Role of cold atmospheric plasma in microbial inactivation and the factors affecting its efficacy

Das, Sarthak; Prakash, G. Veda; Mohapatra, Sarita; Singh, Gagandeep; Kar, Satyananda

doi:10.1016/j.hsr.2022.100037

Cited by 43 publications

(40 citation statements)

References 115 publications

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“…along with H2O2, O I, O2 -, and ONOOmight directly attack the bacterial cell membrane constituting lipid bilayer and proteins. These species initiate lipid peroxidation and protein oxidation by the hydrogen atom abstraction mechanism, resulting in the loss of structural integrity in bacteria [17], [49]- [52]. Also, the interaction of gas phase species (RONS, excited species, and ions) with the embedded water in the solid media results in the formation of secondary RONS (OH .…”

Section: Discussionmentioning

confidence: 99%

“…Further, a variation in inactivation among all the isolates at greater than 10 2 CFU/ ml (e.g., at 10 5 CFU/ml and 300 s CAP exposure, inactivation was 78.56 % for E. coli, 63.30 % for S. aureus, etc.) might be due to the structural difference between different bacterial isolates [17], [53], [54], [59], [60], thus CAP susceptibility varies. Additionally, a significant difference in inactivation area between the E. coli (Gram-negative bacillus) and S. aureus (Grampositive cocci) was noticed on variable CAP exposure time and distance.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, a significant difference in inactivation area between the E. coli (Gram-negative bacillus) and S. aureus (Grampositive cocci) was noticed on variable CAP exposure time and distance. The peptidoglycan layer of the bacterial cell wall distinguishes Gram-positive and Gram-negative [17], [54]. The Gram-positive bacteria have a peptidoglycan thickness of 30-100 nm, whereas Gram-negative bacteria have a thickness of 1-8 nm [53], [54], [60].…”

Section: Discussionmentioning

confidence: 99%

“…, etc. that plays a crucial role in the microbial inactivation by CAP [17]- [24]. The antimicrobial efficacy of CAP has been investigated invitro on various bacteria such as E. coli (from Family of Enterobacteria) [25]- [28], S. aureus [27]- [31], P. aeruginosa [28]- [33], K. pneumoniae [30], [31], A. baumannii [30], [34], etc.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antimicrobial Efficacy of Argon Cold Atmospheric Pressure Plasma Jet on Clinical Isolates of Multidrug-Resistant ESKAPE Bacteria

Das

Prakash

Mohapatra

et al. 2023

IEEE Trans. Radiat. Plasma Med. Sci.

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The rise in multidrug-resistant (MDR) ESKAPE bacteria have become a major therapeutic challenge globally. Recently, novel cold atmospheric pressure plasma (CAP) as an antimicrobial is becoming popular. In this study, an indigenously developed AC cold atmospheric pressure plasma jet (CAPJ) fed with argon gas was used to evaluate its antimicrobial efficacy on these bacteria isolated from clinical specimens such as urine, blood, and sputum in a tertiary care hospital in India. The difference in CAP's antimicrobial activity on Gram-negative bacilli (MDR E. coli) and Grampositive cocci (MDR S. aureus) was observed with various input parameters, such as microbial concentration, CAP exposure time, and exposure distance. It was observed that oxidative stress induced by reactive oxygen and nitrogen species (O2 -, NO + , OH . , H2O2, ONOO -, NO . , NO2 . , HO2 . , O3 -, etc.) and electrostatic stress by ions (Ar + , O + , O2 -, OH -, NO + , OH + , NO3 -, NO2 -, O3 -, etc.) might play a crucial role in microbial inactivation. In addition to this, a decrease in adenosine triphosphate concentration post-CAP exposure in a liquid media suggested an efficient microbial inactivation effect. The outcome of this research would be extremely beneficial to multidisciplinary researchers in this field.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial Efficacy of Argon Cold Atmospheric Pressure Plasma Jet on Clinical Isolates of Multidrug-Resistant ESKAPE Bacteria

Das

Prakash

Mohapatra

et al. 2023

IEEE Trans. Radiat. Plasma Med. Sci.

