Role of CXCR7 and Effects on CXCL12 in SiHa Cells and Upregulation in Cervical Squamous Cell Carcinomas in Uighur Women

Kurban, Shajidai; Tursun, Mikrban; Kurban, Gulinar; Hasim, Ayshamgul

doi:10.7314/apjcp.2014.15.21.9211

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…Among 27 studies, three studies each evaluated colorectal carcinoma (or rectal cancer) 18 - 20 , hepatocellular carcinoma 21 - 23 , esophageal carcinoma (or esophageal squamous cell carcinoma) 13 , 24 , 25 , and thyroid carcinoma 26 - 28 . Two studies evaluated renal cell carcinoma 29 , 30 , pancreatic cancer 31 , 32 , cervical cancer (or cervical squamous cell carcinoma) 33 , 34 , nasopharyngeal carcinoma 35 , 36 , glioma or glioblastoma 37 , 38 and gastric cancer 39 , 40 . One study evaluated gallbladder cancer 14 , cutaneous squamous cell carcinoma 41 and chondrosarcoma 42 .…”

Section: Meta-analysis Resultsmentioning

confidence: 99%

Role of CXCR7 as a Common Predictor for Prognosis in Solid Tumors: a Meta-Analysis

Zhao¹,

Zhang²,

Qin³

et al. 2018

J. Cancer

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Background: Accumulating evidence indicated that the CXC chemokine receptor (CXCR) 7 (CXCR7) was overexpressed in a variety of tumors. However, the value of the CXCR7 expression in predicting prognosis in solid tumors remains controversial. Therefore, we performed this meta-analysis to evaluate the correlation between CXCR7 expression and lymph node metastasis (LNM), tumor pathological grade and survival, including overall survival (OS), disease-free survival (DFS) and recurrence-free survival (RFS).Methods: Eligible studies were searched in PubMed, Web of Science, and PMC up to April 2018. A total of 27 studies were included in this meta-analysis. Odds ratio (OR), hazard ratio (HR) and 95 % confidence intervals (CI) were used as effect measures.Results: The meta-analysis showed that high expression of CXCR7 predicted a high risk of LNM (pooled OR = 2.22, 95%CI: 1.41-3.50), high tumor grade (pooled OR = 1.94, 95%CI: 1.20-3.13), poor OS (pooled HR = 1.66, 95%CI: 1.30-2.03), and poor DFS/RFS (pooled HR = 1.82, 95%CI: 1.21-2.43). Subgroup analysis showed that CXCR7 expression had a positive correlation with LNM in pan-adenocarcinoma subgroup (pooled OR = 3.73, 95%CI: 2.21-6.30), while no correlation was found in pan-squamous cancer subgroup (pooled OR = 1.29, 95%CI: 0.56-2.96). Subgroup analysis of tumor grade revealed that high expression of CXCR7 predicted high tumor grade both in pan-squamous cancer and pan-adenocarcinoma (pooled OR = 3.58, 95%CI: 1.39-9.22, pooled OR = 2.25, 95%CI: 1.20-4.20). As in OS group, we divided the data based on analysis method and it turned out that overexpressed CXCR7 predicted worse OS both in multivariate analysis (pooled HR =1.57, 95%CI: 1.12-2.01) and univariate analysis subgroup (pooled HR =1.86, 95%CI: 1.23-2.49).Conclusions: Our meta-analysis revealed that high expression of CXCR7 predicted unfavorable prognosis and may serve as potential targets of cancer therapy.

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Section: Meta-analysis Resultsmentioning

confidence: 99%

Role of CXCR7 as a Common Predictor for Prognosis in Solid Tumors: a Meta-Analysis

Zhao¹,

Zhang²,

Qin³

et al. 2018

J. Cancer

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“…Previously, it was reported that CXCR7 expression was higher in cervical cancer compared to CIN and normal cervical mucosa, particularly in those with an advanced stage of cancer and lymph node metastasis (10). However, the difference in CXCR7 protein expression among different stages of CIN has not been previously investigated and the ROC curve analysis has not been performed.…”

