Role of decursin in TNF-α-stimulated migration, invasion, and matrix metalloproteinase-9 induction in vascular smooth muscle cells

Park, Sung-Suk; Kim, Wun-Jae; Moon, Sung‐Kwon

doi:10.1007/s10068-011-0126-2

Cited by 2 publications

(2 citation statements)

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“…Platelet-derived growth factor (PDGF) is a homodimer (AA and BB) and a heterodimer (AB) [ 5 ]. The abnormal motility of VSMC is stimulated by several growth factors such as insulin-like growth factor, transforming growth factor-β, and platelet-derived growth factor BB (PDGF-BB) [ 5 , 6 , 7 ]. Downstream from these pathways are reactive oxygen species (ROS), which are crucial for to regulating the abnormal motility of VSMC during arterial pathogenesis [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Artemisinin attenuates plantlet-derived growth factor BB-induced migration of vascular smooth muscle cells

et al. 2014

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BACKGROUND/OBJECTIVESArtemisinin (AT), an active compound in Arternisia annua, is well known as an anti-malaria drug. It is also known to have several effects including anti-oxidant, anti-inflammation, and anti-cancer activities. To date, the effect of AT on vascular disorders has not been studied. In this study, we investigated the effects of AT on the migration and proliferation of vascular smooth muscle cells (VSMC) stimulated by platelet-derived growth factor BB (PDGF-BB).MATERIALS/METHODSAortic smooth muscle cells were isolated from Sprague-Dawley rats. PDGF-BB stimulated VSMC migration was measured by the scratch wound healing assay and the Boyden chamber assay. Cell viability was determined by using an EZ-Cytox Cell Viability Assay Kit. The production of reactive oxygen species (ROS) in PDGF-BB stimulated VSMC was measured through H2DCF-DA staining. We also determined the expression levels of signal proteins relevant to ROS, including measures of extracellular signal-regulated kinase (ERK) 1/2 measured by western blot analysis and matrix metalloproteinase (MMP) 9 measured by reverse transcription-polymerase chain reaction (RT-PCR).RESULTSAT (10 µM and 30 µM) significantly reduced the proliferation and migration of PDGF-BB stimulated VSMC in a dose-dependent manner. The production of ROS, normally induced by PDGF-BB, is reduced by treatment with AT at both concentrations. PDGF-BB stimulated VSMC treated with AT (10 µM and 30 µM) have reduced phosphorylation of ERK1/2 and inhibited MMP9 expression compared to untreated PDGF-BB stimulated VSMC.CONCLUSIONSWe suggest, based on these results, that AT may exert an anti-atherosclerotic effect on PDGF-BB stimulated VSMCs by inhibiting their proliferation and migration through down-regulation of ERK1/2 and MMP9 phosphorylation.

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Section: Introductionmentioning

confidence: 99%

“…Matrix metalloproteinase (MMP) 9 has been reported as a key enzyme in vascular pathogenesis and in embryogenesis [ 7 ]. Also, under the oxidative stress MMP9 contributes to the abnormal cell migration of VSMC that leads to ECM degradation and remodeling in the vascular endothelium [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Artemisinin attenuates plantlet-derived growth factor BB-induced migration of vascular smooth muscle cells

et al. 2014

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Chemical Constituents of the Radices of Angelica tenuissima

2014

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Angelica tenuissima Nakai (=Ligusticum tenuissimum Kitagawa, Umbelliferae) is widely used to treat headache, diarrhea, epilepsy, and rheumatic arthralgias in oriental traditional medicine [1]. There have been phytochemical reports on various types of compounds from this plant, including phthalides, coumarins, terpenoids, and phenylpropanoids [2, 3]. Some of these compounds were found to have diverse biological activities such as antioxidative [3], anticancer [4], angiotensin converting enzyme (ACE) inhibitory [5], and antifungal [6] effects. In the present study, eight compounds, 3-butylidene-4-hydroxyphthalide (1) [7], senkyunolide B (2) [8], senkyunolide C (3) [8], wallichilide (4) [9], calycanthoside (5) [10], 2-methoxy-2-(4c-hydroxyphenyl)ethanol (6) [11], succinic acid (7) [12], and docosanoic acid (8) [13] were isolated from A. tenuissima by various chromatographic techniques. The structures of 1-8 were identified by interpretation of their 1D and 2D NMR spectroscopic data as well as by comparison with the published values. To the best of our knowledge, this is the first report on the isolation of compounds 1-8 from A. tenuissima.The dried radices of A. tenuissima (13 kg) were extracted with MeOH (3 u 18 L) overnight at room temperature. The extracts were concentrated in vacuo at 40qC to afford a MeOH-soluble residue (2.7 kg) which was dissolved in water and continuously partitioned with solvents and then concentrated to give extracts of hexanes (604 g), EtOAc (30 g), n-BuOH (116 g), and water (445 g) layer, respectively. The hexane extracts (604 g) were separated by vacuum liquid column chromatography ( 18 u 20 cm; silica gel 230-400 mesh) using gradient mixtures of EtOAc in hexanes (0.5o100%) as mobile phases, affording eight fractions (HF-01-HF-08). Compounds 8 (100 mg) and 7 (25 mg) were obtained from HF-04

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Role of decursin in TNF-α-stimulated migration, invasion, and matrix metalloproteinase-9 induction in vascular smooth muscle cells

Cited by 2 publications

References 24 publications

Artemisinin attenuates plantlet-derived growth factor BB-induced migration of vascular smooth muscle cells

Artemisinin attenuates plantlet-derived growth factor BB-induced migration of vascular smooth muscle cells

Chemical Constituents of the Radices of Angelica tenuissima

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