Role of cytokines (interleukin‐1β, 6, 8, tumour necrosis factor‐α, and soluble receptor of interleukin‐2) and C‐reactive protein in the diagnosis of neonatal sepsis

Reyes, Candelaria Santana; García-Muñoz, F; Reyes, Diana P; González, Guillermo; Domı́nguez, Carmen; Doménech, E

doi:10.1111/j.1651-2227.2003.tb00530.x

Cited by 85 publications

(43 citation statements)

References 34 publications

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“…This study reported increase in levels of CRP for both mothers and neonates in groups I as compared to group II with sensitivity, specificity, PPV and NPV of 88.6 and 84.7, 86.9 and 90.4%, respectively in neonates. These results are similar to that of other investigators (Reyes et al, 2003). In contrast to the results, Dollner et al (2002) found that CRP levels were low and undetectable in nearly all of the neonates, both in infectious and control groups.…”

Section: Discussionsupporting

confidence: 90%

Microbiological study of cases of early neonatal sepsis and evaluation of the role of C-reactive protein, interleukin-6 and interleukin-8 as diagnostic biomarkers of such cases

El-Mashad¹,

Amrousy²,

Sanosy³

et al. 2017

Afr. J. Microbiol. Res.

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Neonatal sepsis is responsible for significant morbidity and mortality. Its early diagnosis is very important but very difficult. This study aimed to evaluate the value of C reactive protein (CRP), interleukin 6 (IL-6) and interleukin 8 (IL-8) as early diagnostic biomarkers for early neonatal sepsis (ENOS). Forty neonates with prenatal risk for neonatal sepsis with their mother were taken in this study (group I). Ten healthy neonates and their healthy mothers were taken as the control group (group II) in Tanta University Hospital in the period of March to December, 2016. White blood count (WBC), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated in cord blood of neonates and in mother's sera. Also, cultures were done for neonates to confirm neonatal sepsis. The results of this study showed that the gestational age was shorter in the study group than the control group. Staphylococcus aureus represent 55% of the organisms isolated from cases of early neonatal sepsis. WBC, ESR, CRP, IL-6 and IL-8 were significantly higher in the study group than the control group. In the study group, 50% had positive cultures, while there were only 10% in thr control group with P value of 0.001. The sensitivity, specificity, positive predictive value (PPV) and negative predictive values (NPV) for CRP were 88.6, 84.7, 86.9 and 90.4%, for IL-6, they were 92.4, 97.6, 90.4 and 86.6%, for IL-8, they were 90.8, 88.9, 92.4 and 91.7%, respectively. The best sensitivity and NPV was for IL-6. Cord blood CRP alone has little utility in EONS diagnosis. IL-6 and IL-8 has great superiority than CRP when combined with other hematological markers. IL-6 was better diagnostic biomarker for ENOS than IL-8 and CRP.

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Section: Discussionsupporting

confidence: 90%

Microbiological study of cases of early neonatal sepsis and evaluation of the role of C-reactive protein, interleukin-6 and interleukin-8 as diagnostic biomarkers of such cases

El-Mashad¹,

Amrousy²,

Sanosy³

et al. 2017

Afr. J. Microbiol. Res.

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“…The ability to readily measure IL-8 in human samples, combined with its well-defined biological role, has provided the foundation for investigations centered on IL-8 as a biomarker in sepsis and septic shock. Multiple studies have demonstrated the potential utility of IL-8 in the early diagnosis of systemic bacterial infections, particularly in neonates (17)(18)(19)(20)(21). Of more direct relevance to the current study, previous studies have correlated increased serum IL-8 levels with increased illness severity and mortality in adults and children with septic shock (22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34).…”

Section: Discussionmentioning

confidence: 71%

Interleukin-8 as a Stratification Tool for Interventional Trials Involving Pediatric Septic Shock

