooling points (SCP) did not differ between species in the AutumnWarm treatment, despite large differences in the concentrations of cryoprotectants (CrPC; lowest in E. medusa and E. aethiops). Lowland E. medusa was freeze-tolerant, the subalpine, alpine and subnivean species were freezeavoidant, whereas submountain E. aethiops displayed a mixed strategy. SCPs diverged in the AutumnCold treatment: it increased in the lowland E. medusa (from -16.5 to -10.8°C) and reached the lowest value in E. cassioides (-21.7°C). In WinterCold, SCP increased in subalpine E. pronoe (from -16.1°C in AutumnWarm and -18.7°C in AutumnCold to -12.6°C). E. medusa decreased and E. aethiops increased their CrPCs between autumn and winter; the highest CrPC was recorded in subnivean E. pluto. CrPC did not correlate with SCP across species and treatments. Cryoprotectant profi les corroborated the difference between lowland and freeze-tolerant E. medusa and the three high altitude freeze-avoidant species, with E. aethiops in an intermediate position. Glycerol was surprisingly rare, trehalose was important in all species, and such rare compounds as monopalmitin and monostearin were abundantly present in E. pronoe, E. cassioides and E. pluto.

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Role of chilling in the acquisition of cold tolerance and the capacitation to express stress proteins in diapausing pharate larvae of the gypsy moth, Lymantria dispar

Cited by 59 publications

References 31 publications

Heat-shock-responsive genes are not involved in the adult diapause of Drosophila triauraria

Heat-shock-responsive genes are not involved in the adult diapause of Drosophila triauraria

Stress response in tardigrades: differential gene expression of molecular chaperones

More complex than expected: Cold hardiness and the concentration of cryoprotectants in overwintering larvae of five Erebia butterflies (Lepidoptera: Nymphalidae)

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