Role of cyanogenic glycosides in the seeds of wild lima bean, Phaseolus lunatus: defense, plant nutrition or both?

Cuny, Maximilien A. C.; Forgia, Diana La; Desurmont, Gaylord A.; Glauser, Gaëtan; Benrey, Betty

doi:10.1007/s00425-019-03221-3

Cited by 14 publications

(8 citation statements)

References 76 publications

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“…For example, glucosinolates in Arabidopsis thaliana vary among plant tissues and the highest concentrations are found in seedlings (Hopkins et al, 2009). Similarly, in lima bean (Phaseolus lunatus), cyanogenic glycosides are found in higher quantity in cotyledons than in other plant vegetative tissues (Cuny et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Squash Varieties Domesticated for Different Purposes Differ in Chemical and Physical Defense Against Leaf and Root Herbivores

et al. 2021

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Plant domestication often reduces plant defenses by selection on chemical and physical defense traits. Thus, it is expected that herbivorous insects perform better on crop varieties than on their wild relatives. However, recent studies show that this pattern is not ubiquitous. We examined how varieties of squash (Cucurbita spp.) domesticated for different purposes (for consumption or as ornamentals), differ in plant defense traits and in their interactions with a leaf and a root herbivore. Two types of defenses were measured: cucurbitacins, which are toxic phytochemicals, and trichomes that are physical barriers for most herbivores. We addressed the following questions: (i) what is the variation in cucurbitacin content and leaf trichome density among varieties? (ii) does purpose of domestication explain differences in defense traits among varieties? and (iii) are herbivore feeding preferences and performance altered by the defense traits of squash varieties? We found great variation in cucurbitacin content among varieties, but not according to their purpose of domestication. Cucurbitacins were found mostly in cotyledons and roots and in very small quantities in the leaves. In contrast, trichome density was greater on the varieties selected for consumption than on the ornamental varieties. The performance of a leaf herbivore (Spodoptera latifascia) and a root herbivore (Diabrotica balteata), was not different among squash varieties. Moreover, in a choice experiment, larvae of the root herbivore preferred to feed on squash varieties with high cucurbitacin content. Whereas, in the field, native leaf herbivores preferred to feed on varieties selected for consumption. Our results contribute to a better understanding on how varietal selection may differentially affect plant defenses. This knowledge could help in the development of crop varieties with enhanced insect resistance.

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Section: Discussionmentioning

confidence: 99%

Squash Varieties Domesticated for Different Purposes Differ in Chemical and Physical Defense Against Leaf and Root Herbivores

et al. 2021

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“…Besides their defensive function, CNglcs have also been involved in plant growth and development and as osmo-protectants and scavengers or reactive oxygen species associated with responses to adverse environments [ 30 , 31 ]. Finally, CNglcs also play an important role in the transportation and turnover of nitrogen and glucose during specific plant developmental stages [ 31 ].…”

Section: Metabolic Pathways Linked To Cyanogenesismentioning

confidence: 99%

Cyanogenesis, a Plant Defence Strategy against Herbivores

Botër

Dı́az

2023

IJMS

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Plants and phytophagous arthropods have coevolved in a long battle for survival. Plants respond to phytophagous feeders by producing a battery of antiherbivore chemical defences, while herbivores try to adapt to their hosts by attenuating the toxic effect of the defence compounds. Cyanogenic glucosides are a widespread group of defence chemicals that come from cyanogenic plants. Among the non-cyanogenic ones, the Brassicaceae family has evolved an alternative cyanogenic pathway to produce cyanohydrin as a way to expand defences. When a plant tissue is disrupted by an herbivore attack, cyanogenic substrates are brought into contact with degrading enzymes that cause the release of toxic hydrogen cyanide and derived carbonyl compounds. In this review, we focus our attention on the plant metabolic pathways linked to cyanogenesis to generate cyanide. It also highlights the role of cyanogenesis as a key defence mechanism of plants to fight against herbivore arthropods, and we discuss the potential of cyanogenesis-derived molecules as alternative strategies for pest control.

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“…Cyanogenic glycosides (CNGs) are a group of secondary metabolites of the plant kingdom involved in different functions, such as the transport and turnover of nitrogen and plant development [1].…”

Section: Introductionmentioning

confidence: 99%

“…The analytical methods described in the literature to quantify CNG from mandelonitrile usually employ water, methanol or their mixtures, and different extraction systems such as Soxhlet extraction, solid-phase extraction, ultrasound bath or solid-liquid extraction assisted by microwaves [1,2,14,17,[20][21][22] during extraction periods that may last for 24 h. The diverse chemical nature of cyanogenic glycosides means that the extraction and analysis of individual compounds can be difficult and furthermore, degradation can be rapid under appropriate conditions [16].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Cyanogenic Compounds Derived from Mandelonitrile by Ultrasound-Assisted Extraction and High-Performance Liquid Chromatography in Rosaceae and Sambucus Families

Madrera

Valles

2021

Molecules

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An analytical method for extraction and quantitative determination of amygdalin, prunasin, and sambunigrin in plant material is described. The method is based on extraction with high-power ultrasound (UAE), with acidified water as solvent and quantification by HPLC–DAD. The best extraction conditions were: 80% sonication amplitude, 55 s extraction time, 70% duty cycle, 0.1 g sample mass, and 10 mL acidified water (0.1% perchloric acid). Once developed, the method was validated in terms of accuracy and precision. Good linearity was obtained, with correlation coefficients exceeding 0.999 and the quantification limits ranged from 2.2 μg/g (amygdalin) to 9.6 μg/g (sambunigrin). The accuracy (recovery study) ranged between 90 and 104% and the reproducibility of the method was always <2.3% (RSD). Special attention should be paid to the ratio sample/solvent in samples with potential β-glucosidase activity to avoid degradation of the cyanogenic glycosides (CNGs). The proposed method was used to evaluate the content of CNGs in kernels of Prunus genera, apple seeds, apple pomace, and different plant materials of Sambucus nigra.

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Role of cyanogenic glycosides in the seeds of wild lima bean, Phaseolus lunatus: defense, plant nutrition or both?

Cited by 14 publications

References 76 publications

Squash Varieties Domesticated for Different Purposes Differ in Chemical and Physical Defense Against Leaf and Root Herbivores

Squash Varieties Domesticated for Different Purposes Differ in Chemical and Physical Defense Against Leaf and Root Herbivores

Cyanogenesis, a Plant Defence Strategy against Herbivores

Analysis of Cyanogenic Compounds Derived from Mandelonitrile by Ultrasound-Assisted Extraction and High-Performance Liquid Chromatography in Rosaceae and Sambucus Families

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