Wong¹,

Cvijanovich²,

Wheeler³

et al. 2008

Am J Respir Crit Care Med

133

103

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Rationale: Interventional clinical trials involving children with septic shock would benefit from an efficient preenrollment stratification strategy. Objectives: To test the predictive value of interleukin (IL)-8 for 28-day mortality in pediatric septic shock. Methods: A training data set (n 5 40) identified a serum IL-8 of greater than 220 pg/ml as having a 75% sensitivity and specificity for predicting 28-day mortality. This cutoff was then subjected to a series of validation steps. Measurements and Main Results: Subjects were drawn from two large, independent pediatric septic shock databases. Prospective application of the IL-8 cutoff to validation data set 1 (n 5 139) demonstrated 78% sensitivity and 64% specificity for 28-day mortality. A serum IL-8 level of 220 pg/ml or less, however, had a negative predictive value for 28-day mortality of 95% in validation data set 1, which was subsequently applied to an independently generated data set of children with septic shock (validation set 2, n 5 193). A serum IL-8 level of 220 pg/ml or less had a negative predictive value for 28-day mortality of 94% when applied to validation set 2. Conclusions: A serum IL-8 level of 220 pg/ml or less, obtained within 24 hours of admission, predicts a high likelihood of survival in children with septic shock. We propose that IL-8 can be used to exclude such patients from interventional clinical trials and ultimately derive a study population with a more favorable risk to benefit ratio when subjected to a study agent.

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“…24 A utilização da IL-1β como citocina para o diagnóstico de sepse é pequena devido aos relatos conflitantes da concentração sérica desta citocina ora elevada, ora diminuída, em associação à sepse. 25,26 . Apesar dos níveis séricos de IL-1β serem freqüentemente indetectáveis quando medidos na sepse (e por essa razão a literatura relata pouca correlação com a gravidade da doença) 20 , em alguns pacientes são observados níveis elevados e, quando isto acontece, existe correlação com a gravidade do quadro séptico.…”

Section: Discussionunclassified

Mediadores pró-inflamatórios e antinflamatórios na sepse neonatal: associação entre homeostase e evolução clínica

Cianciarullo

Ceccon

Yamamoto

et al. 2008

J. Hum. Growth Dev.

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Resumo:Objetivo: avaliar a utilidade de citocinas pró-inflamatórias (TNF-α, IL-1β e IL-6) e de citocinas antinflamatórias (IL-10 e IL-1Ra) no diagnóstico da sepse neonatal, e verificar se a homeostase entre estes mediadores poderia ser determinante para a evolução clínica da doença. Método: coorte prospectiva compreendendo 31 recém-nascidos (RN) com diagnóstico de sepse neonatal, classificados em dois grupos: sepse e sepse grave, com evolução complicada (choque, falência múltipla de órgãos, óbito). Os níveis séricos de TNF-α; IL-1β; IL-6; IL-10 e IL-1Ra foram mensurados nos dias 0 (diagnóstico), 3 e 7 (evolutivos). Foram calculadas as médias, desviospadrão, medianas, e valores mínimos e máximos para cada um dos mediadores. Foram construídos gráficos dos perfis individuais dos pacientes, e o perfil médio dos dois grupos contendo os errospadrão. Para o tratamento estatístico dos dados oriundos da avaliação das concentrações de citocinas ao longo do tempo, foi utilizado o teste ANOVA com medidas repetidas. Para todas as análises realizadas foi adotado nível de significância de 5%. Resultados: no grupo de recém-nascidos com sepse e boa evolução, os níveis séricos de TNF-α; IL-1β e Il-10 se apresentaram próximos aos valores mínimos detectáveis pelo método, e nos RN com sepse grave, esses níveis foram estatisticamente superiores (p<0,01). As concentrações de IL-6 e IL-1Ra analisadas, de forma evolutiva (dias zero, 3 e 7 após o diagnóstico), revelaram níveis séricos sempre elevados e maiores na sepse grave em relação a sepse com boa evolução (p<0,01). A relação IL-6/IL-1Ra nos RN com sepse mostrou predomínio da ação pró-inflamatória no dia 0 (razão >1) e da resposta antiinflamatória nos dia 3 e 7 de evolução (razão <1), enquanto na sepse grave e evolução complicada, houve predomínio da ação pró-inflamatória no dia zero e no dia 3 (razão >1) e somente no dia 7, houve predomínio da ação antiinflamatória (razão <1). Conclusões: as concentrações de

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Role of cytokines (interleukin‐1β, 6, 8, tumour necrosis factor‐α, and soluble receptor of interleukin‐2) and C‐reactive protein in the diagnosis of neonatal sepsis

Cited by 85 publications

References 34 publications

Microbiological study of cases of early neonatal sepsis and evaluation of the role of C-reactive protein, interleukin-6 and interleukin-8 as diagnostic biomarkers of such cases

Microbiological study of cases of early neonatal sepsis and evaluation of the role of C-reactive protein, interleukin-6 and interleukin-8 as diagnostic biomarkers of such cases

Interleukin-8 as a Stratification Tool for Interventional Trials Involving Pediatric Septic Shock

Mediadores pró-inflamatórios e antinflamatórios na sepse neonatal: associação entre homeostase e evolução clínica

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