Role of collagenase in colonic anastomoses: a reappraisal

Abstract

Increased collagenolysis, with reduction in collagen concentration, has been incriminated in the breakdown of colonic anastomoses but previous studies have measured only collagen levels and non-specific collagenolytic activity. Collagenase, the initiating enzyme in collagen degradation, is synthesized on demand and controlled by tissue inhibitor of metalloproteinases (TIMP). Antibodies to collagenase and TIMP were applied to colonic anastomoses in rabbits to investigate the role of the enzyme during healing. Within 12 h of operation, secreting cells and extracellular collagenase were identified at the everted edges of the bowel wall. After 24 h, collagenase activity was accompanied by TIMP secretion in the same localized regions, and by the third postoperative day very few cells were still synthesizing enzyme in these areas, although extracellular activity remained visible. TIMP-secreting cells, however, were seen in a layer of connective tissue sealing the serosal surface of the anastomosis. At 7 days, both enzyme and inhibitor were found only in small aggregates of secreting cells in the deeper layers. The localization and extent of collagenase and TIMP activity accorded well with a normal healing response as, at all times, the enzyme was confined to the immediate vicinity of the suture line.

Distinct localization of collagenase and tissue inhibitor of metalloproteinases expression in wound healing associated with ulcerative pyogenic granuloma.

“…These samples represent all cases presented at Washington University School of Medicine over a four year period. Of the 16 samples studied, 10 had inflammatory infiltration and ulceration (Table I). Of these, six were advanced lesions and four were less advanced as judged by the extent of stromal cellularity and neovascularization.…”

Divergent patterns of matrix metalloproteinase activity during wound healing in ileum and colon of rats.

“…After electrophoresis the gels were washed three times in 2.5% Triton X-100 (v/v) for 10 minutes at room temperature. After the gels were rinsed twice in a buffer containing 50 mM TRIS-HCI, 5 construction. It was also noted that the activity of the 60 and 51 kDa bands was increased with respect to uninjured colon.…”

Effect of thalidomide on the healing of colonic anastomosis, in rats

“…These enzymes are significantly expressed only at sites that need healing, especially along the suture lines, and do not appear in other parts of the tissue outside the necessary site. 42 Using histochemical techniques, the presence of metalloproteinases is detected as early as during the first 24 hours of anastomosis healing, especially in the extracellular matrix of the mucosa. After 72 hours, they are still present in the mucosa but they are also detected in deeper layers, especially in fibrous tissues, where their remodeling lytic effect is needed.…”