Role of Drosophila in Human Disease Research 2.0

Yamaguchi, Masamitsu; Yamamoto, Shinya

doi:10.3390/ijms23084203

Cited by 8 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Drosophila is easy to maintain and manipulate in the laboratory. Drosophila has a relatively short generation time, and production of a large number of offspring guarantees statistical significance of the obtained experimental data (Table 2) [104][105][106]. Mutants, overexpression and RNAi lines for many of the Drosophila replicative DNA polymerase genes are available from various stock centres.…”

Section: Possible Application Of Drosophila Models To Study Human Dis...mentioning

confidence: 93%

“…In Drosophila, cancer models have been established and used to study various aspects of cancer research [107]. Drosophila tumour suppressor lgl cooperates with scribble and discs sufficiently large to maintain apicobasal polarity of epithelial cells [106]. Their mutations induce disorganization of epithelial tissue architecture followed by formation of multi-layered metastatic tumours.…”

Section: Possible Application Of Drosophila Models To Study Human Dis...mentioning

confidence: 99%

See 1 more Smart Citation

Association of Mutations in Replicative DNA Polymerase Genes with Human Disease: Possible Application of Drosophila Models for Studies

Yamaguchi¹,

Cotterill

2023

IJMS

Self Cite

View full text Add to dashboard Cite

Replicative DNA polymerases, such as DNA polymerase α-primase, δ and ε, are multi-subunit complexes that are responsible for the bulk of nuclear DNA replication during the S phase. Over the last decade, extensive genome-wide association studies and expression profiling studies of the replicative DNA polymerase genes in human patients have revealed a link between the replicative DNA polymerase genes and various human diseases and disorders including cancer, intellectual disability, microcephalic primordial dwarfism and immunodeficiency. These studies suggest the importance of dissecting the mechanisms involved in the functioning of replicative DNA polymerases in understanding and treating a range of human diseases. Previous studies in Drosophila have established this organism as a useful model to understand a variety of human diseases. Here, we review the studies on Drosophila that explored the link between DNA polymerases and human disease. First, we summarize the recent studies linking replicative DNA polymerases to various human diseases and disorders. We then review studies on replicative DNA polymerases in Drosophila. Finally, we suggest the possible use of Drosophila models to study human diseases and disorders associated with replicative DNA polymerases.

show abstract

Section: Possible Application Of Drosophila Models To Study Human Dis...mentioning

confidence: 93%

Section: Possible Application Of Drosophila Models To Study Human Dis...mentioning

confidence: 99%

Association of Mutations in Replicative DNA Polymerase Genes with Human Disease: Possible Application of Drosophila Models for Studies

Yamaguchi¹,

Cotterill

2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has been suggested that the minimum number of animals in a study is 3, but the maximum should be 6 or 7 [64]. In the future, the development of in vitro models mimicking humane physiological systems or in vivo model with less ethical concerns (such as drosophila) could assist Original Research this study [65]. Moreover, our extensive knowledge could make direct intervention human subjects possible.…”

Section: Future Directionmentioning

confidence: 99%

Chemometric-empowered spectroscopic techniques in pharmaceutical fields: A bibliometric analysis and updated review

Zulkifli,

Fakri,

Odigie

et al. 2023

Narra X

View full text Add to dashboard Cite

Undeniable increase in pharmaceutical demand has encouraged researchers to develop analytical techniques to analyze drugs and monitor their effects. Chemometrics enabled simple spectroscopic approaches (such as infrared or UV spectroscopy) to analyze complex samples, including drug formulations, excreted fluids and tissues of living organisms. The aim of this study was to evaluate the research trend of this combinatorial technique utilized for pharmaceutical-related sample analysis using a bibliometric analysis. Bibliometric data of published literature from the Scopus database on March 14, 2023 were retrieved using the keyword combinations of "multivariate", "chemometrics", "pattern recognition", "drug", "pharmaceutical" and "spectroscopy". Network visualization analysis was performed using VOSviewer on the co-occurring keywords and authorships, presenting data such as top cited papers (n=10). The literature review was performed based on the research trend revealed by the clusters that emerged in the network visualization. The analysis revealed that the first paper was published in 1973 (n=1) and a total of 3544 records have been published as of March 14, 2023, comprising original research articles (n=3144, 88.71%) and review articles (n=232, 6.55%). The keyword “chemometrics” with Total Link Strength (TLS) of 826 emerged as the most abundant, followed by “metabolomics” (TLS=388), “Raman spectroscopy” (TLS=280), “metabonomics” (TLS=272), “nuclear magnetic resonance” or “NMR” (TLS=271), and “multivariate analysis” (TLS=254). Network visualization revealed that the research falls into two general categories: (1) drug toxicity and efficacy monitoring and (2) quality control of drug manufacturing. The top cited paper (n=3269) was a review article published in 1999 describing the utility of nuclear magnetic resonance combined with multivariate statistics for metabolite profiling of biological samples. The chemometric-empowered spectroscopy techniques were expected to provide objective measurement during clinical studies and monitoring of therapeutic effects.

show abstract

“…The advent of wearable devices or recent advances in a comprehensive monitoring platforms have made longitudinal studies using human and mammalian models much easier. However, invertebrate models such as the fruit fly ( Drosophila melanogaster ) continue to be useful as models for understanding human diseases and aging due to their short lifespan, the availability of powerful genetic manipulation tools, and the conservation of biological processes and signaling pathways between mammals [ 111 , 112 ]. Although fruit flies show cellular senescence phenotypes [ 113 , 114 , 115 ], flies are not the best model for studying cellular senescence in the context of aging, as most cells in adult flies are postmitotic.…”

Section: Dnb Analysis In Metabolismmentioning

confidence: 99%

New Possibilities for Evaluating the Development of Age-Related Pathologies Using the Dynamical Network Biomarkers Theory

Akagi,

Koizumi,

Kadowaki

et al. 2023

Cells

View full text Add to dashboard Cite

Aging is the slowest process in a living organism. During this process, mortality rate increases exponentially due to the accumulation of damage at the cellular level. Cellular senescence is a well-established hallmark of aging, as well as a promising target for preventing aging and age-related diseases. However, mapping the senescent cells in tissues is extremely challenging, as their low abundance, lack of specific markers, and variability arise from heterogeneity. Hence, methodologies for identifying or predicting the development of senescent cells are necessary for achieving healthy aging. A new wave of bioinformatic methodologies based on mathematics/physics theories have been proposed to be applied to aging biology, which is altering the way we approach our understand of aging. Here, we discuss the dynamical network biomarkers (DNB) theory, which allows for the prediction of state transition in complex systems such as living organisms, as well as usage of Raman spectroscopy that offers a non-invasive and label-free imaging, and provide a perspective on potential applications for the study of aging.

show abstract

Role of Drosophila in Human Disease Research 2.0

Abstract: The fruit fly Drosophila melanogaster is a highly tractable animal model to study various human diseases [...]

Cited by 8 publications

References 13 publications

Association of Mutations in Replicative DNA Polymerase Genes with Human Disease: Possible Application of Drosophila Models for Studies

Association of Mutations in Replicative DNA Polymerase Genes with Human Disease: Possible Application of Drosophila Models for Studies

Chemometric-empowered spectroscopic techniques in pharmaceutical fields: A bibliometric analysis and updated review

New Possibilities for Evaluating the Development of Age-Related Pathologies Using the Dynamical Network Biomarkers Theory

Contact Info

Product

Resources

About