Role of CYP81A cytochrome P450s in clomazone metabolism in Echinochloa phyllopogon

Guo, Feng; Iwakami, Satoshi; Yamaguchi, Takuya; Uchino, Akira; Sunohara, Yukari; Matsumoto, Hiroshi

doi:10.1016/j.plantsci.2019.02.010

Cited by 54 publications

(102 citation statements)

References 35 publications

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“…and Guo et al . : sensitivity to an acetolactate synthase (ALS) herbicide (bensulfuron‐methyl), acetyl‐CoA carboxylase herbicides (diclofop‐methyl, tralkoxydim, and pinoxaden), and clomazone. (b) Association between quinclorac sensitivity and β‐CAS activity.…”

Section: Resultsmentioning

confidence: 99%

“…Attempts to elucidate the mechanisms of multiple herbicide resistance led to the identification of overexpressing herbicide‐metabolizing cytochrome P450 (P450) genes in resistant plants . The P450s CYP81A12 and CYP81A21 metabolize ALS inhibitors, ACCase inhibitors, and clomazone therefore confer multiple herbicide resistance …”

Section: Introductionmentioning

confidence: 99%

“…15 The P450s CYP81A12 and CYP81A21 metabolize ALS inhibitors, ACCase inhibitors, and clomazone therefore confer multiple herbicide resistance. 16,17 Quinclorac is a synthetic auxin herbicide with a quinolinecarboxylic acid backbone known to effectively control Poaceae weeds such as Echinochloa spp. As with other synthetic auxins, quinclorac is recognized by the F-box protein of the TIR1/AFB family of auxin receptors and a member of the Aux/IAA family of co-repressor proteins.…”

Section: Introductionmentioning

confidence: 99%

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Quinclorac resistance in Echinochloa phyllopogon is associated with reduced ethylene synthesis rather than enhanced cyanide detoxification by β‐cyanoalanine synthase

Chayapakdee

Sunohara

Endo

et al. 2019

Pest Management Science

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BACKGROUND Multiple herbicide resistant Echinochloa phyllopogon exhibits resistance to the auxin herbicide quinclorac. Previous research observed enhanced activity of the cyanide‐detoxifying enzyme β‐cyanoalanine synthase (β‐CAS) and reduced ethylene production in the resistant line, suggesting β‐CAS‐mediated cyanide detoxification and insensitivity to quinclorac stimulation as the resistance mechanisms. To investigate the molecular mechanisms of quinclorac resistance, we characterized the β‐CAS genes alongside plant transformation studies. The association of β‐CAS activity and ethylene production to quinclorac resistance was assayed in the F6 progeny of susceptible and resistant lines of E. phyllopogon. RESULTS A single nucleotide polymorphism in a β‐CAS1 intron deleted aberrantly spliced mRNAs and enhanced β‐CAS activity in the resistant line. The enhanced activity, however, was not associated with quinclorac resistance in F6 lines. The results were supported by lack of quinclorac resistance in Arabidopsis thaliana expressing E. phyllopogon β‐CAS1 and no difference in quinclorac sensitivity between β‐CAS knockout and wild‐type rice. Reduced ethylene production co‐segregated with quinclorac resistance in F6 lines which were previously characterized to be resistant to other herbicides by an enhanced metabolism. CONCLUSION β‐CAS does not participate in quinclorac sensitivity in E. phyllopogon. Our results suggest that a mechanism(s) leading to reduced ethylene production is behind the resistance. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quinclorac resistance in Echinochloa phyllopogon is associated with reduced ethylene synthesis rather than enhanced cyanide detoxification by β‐cyanoalanine synthase

Chayapakdee

Sunohara

Endo

et al. 2019

Pest Management Science

Self Cite

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“…Unfortunately, many studies do not continue to functionally validate these candidate genes and the actual cause for resistance remains undetermined. In successful validation studies, researchers have utilized Agrobacterium tumefaciens-mediated transformation of candidate genes in model plant systems, such as Arabidopsis thaliana [59][60][61][62], tobacco (Nicotiana benthamiana) [63], rice calli (Oryza sativa) [64,65], transgenic rice [66,67], or budding yeast (Saccharomyces cerevisiae) [68]. However, most weed science studies fall short of functionally validating identified genes due to lack of investment to date in stable plant transformation methods for weeds.…”

Section: Improved Gene Function Validation For Herbicide Resistance Mmentioning

confidence: 99%

“…More technologies should be developed to diagnose known resistance mechanisms, including nucleic acid probes, antibodies, and metabolite screens. More knowledge gaps exist for NTSR, with a few examples of known genetic variants for metabolic resistance characterized to date in weeds [61,64,93], but with many more cases of metabolic resistance to be discovered. With an improved understanding of metabolic resistance genes and pathways, transcriptional and/or protein markers can be screened as a diagnostic panel, in which the presence of defined subsets of markers indicates a sample is positive for metabolic resistance, similar to what is currently performed in cancer diagnostics [94,95].…”

Section: Where Is Weed Omics Going?mentioning

confidence: 99%

Omics Potential in Herbicide-Resistant Weed Management

Patterson

Saski

Küpper

et al. 2019

Plants

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The rapid development of omics technologies has drastically altered the way biologists conduct research. Basic plant biology and genomics have incorporated these technologies, while some challenges remain for use in applied biology. Weed science, on the whole, is still learning how to integrate omics technologies into the discipline; however, omics techniques are more frequently being implemented in new and creative ways to address basic questions in weed biology as well as the more practical questions of improving weed management. This has been especially true in the subdiscipline of herbicide resistance where important questions are the evolution and genetic basis of herbicide resistance. This review examines the advantages, challenges, potential solutions, and outlook for omics technologies in the discipline of weed science, with examples of how omics technologies will impact herbicide resistance studies and ultimately improve management of herbicide-resistant populations.

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Weed Science and Weed Management

2023

Weed Science and Weed Management in Rice and Cereal‐Based Cropping Systems

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Role of CYP81A cytochrome P450s in clomazone metabolism in Echinochloa phyllopogon

Cited by 54 publications

References 35 publications

Quinclorac resistance in Echinochloa phyllopogon is associated with reduced ethylene synthesis rather than enhanced cyanide detoxification by β‐cyanoalanine synthase

Quinclorac resistance in Echinochloa phyllopogon is associated with reduced ethylene synthesis rather than enhanced cyanide detoxification by β‐cyanoalanine synthase

Omics Potential in Herbicide-Resistant Weed Management

Weed Science and Weed Management

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