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“…In the recent past, the role of different input parameters that the effect on bactericidal efficacy of LTAP has been discussed in detail as a review [27,28]. De Baerdemaeker et al [29] have come up with an innovative way of plasma mesh for the bactericidal efficacy of Salmonella Typhimurium but did not optimize the input parameters of the LTAP.…”

Section: Sidhartha and Sanjay Have Equal Contributionmentioning

confidence: 99%

The quality by design approach for optimization of slayer exciter based low power portable atmospheric plasma jet on bactericidal efficacy of Pseudomonas aeruginosa

Sidhartha

Ghosh

et al. 2023

Journal of Biophotonics

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A simple, portable, economical lowtemperature atmospheric plasma (LTAP) for bactericidal efficacy of Gram-negative bacteria (Pseudomonas aeruginosa) with different carrier gases (argon, helium, and nitrogen) using the quality by design (QbD) approach, design of experiments (DoE), and response surface graphs (RSG) is presented. Box-Behnken design was used as the DoE to narrow down and further optimize the experimental factors of LTAP. Plasma exposure time, input DC voltage, and carrier gas flow rate were varied to examine the bactericidal efficacy using the zone of inhibition (ZOI). A higher bactericidal efficacy was achieved under the optimal bactericidal factors having ZOI of 50.837 ± 2.418 mm 2 with the plasma power density of 132 mW/cm 3 for LTAP-Ar at 61.19 s, 14.8747 V, and 219.379 sccm than LTAP-He and LTAP-N 2 . The LTAP-Ar was further evaluated at different frequencies and probe lengths to achieve a ZOI of 58.237 ± 4.01 mm 2 .

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Microwave atmospheric pressure plasma jet: A review

Rath,

Kar

2024

Contrib. Plasma Phys

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Considerable interest has emerged in atmospheric pressure discharges within the microwave frequency range over the past decade, driven by the growing potential applications such as material processing, CO2 dissociation, waste treatment, hydrogen production, water treatment, and so forth. This review delves into the diverse types of atmospheric pressure plasma jets (APPJs) operated at microwave frequencies. The analysis integrates insights from an overall review that encapsulates the different types of geometry, characterizations, modeling, and various applications of microwave atmospheric plasma jets (MW‐APPJs). This paper will contribute to a comprehensive understanding of microwave plasma generated in the ambient atmosphere. The fundamental insights into these discharges are emerging, but there are still numerous unexplained phenomena in these inherently complex plasmas that need to be studied. The properties of these MW‐APPJs encompass a higher range of electron densities (ne), gas temperatures (Tg), electron temperatures (Te), and reactive oxygen and nitrogen species (RONS). This review provides an overview of the key underlying processes crucial for generating and stabilizing MW‐APPJs. Additionally, the unique physical and chemical properties of these discharges are summarized. In the initial section, we aim to introduce the primary scientific characterizations of different types of waveguide‐based and non‐waveguide‐based MW‐APPJs. The subsequent part focuses on the diverse modeling approaches for different MW‐APPJs and the outcomes derived from these models. The final section describes the potential applications of MW‐APPJs in various domains.

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Role of cold atmospheric plasma in microbial inactivation and the factors affecting its efficacy

Cited by 43 publications

References 115 publications

Antimicrobial Efficacy of Argon Cold Atmospheric Pressure Plasma Jet on Clinical Isolates of Multidrug-Resistant ESKAPE Bacteria

Antimicrobial Efficacy of Argon Cold Atmospheric Pressure Plasma Jet on Clinical Isolates of Multidrug-Resistant ESKAPE Bacteria

The quality by design approach for optimization of slayer exciter based low power portable atmospheric plasma jet on bactericidal efficacy of Pseudomonas aeruginosa

Microwave atmospheric pressure plasma jet: A review

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