Role of cyclic nucleotide signaling in oocyte maturation

Conti, Marco; Andersen, Carsten; Richard, François J.; Méhats, Céline; Chun, Sang-Young; Horner, Kathleen; Jin, Catherine; Tsafriri, A.

doi:10.1016/s0303-7207(01)00686-4

Cited by 281 publications

(211 citation statements)

References 54 publications

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“…For example, during IVM, optimal cilostamide concentrations were 1 μM (sheep, current study) 20 μM (cattle) and 0.1 μM (mouse) [13]. This was expected due to differences among species in PDE kinetics, the presence of other PDE subtypes within the oocyte, and varying concentrations of endogenous oocyte cAMP production [46]. In the pre-IVM phase, 10-fold higher concentrations of IBMX were needed in bovine compared to murine models (500 versus 50 μM, respectively), perhaps due to expression of PDE8 in bovine COCs, which accounted for more than 20% of total PDE activity and is not inhibited by IBMX [11].…”

Section: Discussionmentioning

confidence: 72%

Regulation of sheep oocyte maturation using cAMP modulators

et al. 2013

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Section: Discussionmentioning

confidence: 72%

Regulation of sheep oocyte maturation using cAMP modulators

et al. 2013

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“…In that system, treatment with the uncoupler 1-heptanol induced abrupt chromatin condensation and decreased in transcription. However, the addition of the oocyte-specific PDE3 inhibitor cilostamide [31][32][33] to the culture medium nullified this effect indicating that the functional status of GJC may affect both transcriptional activity and remodeling of largescale chromatin configuration in growing oocytes, potentially through cAMP-dependent mechanism(s) [19].…”

Section: Discussionmentioning

confidence: 99%

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Lodde

Franciosi

Tessaro

et al. 2013

J Assist Reprod Genet

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Purpose This study was aimed to test the hypothesis that gap junction mediated communications (GJC) are required to allow the progressive chromatin configuration remodeling (from GV1 to GV3) process to occur in fully grown oocytes in order to gain the final step of developmental competence acquisition, and that a premature disruption of GJC can alter this process. Methods Bovine cumulus-oocytes complexes collected from medium antral follicles were cultured for 2, 4, 6 and 8 h in the presence of 10 −4 IU/ml of r-hFSH and with 2 mM of the nonselective PDE inhibitor 3-isobutyl-1-methyl-xanthine (IBMX) to prevent meiotic resumption. GJC functionality and chromatin configuration were monitored during the culture period. After meiotic arrest, the developmental capability of oocytes was assessed after IVM and IVF. Results IBMX was effective in significantly sustaining GJC up to 6 h and maintaining meiotic arrest, when compared to control group. Moreover, the percentage of oocytes with less condensed chromatin (GV1) decreased within 4 h of culture, while the proportion of GV2 oocytes gradually increased up to 6 h.Interestingly, a decline in the proportion of GV2 oocytes and an increase in the proportion of GV3 oocytes were observed after 6 h of culture, when the major drop of GJC occurred. On the contrary, when GJC were uncoupled by adding 3 mM of 1-heptanol or through cumulus cells removal, chromatin condensation occurred rapidly throughout the culture period, more promptly in denuded oocytes. Moreover, the maintenance of GJC during meiotic arrest was accompanied by a significant increase of developmental competence compared to the control, as indicated by a higher percentage of hatched blastocysts and blastocyst cell number. Conclusions Altogether, our data indicate that both paracrine and junctional mechanisms are involved in modulating largescale chromatin structure during the final phase of oocyte differentiation.

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“…Despite the ability of the fully grown oocyte to mature, it remains arrested in prophase I until the LH surge. It is well established that meiotic arrest is regulated by cAMP levels within the oocyte (Conti et al 2002, Eppig et al 2004. Spontaneous maturation of oocytes isolated from their follicles can be prevented by including membrane permeant cAMP analogs or cAMP phosphodiesterase inhibitors, such as hypoxanthine or 3-isobutyl-1-methylxanthine (IBMX), in the culture medium (Cho et al 1974, Dekel & Beers 1978, Conti et al 2002.…”

Section: Maintenance Of Meiotic Arrestmentioning

confidence: 99%

“…It is well established that meiotic arrest is regulated by cAMP levels within the oocyte (Conti et al 2002, Eppig et al 2004. Spontaneous maturation of oocytes isolated from their follicles can be prevented by including membrane permeant cAMP analogs or cAMP phosphodiesterase inhibitors, such as hypoxanthine or 3-isobutyl-1-methylxanthine (IBMX), in the culture medium (Cho et al 1974, Dekel & Beers 1978, Conti et al 2002. Moreover, cAMP levels decrease in oocytes following removal from their follicles (Tö rnell et al 1990), as well as in isolated oocytes after removal of IBMX (Schultz et al 1983a, Vivarelli et al 1983.…”

Section: Maintenance Of Meiotic Arrestmentioning

confidence: 99%

“…It is therefore possible that a lipid present in the regions of membrane contact between cumulus cells and oocyte stimulates GPR3. Another possibility for how the follicle cells might keep the oocyte arrested in prophase I until the LH surge is that they may inhibit oocyte phosphodiesterase(s) (Conti et al 2002). Both of these possibilities need to be explored further to determine how the follicle cells interact with the oocyte to keep cAMP levels high prior to the LH surge.…”

Section: Maintenance Of Meiotic Arrestmentioning

confidence: 99%

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Stops and starts in mammalian oocytes: recent advances in understanding the regulation of meiotic arrest and oocyte maturation

Mehlmann¹

2005

Reproduction

437

307

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Mammalian oocytes grow and undergo meiosis within ovarian follicles. Oocytes are arrested at the first meiotic prophase, held in meiotic arrest by the surrounding follicle cells until a surge of LH from the pituitary stimulates the immature oocyte to resume meiosis. Meiotic arrest depends on a high level of cAMP within the oocyte. This cAMP is generated by the oocyte, through the stimulation of the G s G-protein by the G-protein-coupled receptor, GPR3. Stimulation of meiotic maturation by LH occurs via its action on the surrounding somatic cells rather than on the oocyte itself. LH induces the expression of epidermal growth factor-like proteins in the mural granulosa cells that act on the cumulus cells to trigger oocyte maturation. The signaling pathway between the cumulus cells and the oocyte, however, remains unknown. This review focuses on recent studies highlighting the importance of the oocyte in producing cAMP to maintain arrest, and discusses possible targets at the level of the oocyte on which LH could act to stimulate meiotic resumption.

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Role of cyclic nucleotide signaling in oocyte maturation

Cited by 281 publications

References 54 publications

Regulation of sheep oocyte maturation using cAMP modulators

Regulation of sheep oocyte maturation using cAMP modulators

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Stops and starts in mammalian oocytes: recent advances in understanding the regulation of meiotic arrest and oocyte maturation

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