Section: Discussionmentioning

confidence: 97%

CXC chemokine receptor 7 expression in cervical intraepithelial neoplasia

Tang

Xia

2015

Biomedical Reports

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Abstract. Cervical intraepithelial neoplasia (CIN), also known as transformation and dysplasia of cervical intraepithelial cells, is the precancerous lesion of squamous cell carcinoma. CXC chemokine receptor 7 (CXCR7) has been indicated in tumor development and metastasis of multiple malignancies or precancerous lesion. However, the protein expression and function of CXCR7 in different stages of human CIN remains unclear. The present study examined CXCR7 protein expression in cervical tissue samples from 34 patients, including 7 patients with normal cervical tissues (negative control), 10 patients with stage I of CIN (CIN I), 8 patients with CIN II and 9 patients with CIN III. Receiver operating characteristic curves (ROC) were established to evaluate the prognostic value of CXCR7 in differentiating various stages of CIN. Immunohistochemical staining showed that protein expression of CXCR7 was higher in CIN tissues compared with the normal cervical epithelium (P<0.05). High-grade CIN tissues expressed a higher level of CXCR7 compared to low-grade samples. The ROC curve of integral optical density analysis showed that CXCR7 could discriminate CIN I-III from normal cervical epithelium with 88.9% sensitivity and 71.4% specificity, and CIN II-III from the negative control and CIN I with 92.7% sensitivity and 50.0% specificity. ROC curve of area analysis also showed that CXCR7 could discriminate CIN I-III from normal cervical epithelium with 70.4% sensitivity and 100.0% specificity, and CIN II-III from the negative control and CIN I with 50.0% sensitivity and 90.0% specificity. An increase in CXCR7 expression may represent a novel predictor of CIN. The wide expression of CXCR7 in CIN also supports the assumption that CXCR7 plays a role in precancerous lesion progression, as well as proliferation, migration and angiogenesis. IntroductionCervical intraepithelial neoplasia (CIN), also known as transformation and dysplasia of cervical intraepithelial cells, is the precancerous lesion of squamous cell carcinoma. CIN is usually detected through the Papanicolaou (Pap) cytological screening or the high-risk human papillomavirus (HPV) DNA testing (1). However, the Pap test or superior colposcope multipoint sampling requires gynecological surgery. The current inspection method takes time and may fail to collect the most severe lesions. The diagnosis and staging of collected specimens mainly depends on the pathological features.The mechanisms involved in cell transformation and dysplasia of CIN remain unclear. CXC chemokine receptor type 7 (CXCR7) has been suggested to play a role in tumor development. However, studies on the function of CXCR7 in precancerous lesions are limited. CXCR7 is a non-G protein-coupled receptor. Heterodimerization of CXCR7 with other receptors, such as the epidermal growth factor receptor or CXCR4, triggers intracellular signaling events that are important for the regulation of cell proliferation and apoptosis (2-4). Expression of CXCR7 has been associated with a higher tumor grade and more aggressive...

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“…The present study utilized SiHa cervical carcinoma cell line, which was described as a squamous carcinoma cell line 22 and exhibited medium level of Twist1 expression among four cervical cell lines (data not shown). Previous study indicated that HIF-1α could bind directly to the hypoxia-response element (HRE) in Twist1 promoter to enhance Twist1 expression 9 .…”

Section: Discussionmentioning

confidence: 99%

Twist1 Enhances Hypoxia Induced Radioresistance in Cervical Cancer Cells by Promoting Nuclear EGFR Localization

et al. 2017

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Twist1 is a crucial transcription factor that regulates epithelial mesenchymal transition and involves in metastasis. Recent evidence suggests that Twist1 plays important role in hypoxia-induced radioresistance, but the underlying mechanism remains elusive. Here we investigated the change of Twist1 expression in human cervical squamous cancer cell line SiHa after hypoxia treatment. We also explored the role of Twist1 in radioresistance by manipulating the expression level of Twist1. We observed that hypoxia treatment elevated the expression of Twist1 in SiHa cells. Knockdown of Twist1 with siRNA increased the radiosensitivity of SiHa cells under hypoxia condition, accompanied by reduced levels of nuclear Epidermal Growth Factor Receptor (EGFR) and DNA-dependent protein kinase (DNA-PK). Conversely, overexpression of Twist1 led to increased radioresistance of SiHa cells, which in turn increased nuclear EGFR localization and expression levels of nuclear DNA-PK. Moreover, concomitant high expression of hypoxia-inducible factor-1α (HIF-1α) and Twist1 in primary tumors of cervical cancer patients correlated with the worse prognosis after irradiation treatment. Taken together, these data provide new insights into molecular mechanism underlying hypoxia-induced radioresistance in cervical cancer cells, and suggest that Twist1 is a promising molecular target to improve the efficacy of cancer radiotherapy.

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Role of CXCR7 and Effects on CXCL12 in SiHa Cells and Upregulation in Cervical Squamous Cell Carcinomas in Uighur Women

Cited by 7 publications

References 16 publications

Role of CXCR7 as a Common Predictor for Prognosis in Solid Tumors: a Meta-Analysis

Role of CXCR7 as a Common Predictor for Prognosis in Solid Tumors: a Meta-Analysis

CXC chemokine receptor 7 expression in cervical intraepithelial neoplasia

Twist1 Enhances Hypoxia Induced Radioresistance in Cervical Cancer Cells by Promoting Nuclear EGFR Localization

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