Role of cytoskeleton and surface lectins inGiardia duodenalis attachment to Caco2 cells

Magne, Denis; Favennec, Loïc; Chochillon, Christian; Gorenflot, A.; Meillet, D; Kapel, Nathalie; Raichvarg, D; Savel, J; Gobert, J.G.

doi:10.1007/bf00928679

Cited by 40 publications

(26 citation statements)

References 12 publications

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“…" Preliminary experiments showed that the preincubation of Caco-2 cells with suspensions of L. johnsonii La1 did not inhibit the complex adhesion of trophozoites to cell monolayers (data not shown). This interaction involves the ventral disk, several contractile elements, and hydrodynamic and mechanical forces, as well as lectin-binding factors (18,21,24,25). The possibility remained that if an antagonistic effect of the bacteria against the parasite existed, it could result from the release of bacterial metabolites or products more than a competitive exclusion or steric hindrance (3).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Giardia intestinalis by Extracellular Factors from Lactobacilli: an In Vitro Study

Pérez

Minnaard

Rouvet

et al. 2001

Appl Environ Microbiol

105

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The aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. The growth of Giardia intestinalis strain WB, as well as the attachment to the human intestinal epithelial cell line Caco-2, was evaluated by using proliferation and adhesion assays with radiolabeled parasites. In addition, scanning electron microscopy and flow cytometric analysis were performed. The effect of spent culture supernatants from lactobacilli was strain dependent. Lactobacillus johnsonii La1 significantly inhibited the proliferation of G. intestinalis trophozoites. Although the effect was strongly pH dependent, it was not simply due to lactic acid. According to flow cytometric analysis, trophozoites were arrested in G 1 phase but neither significant necrosis nor apoptosis could be detected. Bacterial cells or their spent culture supernatants were unable to modify trophozoite attachment to Caco-2 cells. However, trophozoites treated with spent culture supernatants had little, if any, proliferative capacity. These results suggest that La1 produces some substance(s) able to inhibit proliferation of Giardia trophozoites. Partial characterization of the factors involved in the antigiardiasic action showed that they have a low molecular mass and are inactivated by heating. On this basis, it seems worthwhile to explore how colonization of the proximal small bowel with these lactic acid bacteria could interfere with giardiasis in vivo.

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Section: Discussionmentioning

confidence: 99%

Inhibition of Giardia intestinalis by Extracellular Factors from Lactobacilli: an In Vitro Study

Pérez

Minnaard

Rouvet

et al. 2001

Appl Environ Microbiol

105

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“…Using their flagella and ventral disc, trophozoites released in the upper part of the small intestine move to the microvillus-covered surface of the duodenum and jejunum, where they attach themselves (88,116), and play a role in the onset of the pathology (22,34,124). The suction force created by this mode of attachment may damage the microvilli and interfere with the process of food absorption (88,116). Eventually, the rapid multiplication of the trophozoites by binary fission creates a physical barrier between the intestinal epithelial cells and the lumen of the intestine, interfering with the process of absorption of nutrients.…”

Section: Giardiasismentioning

confidence: 99%

Immune Response to Giardia duodenalis

Faubert¹

2000

Clinical Microbiology Reviews

196

132

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“…1A), with normal confluence levels and negligible differences in detachment forces compared to those with plain glass. While it is likely that specific binding is important for parasite-host recognition or interactions in vivo (14), specific binding cannot explain Giardia's robust attachment to treated glass surfaces.…”

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confidence: 99%

Giardia lamblia Attachment Force Is Insensitive to Surface Treatments

Hansen

Tulyathan²,

Dawson

et al. 2006

Eukaryot Cell

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Giardia lamblia cell populations show 90% detachment from glass under normal forces of 2.43 ؎ 0.33 nN applied by centrifugation. Detachment forces were not significantly different for cells attached to positively charged, hydrophobic, or inert surfaces than for cells attached to plain glass. The insensitivity of attachment force to surface treatment is consistent with a suction-based mechanism of attachment.For many parasitic organisms, the ability to bind tightly to host tissue is necessary for pathogenicity and survival. One such organism is Giardia lamblia (synonymous with G. intestinalis and G. duodenalis), a protistan parasite of the mammalian small intestine, which attaches securely but reversibly to the microvillus brush border of the host intestinal epithelium. Giardia is the most frequently identified cause of human intestinal infection in the United States and worldwide (5,15,23).The ventral disk, a structure unique to Giardia, is a 9-mdiameter concave spiral of cross-linked microtubules and associated proteins across the anterior underside of the cell. Attachment mechanisms proposed usually indicate the ventral disk as the principal source of attachment force (3). Proposed mechanisms of attachment to hard surfaces can be grouped into two categories: (i) binding and (ii) suction mechanisms. Binding models require close contact between a portion of the cell (such as the ventral disk or the cell periphery) and the substrate in order to generate attachment force via molecularlevel interactions. Possible binding-based mechanisms include nonspecific interactions (electrostatic and van der Waals), hydrophobic interactions, or ligand-mediated specific binding. In contrast, suction-based mechanisms are those requiring a pressure differential under the ventral disk for generation of attachment force.Previous theoretical and experimental studies of Giardia attachment (reviewed in reference 3) have revealed many aspects of cell behavior at surfaces; however, inconsistencies in methods and a lack of quantitative data on attachment forces have allowed only limited conclusions to be drawn about the mechanism of attachment. It is known that Giardia cells attach easily and reversibly in vitro to a variety of surfaces, including glass and polystyrene, allowing us to ask two important questions that provide insight into the mechanism of attachment. How strongly do Giardia trophozoites attach to a model surface in vitro? How does attachment change with surface treatments?We developed a centrifugation technique to measure the force of attachment of Giardia cell populations on plain glass and chemically modified substrates. The use of this simplified system allows separation of physical attachment from molecular interactions with host cells, enabling greater control of substrate surface properties, ease of modeling, and comparison to other eukaryotic cell-glass adhesion results. Additionally, centrifugation offers the advantage of being able to apply uniform normal forces to large populations of cells. The method is simila...

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Role of cytoskeleton and surface lectins inGiardia duodenalis attachment to Caco2 cells

Cited by 40 publications

References 12 publications

Inhibition of Giardia intestinalis by Extracellular Factors from Lactobacilli: an In Vitro Study

Inhibition of Giardia intestinalis by Extracellular Factors from Lactobacilli: an In Vitro Study

Immune Response to Giardia duodenalis

Giardia lamblia Attachment Force Is Insensitive to Surface Treatments

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